Technical Reference #1513

1513.

Generation Of Hvem-Restricted Hsv-1 Mutant Viruses: Resistance Of Hvem-Expressing Cells And Identification Of Mutations That Rescue Nectin-1 Recognition Hiroaki Uchida; Waris A. Shah; Ali Ozuer; Arthur R. Frampton; Jr.; William F. Goins; Paola Grandi; Justus B. Cohen; and Joseph C. Glorioso, University of Pittsburgh, The Journal of Virology, 83(1513), (2009)
Link To Paper

Abstract:
Both initial infection and cell-to-cell spread by herpes simplex virus type 1 (HSV-1) require interaction of the viral glycoprotein D (gD) with an entry receptor on the cell surface. The two major HSV entry receptors HVEM and nectin-1 mediate infection

Materials & Methods:
For evaluation of gD receptor expression test cells were plated onto 35-mm glass-bottom 197 culture dishes (MatTek Ashland MA) fixed with 4% paraformaldehyde at RT for 30 min

Microscopic Technique
Fluorescence Microscopy, Immunofluorescence

Cell Type(s)
HT-29