Technical Reference #1532

1532.

Membrane-Type 1 Matrix Metalloproteinase Regulates Cell Migration During Zebrafish Gastulation:Evidence For An Enteraction With Non-Canonical Wnt Signaling Rebecca C Coyle; Andrew Latimer; Jason R. Jessen, Vanderbilt Univeristy, Experimental Cell Research, 314(1532), (2008)
Link To Paper

Abstract:
Key to invasiveness is the ability of tumor cells to modify the extracellular matrix becomemotile and engage in directed migration towards the vasculature. One significant proteinassociatedwith metastatic progression is membrane-type 1 matrix metall

Keywords:
Cancer; gastrulation; membrane-type 1 matrix; metalloproteinase; polarity; Van Gogh-like 2; Wnt; zebrafish

Materials & Methods:
Wild-type zebrafish (Danio rerio) and knym119 mutant lines were maintained under standard laboratory conditions. Embryos were collected from natural matings reared at 28.5 °C and staged according to age and morphology as described [25].

Microscopic Technique
Fluorescence Microscopy, Time Lapse

Cell Type(s)
Zebrafish embryos