Technical Reference #1053

Glass Bottom Culture Dishes

This study used MatTek product(s):

Citation in paper containing MatTek reference:
poly-L-lysine-coated cover glass bottom dishes (catalog no. P35GC-1.5-14-C; MatTek; Ashland; MA)

1053.

The Human Papillomavirus E7 Protein Deregulates Mitosis Via An Assocation With The Nuclear Mitotic Apparatus Protein-1 (Numa) Christine L. Nguyen and Karl Münger, Brigham & Women's Hospital, The Journal of Virology, 83(1053), (2009)
Link To Paper

Abstract:
We have previously observed that high-risk human papillomavirus (HPV) type 16 (HPV16) E7 expression leads to the delocalization of dynein from mitotic spindles (Nguyen C.L. McLaughlin-Drubin M.E. and Munger K. 2008. Canc. Res. 68: 8715-22.)

Keywords:
Human papillomavirus; E7 oncoprotein; NuMA; Mitosis; Genomic Instability

Materials & Methods:
~2x106 3 cells were plated on poly-L-lysine coated coverglass bottom dishes (MatTek Ashland 4 MA CatNo. P35GC-1.5-14-C) 26 hours prior to imaging.

Microscopic Technique
Inverted Microscopy

Cell Type(s)
C33a, CaSki, 3T3-CE7, 3T3-poz