Technical Reference #127

127.

Quantitative Imaging Of Green Fluorescent Protein In Cultured Cells: Comparison Of Microscopic Techniques; Use In Fusion Proteins And Detection Limits Niswender; K.D.; Blackman; S.M.; Rohde; L.; Magnuson; M.A.; and Piston; D.W., Vanderbilt University, Journal of Microscopy, 180(127), (1995)
Link To Paper

Abstract:
To determine the application limits of green fluorescent protein (GFP) as a reporter gene or protein tag we expressed GFP by itself and with fusion protein partners and used three different imaging methods to identify GFP fluorescence. In conventional e

Microscopic Technique
Fluorescence Microscopy, Epifluorescence Microscopy, Quantitative fluorescence microscopy, Scanning laser microscopy

Cell Type(s)
Hela