Technical Reference #1502

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass bottom microwell dishes (MatTek Corporation)

1502.

Novel Interactions Of Cln3 Protein Link Batten Disease To Dysregulation Of Fodrin–Na+; K+ Atpase Complex Kristiina Uusi-Rauva; Kaisu Luiro; Kimmo Tanhuanpää; Outi Kopra; Pablo Martín-Vasallo; Aija Kyttälä; Anu Jalanko, Institute for Molecular Medicine, Experimental Cell Research, 314(1502), (2008)

Abstract:
Juvenile neuronal ceroid lipofuscinosis (JNCL Batten disease) is the most common progressiveneurodegenerative disorder of childhood. CLN3 the transmembrane protein underlying JNCL isproposed to participate in multiple cellular events including memb

Keywords:
Batten disease JNCL CLN3 Fodrin Na+; K+ ATPase TIRF microscopy Primary neurons Ouabain-induced endocytosis

Materials & Methods:
Cells grown on glass bottom microwell dishes (MatTek Corporation) were either treated with 0.85 mM ouabain (Fluka) at 37 °C for 15 min (ouabaininduction) or fixed immediately (basal condition)

Microscopic Technique
Fluorescence Microscopy, Total Internal Reflection Fluorescence (TIRF)

Cell Type(s)
fibroblasts