Technical Reference #1444

1444.

Plasma Membranes Are Poised For Activation Of Raft Phase Coalescence At Physiological Temperature Daniel Lingwood; Jonas Ries; Petra Schwille; and Kai Simons, Technical University of Dresden, PNAS, 105(1444), (2008)
Link To Paper

Abstract:
Cell membranes are not randomly organized but rather are populated by fluctuating nanoassemblies of increased translational order termed lipid rafts. This lateral heterogeneity can be biophysically extended because cooling formaldehyde-isolated plasma me

Keywords:
cholera toxin; clustering; ganglioside; lateral sorting

Materials & Methods:
PMS Formation: A431 cells were grown to 60–80% confluence in DMEM in 35-mm glass-bottom live-cell imaging dishes (P35G-1.5-10-C; MatTek).

Microscopic Technique
Live Cell Microscopy

Cell Type(s)
A431