Technical Reference #1457

1457.

Physiological Role For Phosphatidic Acid In The Translocation Of The Novel Protein Kinase C Apl Ii In Aplysia Neurons Carole A. Farah; Ikue Nagakura; Daniel Weatherill; Xiaotang Fan; and Wayne S. Sossin, Montreal Neurological Institute, Molecular and Cellular Biology, 28(1457), (2008)
Link To Paper

Abstract:
In Aplysia californica the serotonin-mediated translocation of protein kinase C (PKC) Apl II to neuronal membranes is important for synaptic plasticity. The orthologue of PKC Apl II PKC has been reported to require phosphatidic acid (PA) in conjunction

Keywords:
PKC; synaptic facilitation; Aplysia californica; Ca2+; translocation

Materials & Methods:
For transfection cells were plated on MatTek glassbottom culture dishes (MatTek Corporation Ashland MA) with a glass surface of 14 mm and a coverslip thickness of 0.16 to 0.19 mm at a density of 0.11 106 cells/35-mm dish.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Sf9