Technical Reference #1464

1464.

The Dynamics Of Mammalian P Body Transport; Assembly And Disassembly In Vivo Adva Aizer*; Yehuda Brody*; Ler Lian Wee†; Nahum Sonenberg†; Robert H. Singer‡; and Yaron Shav-Tal*, Bar-Ilan University, Molecular Biology of the Cell, 19(1464), (2008)
Link To Paper

Abstract:
Exported mRNAs are targeted for translation or can undergo degradation by several decay mechanisms. The 5’􀃆3’ degradation machinery localizes to cytoplasmic P bodies (PBs). We followed the dynamic properties of PBs in vivo and investigated the mechanism

Keywords:
mRNA degradation; cytpoplasmic P bodies (PBs)

Materials & Methods:
For time-lapse imaging cells were plated on glass-bottomed tissue culture plates (MatTek Ashland MA) in medium containing 10% FCS at 37°C.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
U2OS