Technical Reference #1515

1515.

Calcium/Calmodulin Signaling Elicits Release Of Cytochrome C During 2;3;7;8-Tetrachlorodibenzo-P-Dioxin-Induced Apoptosis In The Human Lymphoblastic T-Cell Line; L-Mat Daisuke Kobayashi; Sohel Ahmed; Masato; Ishida; Shuya Kasai; Hideaki Kikuchi, Iwate University, Toxicology, 258(1515), (2009)

Abstract:
We have reported previously that 2378-tetrachlorodibenzo-p-dioxin (TCDD) induces apoptosis in the human lymphoblastic T-cell line L-MAT although these cells do not express the aromatic hydrocarbon receptor (AhR). The AhR-dependent pathway for the indu

Keywords:
Calcium; Calmodulin; Dioxin; Apoptosis; Lymphoblastic T-cell line

Materials & Methods:
L-MAT cells were cultured as described previously (Hossain et al. 1998) with some modifications.Briefly the cells were grown in RPMI 1640 medium (MP Biomedical Inc. Irvine CA) containing 25mMHEPES pH 7.4 5% fetal bovine serum 100 IU/ml penici

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
L-MAT