Technical Reference #1493

1493.

Quantitative Profiling Of Nucleotides And Related Phosphate-Containing Metabolites In Cultured Mammalian Cells By Liquid Chromatography Tandem Electrospray Mass Spectrometry Rebecca L. Cordell; Stephen J. Hill; Catharine A. Ortori; David A. Barrett, University of Nottingham, Journal of Chromatography B, 871(1493), (2008)

Abstract:
A method has been developed for the quantitative profiling of over twenty nucleotides and related phosphorylatedspecies using ion-pair reversed-phase liquid chromatography hyphenated to negative iontandem electrospray mass spectrometry. The influence

Keywords:
LC–MS/MS Nucleotides Metabolite profiling Chinese hamster ovary cells

Materials & Methods:
The cells were grown for 48 h on Mattek dishes with a coverslip base using standard culture conditions as described previously.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
CHO