Technical Reference #1437

1437.

Paracrine Effects Of Hypoxic Fibroblast-Derived Factors On The Mpt-Ros Threshold And Viability Of Adult Rat Cardiac Myocytes Kailasam Shivakumar; Steven J. Sollott; Mohan Sangeetha; Subrayan Sapna; Bruce Ziman; Su Wang; and Edward G. Lakatta, Sree Chitra Tirunal Institute for Medical Sciences and Technology, American Journal of Physiology: Heart and Circulatory Physiology, 294(1437), (2008)
Link To Paper

Abstract:
Cardiac fibroblasts contribute to multiple aspects of myocardial function and pathophysiology. The pathogenetic relevance of cytokine production by these cells under hypoxia however remains unexplored. Using an in vitro cell culture model this study ev

Keywords:
mitochondrial permeability transition-reactive oxygen species threshold; cardiac myocyte viability; cardiac fibroblasts; hypoxia; fibroblast-conditioned medium; fibroblast proliferation; apoptosis; necrosis; TNF-a

Materials & Methods:
Cells were suspended in HEPESbuffered solution containing (in mmol/l) 137 NaCl 4.9 KCl 1.2 MgSO4 1.2 NaH2PO4 15 glucose 20 HEPES and 1.0 CaCl2 pH 7.2 and seeded on MatTek plates at 4 104 cells/cm2.

Microscopic Technique
Confocal Microscopy

Cell Type(s)
Myocytes