Technical Reference #1500

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom plates (MatTek)

1500.

Whamm Is An Arp2/3 Complex Activator That Binds Microtubules And Functions In Er To Golgi Transport Kenneth G. Campellone; Neil J. Webb; Elizabeth A. Znameroski; Matthew D. Welch, University of California Berkeley, Cell, 134(1500), (2008)
Link To Paper

Abstract:
The Arp2/3 complex is an actin nucleator thatplays a critical role in many cellular processes. Itsactivities are regulated by nucleation-promoting factors(NPFs) that function primarily during plasmamembrane dynamics. Here we identify a mammalian

Materials & Methods:
For live-cell studies transfected cells grown on glass-bottom plates (MatTek) were transferred into DMEM 5% FBS 25 mM HEPES (pH 7.4) and Oxyrase to inhibit phototoxicity.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
COS-7, 356HFF, HeLa