Technical Reference #1490

1490.

The Chemokine Ccl2 Activates P38 Mitogen-Activated Protein Kinase Pathway In Cultured Rat Hippocampal Cells Jungsook Cho; Donna L. Gruol, The Scripps Research Institute, Journal of Neuroimmunology, 199(1490), (2008)

Abstract:
Emerging evidence indicates that chemokines can regulate both the physiology and biochemistry of CNS neurons and glia. In the current studyWestern blot analysis showed that in rat hippocampal neuronal/glial cultures the signal transduction pathway acti

Keywords:
Chemokines; CREB; MAP kinase pathways; Hippocampal neurons; CCR2 1. Introduction The chemokine CCL2; a 14 kDa protein previously known as monocyte

Materials & Methods:
The cell suspension was plated at a density of 1 hippocampus per glass bottom culture dish (for immunohistochemistry (MatTek Corporation Ashland MA USA)

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
Neurons - Hippocampal