Technical Reference #1523

1523.

Requirements For Numa In Maintenance And Establishment Of Mammalial Spindle Poles AD Silk; AJ Holland; DW Cleveland, University of California, The Journal of Cell Biology, 184(1523), (2009)

Abstract:
Microtubules of the mitotic spindle in mammaliansomatic cells are focused at spindle poles aprocess thought to include direct capture by astralmicrotubules of kinetochores and/or noncentrosomallynucleated microtubule bundles. By construction and

Materials & Methods:
A mouse 129S6/SvEvTac bacterial artifi cial chromosome genomic library (RPCI-22; Children ’ s Hospital Oakland Research Institute Oakland CA) was screened for the NuMA gene with a probe to the largest exon of NuMA.

Microscopic Technique
Fluorescence Microscopy, Live Cell

Cell Type(s)
MEF