Technical Reference #1498

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
uncoated #1.5 glass-bottomed culture dishes (MatTek)

1498.

Changes In Bicoid Mrna Anchoring Highlight Conserved Mechanisms During The Oocyte-To-Embryo Transition Timothy T. Weil; Richard Parton; Ilan Davis; Elizabeth R. Gavis, Princeton University, Current Biology, 18(1498), (2008)
Link To Paper

Abstract:
Intracellular mRNA localization directs protein synthesis toparticular subcellular domains to establish embryonic polarityin a variety of organisms. In Drosophila bicoid (bcd)mRNA is prelocalized at the oocyte anterior.

Materials & Methods:
Individual stage 14 oocytes from well-fed females were dissected in Schneider’s insect culture medium (GIBCO-BRL) transferred to uncoated #1.5 glass-bottomed culture dishes (MatTek)

Microscopic Technique
Confocal Microscopy

Cell Type(s)
stage 14 oocytes