Technical Reference #1478

1478.

Elevated Glucose Changes The Expression Of Ionotropic Glutamate Receptor Subunits And Impairs Calcium Homeostasis In Retinal Neural Cells Ana R. Santiago; Susana C. Rosa; Paulo F. Santos; Armando J. Cristóvão; Alistair J. Barber; and António F. Ambrósio, University of Coimbra, Investigative Ophthalmology and Visual Science, 47(1478), (2006)
Link To Paper

Abstract:
Altered glutamatergic neurotransmission and calciumhomeostasis may contribute to retinal neural cell dysfunctionand apoptosis in diabetic retinopathy (DR). The purpose of thisstudy was to determine the effect of high glucose on theprotein content

Materials & Methods:
For Ca2 imaging studies cells were plated on 35-mm glass-bottomed culture dishes (MatTek; Ashland MA). The cells were maintained at 37°C in a humidified incubator with 5% CO2/air.

Microscopic Technique
Fluorescence Microscopy, Calcium Imaging

Cell Type(s)
R28