Technical Reference #1476

1476.

Upregulation Of Ephb2 And Ephrin-B2 At The Optic Nerve Head Of Dba/2J Glaucomatous Mice Coincides With Axon Loss Juan Du, Tony Tran, Christine Fu, and David W. Sretavan, University of California, San Francisco (UCSF), Investigative Ophthalmology and Visual Science, 48(1476), 5569, (2007)
Link To Paper

Abstract:
To identify genes with upregulated expression at theoptic nerve head (ONH) that coincides with retinal ganglioncell (RGC) axon loss in glaucomatous DBA/2J mice. To furtherdemonstrate that the proteins encoded by these genes bind toRGC axons and in

Materials & Methods:
After an interval of 6 to 12 days, the retinas were dissected and cut into pieces approximately 1 mm2 and placed vitreous side down on 35-mm glass-bottomed tissue culture dishes (MatTek Corp., Ashland, MA) precoated with poly-L-lysine

Microscopic Technique
Confocal Microscopy, Laser Scanning, Calcium Imagin

Cell Type(s)
DBA/2J