Technical Reference #1476

1476.

Upregulation Of Ephb2 And Ephrin-B2 At The Optic Nerve Head Of Dba/2J Glaucomatous Mice Coincides With Axon Loss Juan Du; Tony Tran; Christine Fu; and David W. Sretavan, University of California San Francisco (UCSF), Investigative Ophthalmology and Visual Science, 48(1476), (2007)
Link To Paper

Abstract:
To identify genes with upregulated expression at theoptic nerve head (ONH) that coincides with retinal ganglioncell (RGC) axon loss in glaucomatous DBA/2J mice. To furtherdemonstrate that the proteins encoded by these genes bind toRGC axons and in

Materials & Methods:
After an interval of 6 to 12 days the retinas were dissected and cut into pieces approximately 1 mm2 and placed vitreous side down on 35-mm glass-bottomed tissue culture dishes (MatTek Corp. Ashland MA) precoated with poly-L-lysine

Microscopic Technique
Confocal Microscopy, Laser Scanning, Calcium Imagin

Cell Type(s)
DBA/2J