Technical Reference #1456

1456.

Prokineticin 2 Influences Subfornical Organ Neurons Through Regulation Of Map Kinase And The Modulation Of Sodium Channels Mark Fry; G. Trevor Cottrell and Alastair V. Ferguson, Queen’s University, American Journal of Physiology: Regulatory; Integrative and Comparative Physiology, 295(1456), (2008)
Link To Paper

Abstract:
Prokineticin 2 (PK2) is a neuropeptide which acts as an signaling molecule regulating circadian rhythms in mammals. We have previously reported PK2 actions on subfornical organ (SFO) neurons identifying this circumventricular organ as a target at which PK

Keywords:
circumventricular organs; neuropeptides; potassium current; sodium current; MAPK; intracellular Ca2+

Materials & Methods:
The supernatant was again removed cells were resuspended and plated in glass bottomed culture dishes for imaging (MatTek Corporation Ashland MA) or 35mm plastic culture dishes for electrophysiology (Corning Corning NY).

Microscopic Technique
Fluorescence Microscopy, Calcium Imaging

Cell Type(s)
SFO neurons