Technical Reference #1524

1524.

Unimpaired Lysosomal Acidification In Respiratory Epithelial Cells In Cystic Fibrosis Peter Haggie; A.s Verkman, University of California, The Journal of Biological Chemistry, 284(1524), (2009)
Link To Paper

Abstract:
The mechanisms remain uncertain by which mutations inCFTR cause lung disease in cystic fibrosis (CF). Teichgra¨ber etal. recently reported increased ceramide in CF lungs which wasproposed to result from defective lysosomal acidification in airway

Materials & Methods:
Cell Lines—MDCK type II cells (kidney proximal tubule epithelial cells that do not express CFTR (9)) HT29 cells (CFTRexpressing colorectal adenocarcinoma epithelium ATCC HTB-38) and Calu-3 cells

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
MDCK