Technical Reference #1002

1002.

Fluorescence Correlation Spectroscopy And Fluorescence Cross-Correlation Spectroscopy Reveal The Cytoplasmic Origination Of Loaded Nuclear Risc In Vivo In Human Cells Thomas Ohrt; Jörg Mütze; Wolfgang Staroske; Lasse Weinmann; Julia Höck; Karin Crell; Gunter Meister; and Petra Schwille, Dresden University of Technology, Nucleic Acids Research, 36(1002), (2008)
Link To Paper

Abstract:
Studies of RNA interference (RNAi) provide evidence that in addition to the well-characterized cytoplasmic mechanisms nuclear mechanisms also exist. The mechanism by which the nuclear RNA-induced silencing complex (RISC) is formed in mammalian cells as

Materials & Methods:
For microinjection 7–9104 ER293/10G cells were transferred onto MatTek chambers coated with Fibronectin (25 mg/ml in PBS including CaCl2 and MgCl2 Roche) 24 h before microinjection.

Microscopic Technique
Laser Scanning Microscopy, Microinjection

Cell Type(s)
EGFP-Ago2, ER293