Technical Reference #1494

1494.

Differential Effect Of Sulfonylureas On Production Of Reactive Oxygen Species And Apoptosis In Cultured Pancreatic Β-Cell Line; Min6 Fumi Sawada; Toyoshi Inoguchi; Hirotaka Tsubouchi; Shuji Sasaki; Masakazu Fujii; Yasutaka Maeda; Hidetaka Morinaga; Masatoshi Nomura; Kunihisa Kobayashi; Ryoichi Takayanagi, Kyushu University, Metabolism Clinical and Experimental, 57(1494), (2008)
Link To Paper

Abstract:
Here we examined the effects of various sulfonylureas and a short-acting insulin secretagoguenateglinide on oxidative stress and apoptosis using the β-cell line MIN6.

Materials & Methods:
For experiments the cells were placed into glass-bottom culture dishes (MatTek Ashland MA) and cultured in DMEM containing 15% FBS and 25 mmol/L glucose.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
MIN6