Technical Reference #1454

1454.

A Novel Actin-Related Protein Is Associated With Daughter Cell Formation In Toxoplasma Jennifer L. Gordon; Wandy L. Beatty; and L. David Sibley, Washington University, Eukaryotic Cell, 7(1454), (2008)
Link To Paper

Abstract:
Cell division in Toxoplasma gondii occurs by an unusual budding mechanism termed endodyogeny during which twin daughters are formed within the body of the mother cell. Cytokinesis begins with the coordinated assembly of the inner membrane complex (IMC)

Keywords:
Toxoplasma; endodyogeny; Arps; ALPS; actin

Materials & Methods:
HFF cell monolayers grown on glass microwell 35 mm dishes (MatTek Cultureware Ashland MA) were inoculated with parasites expressing either GFP(2)-ALP1 alone or both GFP(2)-ALP1 and IMC1-Tomato(2) and grown for ~ 18 hours.

Microscopic Technique
Confocal Microscopy, Laser Scanning, Temperature controlled

Cell Type(s)
HFF