Technical Reference #1472

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-D-lysine coated glass-bottom dishes (MatTek)

1472.

The Kv2.1 K+ Channel Targets To The Axon Initial Segment Of Hippocampal And Cortical Neurons In Culture And In Situ Sarmiere PD; Weigle CM; Tamkun MM, Colorado State University, BMC Neuroscience, 9(1472), (2008)
Link To Paper

Abstract:
Transfected and endogenous Kv2.1 is now demonstrated to preferentially accumulatewithin the axon initial segment (AIS) over other neurite processes; 87% of 14 DIV hippocampalneurons show endogenous channel concentrated at the AIS relative to the soma

Materials & Methods:
Briefly neurons from cryo-preserved E18 rat hippocampal dissociations were plated at a density of ~15000 – 30000 cells/cm2 on poly-D-lysine coated glass-bottom dishes (MatTek)

Microscopic Technique
Confocal Microscopy

Cell Type(s)
E18