Technical Reference #1507

1507.

Sanguinarine Cytotoxicity On Mouse Melanoma K1735-M2 Cells—Nuclear Vs. Mitochondrial Effects Teresa L. Serafim; Júlio A.C. Matos; Vilma A. Sardão; Gonçalo C. Pereira; Ana F. Branco; Sandro L. Pereira; Donna Parke; Edward L. Perkins; António J.M. Moreno; Jon Holy; Paulo J. Oliveira, University of Coimbra, Biochemical Pharmacology, 76(1507), (2008)

Abstract:
Sanguinarine (SANG) is an alkaloid recognized to have anti-proliferative activity againstvarious human tumour cell lines. No data is available on the susceptibility of advancedmalignant melanoma to SANG although this disease has a very poor prognosis

Keywords:
Sanguinarine K1735–M2 rat melanoma cells Liver mitochondria Mitochondrial physiology Cell proliferation Mitochondrial permeability transition Bioenergetics Apoptosis

Materials & Methods:
Cells were seeded in glass-bottom dishes (P35G-1.5-14-C MatTek Corporation Ashland MA) at a concentration of 1– 2 104 cells/ml.

Microscopic Technique
Fluorescence Microscopy, Epifluorescence Microscopy

Cell Type(s)
K1735-M2