Technical Reference #1056

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
35-mm glassbottom Microwell culture dishes (MatTek; Ashland; MA)

1056.

Bax Interacts With The Voltage-Dependent Anion Channel And Mediates Ethanol-Induced Apoptosis In Rat Hepatocytes Masayuki Adachi; Hajime Higuchi; Soichiro Miura; Toshifumi Azuma; Sayaka Inokuchi; Hidetsugu Saito; Shinzo Kato; and Hiromasa Ishii, National Defense Medical College Japan, AJP - Gastrointestinal and Liver Physiology, 287(1056), (2004)
Link To Paper

Abstract:
Acute ethanol exposure induces oxidative stress and apoptosis in primary rat hepatocytes. Previous data indicate that the mitochondrial permeability transition (MPT) is essential for ethanol-induced apoptosis.

Keywords:
alcoholic liver disease; mitochondria; oxidative stress; cytochrome c

Materials & Methods:
Briefly cultured rat hepatocytes on 35-mm glassbottom Microwell culture dishes (MatTek Ashland MA) were incubated with DMEM (pH 7.4) containing 1 M DCFH-DA for 30 min at 37°C in the dark.

Microscopic Technique
Fluorescence Microscopy

Cell Type(s)
cultured rat hepatocytes