Technical Reference #1470

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
glass-bottom dishes (poly-d-lysine coated; No. 1.5; MatTek Corp.; Ashland; MA)

1470.

Membrane Potential Controls Adipogenic And Osteogenic Differentiation Of Mesenchymal Stem Cells Sarah Sundelacruz; Michael Levin; David L. Kaplan, Tufts University, PLoS ONE, 3(1470), (2008)
Link To Paper

Abstract:
We examined the membrane potential (Vmem) changes exhibited by human mesenchymal stem cells (hMSCs) undergoing adipogenic (AD) and osteogenic (OS) differentiation and uncovered a characteristic hyperpolarization of differentiated cells versus undifferent

Materials & Methods:
Cells grown in glass-bottom dishes (poly-d-lysine coated No. 1.5 MatTek Corp. Ashland MA) were incubated in DiSBAC for 30 minutes at 37°C then imaged while submerged in dye at room temperature.

Microscopic Technique
Confocal Microscopy, Laser Scanning

Cell Type(s)
hMSCs