Technical Reference #1440

1440.

Regulation Of Nuclear Import/Export Of Chrebp; Interaction Of An -Helix Of Chrebp With The 14-3-3 Proteins And Regulation By Phosphorylation Haruhiko Sakiyama; R. Max Wynn; Wan-Ru Lee; Masashi Fukasawa; Hiroyuki Mizuguchi; Kevin H. Gardner; Joyce J. Repa; and Kosaku Uyeda, University of Texas Southwestern Medical Center, The Journal of Biological Chemistry, 283(1440), (2008)
Link To Paper

Abstract:
Carbohydrate response element binding protein (ChREBP) is a glucose-responsive transcription factor that plays a critical role in the glucose-mediated induction of gene products involved in hepatic glycolysis and lipogenesis. Glucose affects the activity

Keywords:
ChREBP; glucose; phosphorylation; 14-3-3

Materials & Methods:
hepatocytes were plated onto 35 mm glassbottomed dishes (MatTek) coated with type I collagen at a density of 5.0 X 105 cells per dish

Microscopic Technique
Confocal Microscopy, Laser Scanning

Cell Type(s)
Hepatocytes