Technical Reference #1473

1473.

Laminin And Biomimetic Extracellular Elasticity Enhance Functional Differentiation In Mammary Epithelia Jordi Alcaraz; Ren Xu; Hidetoshi Mori; Celeste M Nelson; Rana Mroue; Virginia A Spencer; Doug Brownfield; Derek C Radisky; Carlos Bustamante and Mina J Bissell, University of California Berkeley, EMBO Journal, 27(1473), (2008)
Link To Paper

Abstract:
In the mammary gland epithelial cells are embedded in a‘soft’ environment and become functionally differentiated inculture when exposed to a laminin-rich extracellular matrixgel. Here we define the processes by which mammaryepithelial cells inte

Materials & Methods:
For AFM experiments 100 ml of ECM solutions were added to glass-bottomed culture dishes (MatTek) and incubated for 30 min at 37 1C to allow gelation unless otherwise indicated.

Microscopic Technique
Fluorescence Microscopy, Inverted Fluorescence Microscopy

Cell Type(s)
SCp2