Technical Reference #1522

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glassbottom dishes precoated with poly- L -lysine (MatTek)

1522.

Bcl-X L Increases Mitochondrial Fission, Fusion, And Biomass In Neurons SB Berman, YB Chen, B Qi, JM McCaffery, EB Rucker 3rd, S Goebbels, KA Nave, BA Arnold, EA Jonas, FJ Pineda, JM Hardwick, Johns Hopkins University, The Journal of Cell Biology, 184(1522), 716, (2009)
Link To Paper

Abstract:
Mitochondrial fi ssion and fusion are linked tosynaptic activity in healthy neurons and areimplicated in the regulation of apoptotic celldeath in many cell types. We developed fl uorescencemicroscopy and computational strategies to directlymeasu

Materials & Methods:
Floxed bcl-x mice ( Wagner et al., 2000 ) were bred with NEX-CRE knockin mice ( Goebbels et al., 2006 ) to generate conditional bcl-x knockout ( bcl-x fl ox / fl ox ;nex cre/+ ) and control embryos ( bcl-x fl ox / + ;nex cre/+ ).

Microscopic Technique
Electron Microscopy

Cell Type(s)
Neurons, control mouse neurons