Technical Reference #1002

1002.

Fluorescence Correlation Spectroscopy And Fluorescence Cross-Correlation Spectroscopy Reveal The Cytoplasmic Origination Of Loaded Nuclear Risc In Vivo In Human Cells Thomas Ohrt, Jörg Mütze, Wolfgang Staroske, Lasse Weinmann, Julia Höck, Karin Crell, Gunter Meister, and Petra Schwille, Dresden University of Technology, Max Planck Institute of Biochemistry, Germany, Nucleic Acids Research, 36(1002), 6440, (2008)
Link To Paper

Abstract:
Studies of RNA interference (RNAi) provide evidence that in addition to the well-characterized cytoplasmic mechanisms, nuclear mechanisms also exist. The mechanism by which the nuclear RNA-induced silencing complex (RISC) is formed in mammalian cells, as

Materials & Methods:
For microinjection, 7–9104 ER293/10G cells were transferred onto MatTek chambers coated with Fibronectin (25 mg/ml in PBS including CaCl2 and MgCl2, Roche) 24 h before microinjection.

Microscopic Technique
Laser Scanning Microscopy, Microinjection

Cell Type(s)
EGFP-Ago2, ER293