Technical Reference #1455

1455.

The Alpha1D-Adrenergic Receptor Induces Vascular Smooth Muscle Apoptosis Via A P53-Dependent Mechanism Mary L Garcia-Cazarin; Jennifer L Smith; Daret K St Clair; Michael T Piascik, University of Kentucky Lexington, Molecular Pharmacology, 74(1455), (2008)
Link To Paper

Abstract:
Activation of the endogenous alpha1-adrenergic receptor (AR) associated human aortic smooth muscle cells resulted in a dose and time-dependent increase in the levels of mitochondrial reactive oxygen species (ROS).

Keywords:
Adrenergic; Gq/11 family; Immunocytochemistry; Apoptosis; Reactive intermediates

Materials & Methods:
Human aortic smooth muscle cells were plated in 35mm glass bottom culture dishes (MatTek Corporation Ashland MA). To assess the role of the alpha-AR on apoptosis the cells were treated with phenylephrine for 12 hrs.

Microscopic Technique
Confocal Microscopy, Fluorescence

Cell Type(s)
aortic smooth muscle