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Technical Reference #1526

Glass Bottom Culture Dishes

This study used MatTek product(s):

P35GCol-1.5-14-C

Citation in paper containing MatTek reference:
collagen-coated glass-bottom culture dishes (MatTek)

1526.

Microtubule And Cell Contact Dependency Of Er-Bound Ptp1B Localization In Growth Cones Federico Fuentes; Carlos O. Arregui, Universidad de San Martin, Molecular Biology of the Cell, 20(1526), (2009)
Link To Paper

Abstract:
PTP1B is an ER-bound protein tyrosine phosphatase implied in the regulation of celladhesion. Here we investigated mechanisms involved in the positioning and dynamics ofPTP1B in axonal growth cones and evaluated the role of this enzyme in axons. In gr

Materials & Methods:
DMEM NeurobasalTM Medium F12 N2 B27 ITS L-glutamine gentamicin penicillinstreptomycin trypsin fetal bovine serum horse serum and laminin were from Invitrogen (Carlsbad CA).

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence, Time-lapse

Cell Type(s)
Neurons - Hippocampal, retinal cells