Technical Reference #1529

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
collagen-coated glass-bottom culture dishes (MatTek)

1529.

Prolonged Rxfp1 And Rxfp2 Signaling Can Be Explained By Poor Internalization And A Lack Of {Beta}-Arrestin Recruitment Gabrielle Elizabeth Callander; Walter G Thomas; Ross A D Bathgate, University of Melbourne, American Journal of Physiology: Cell Physiology, 296(1529), (2009)
Link To Paper

Abstract:
Relaxin induces sustained physiological responses which brings into question thedeactivation processes typical of most G protein-coupled receptors (GPCR) for its receptorrelaxin family peptide receptor 1 (RXFP1). Here we examined relaxin-dependent

Keywords:
G protein-coupled receptor; relaxin; INSL3

Materials & Methods:
DNA constructs. pRc/CMV/NHA-AT1R was made previously (47). To allow immunoprecipitation of AT1R the influenza HA epitope (YPYDVPDYA) which is recognized by the monoclonal antibody 12CA5 is present at the N terminus of the receptor.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
COS-7, HEK-293, HEK-293T