| MICROSCOPIC TECHNIQUE | TITLE | HYPERLINKTR NO | ABSTRACT | KEYWORDS | MATERIALS & METHODS | JOURNAL | VOL | YEAR | MONTH | ISSUE | MATTEK DISH | SPECIES | TISSUE | ATCC DESC | MORPHOLOGY | CELL LINE | MAIN AUTHOR | CONTRIBUTING AUTHORS | COMPANY | |||||||
| Acoustic Microscopy | ||||||||||||||||||||||||||
| Optical And Ultrasonic Monitoring Of Laser-Generated Intracellular Contrast Agents: Initial Cell Culture Studies | 384 | Acoustically monitored laser-induced optical breakdown (LIOB) can be used its an important diagnostic and therapeutic tool in living cells. With properly controlled laser parameters, optical breakdown is a minimally invasive means to target a single, tran | laser-induced
optical breakdown (LIOB), photodisruption |
CHO-K1 (Chinese
hamster ovary) cells were used as the in vitro target tissue for laser photodisruption. Cells were cultured in a self-adherent monolayer onto a No. 0 coverslip in a glass-bottom culture dish (MatTek Corporation, Ashland, MA). Cells were gr |
Ultrasonics Symposium 2004 IEEE | 2 | 2004 | August | CHINESE HAMSTER | OVARY | ECACC 85051005 Morphology: Epithelial Hamster Chinese ovary Depositor: Dr D Newell, PHLS CAMR, Porton Down, Salisbury, UK No restrictions. Patent: None Specified By Depositor Properties: Applications: Nutritional and gene expression studies |
epithelial-like | CHO | Zohdy, Marwa J. | Marwa J. Zohdy, Christine Tse, Jing Yong Ye, and Matthew O’Donnell | University of Michigan | ||||||||||
| Analytical microscopy | ||||||||||||||||||||||||||
| Conformational Changes In Hiv-1 Gp41 In The Course Of Hiv-1 Envelope Glycoprotein-Mediated Fusion And Inactivation | 364 | HIV-1 envelope glycoprotein-mediated fusion is driven by the concerted coalescence of the HIV-1 gp41 N- and C-helical regions, which results in the formation of 6-helix bundles. These two regions are considered prime targets for peptides and antibodies th | glycoprotein-mediated fusion, HIV-1-mediated cell-cell fusion, HIV-1 gp41, immunogenic | HeLa cells were
plated in 12 well plates, 105 cells per well, 24 h prior to infection. The vaccinia stock (20 íL of approximately 109 plaque forming units/mL) was mixed with 30 íL of 0.25 mg/mL trypsin solution in Dulbecco’s phosphate-buffered saline |
P35GCOL-0-10-C | HUMAN | cervix | IZSBS BS TCL20 Morphology: epithelial-like Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology |
epithelial-like | HeLa | Dimitrov, Antony S. | Antony S. Dimitrov, John M. Louis, Carole A. Bewley, G. Marius Clore, and Robert Blumenthal | National Institutes of Health | |||||||||||||
| Mechanism Of Prion Propagation: Amyloid Growth Occurs By Monomer Addition Amyloid Growth Occurs By Monomer Addition | 357 | Abundant nonfibrillar oligomeric intermediates are a common feature of amyloid formation, and these oligomers, rather than the final fibers, have been suggested to be the toxic species in some amyloid diseases. Whether such oligomers are critical intermed | prion
inheritance, polymerization, NM, a fragment of the Sup35 protein
containing the glutamine/asparagine-rich N-terminal (N) and highly charged middle (M) domains, amyloid formation |
For continuous
thioflavin T assay measurements,
concentrated stocks of NM stored in either 6 M GuHCl (fluorescently labeled NM and NM used for Figures 2C, 2F, and 6) or 4 M GuHCl and 4 M urea (NM used for all other experiments) were diluted at least 2 |
PLoS Biology | 2 | 2004 | September | 9 | P35G-1.5-14-C | Coleoptiles victoriae from oat | Collins, Sean R. | Sean R. Collins, Adam Douglass, Ronald D. Vale, Jonathan S. Weissman | HHMI | ||||||||||||
| Novel Dynamic Rheological Behavior Of Individual Focal Adhesions Measured Within Single Cells Using Electromagnetic Pulling Cytometry | 426 | The rheology of
cells and sub-cellular structures, such as focal adhesions, are important for
cell form and function. Here we describe electromagnetic pulling cytometry (EPC), a technique to analyze cell rheology by applying dynamic tensional forces to |
Cell mechanics; Cytoskeleton; Focal adhesion; Creep; Magnetic | Bovine capillary
endothelial cells were cultured in 10% CO2 at 37 C on gelatin-coated tissue culture dishes in Dulbeccos modified Eagles medium (DMEM, Gibco-BRL) supplemented with 10% calf serum (CS; Hyclone), 10 mM Hepes (JRH-Biosciences), 2 mM L |
Acta Biomaterialia | 1 | 2005 | May | 3 | BOVINE | ALVEOLAR TYPE II | Overby, Darryl R. | Darryl R. Overby, Benjamin D. Matthews, Eben Alsberg, Donald E. Ingber | Children's Hospital Boston | ||||||||||||
| Surface Probe Measurements Of The Elasticity Of Sectioned Tissue, Thin Gels And Polyelectrolyte Multilayer Films:Correlations Between Substrate Stiffness And Cell Adhesion | 483 | Surface probe
measurements of the elasticity of thin film matrices as well as biological
samples prove generally important to understanding cell attachment across such systems. To illustrate this, sectioned arteries were probed by atomic force microscop |
Surface structure, morphology, roughness, and topography; Adhesion; Atomic force microscopy | A7r5 SMCs
(aorta-derived cell line) were maintained in polystyrene flasks between passages 2 and 15, and cultured in media containing DMEM, supplemented with 10% of fetal bovine serum and antibiotics. Cells were passed every 3 days, when reaching 80 |
Surface Science | 570 | 2004 | October | 1-2 | MUSCLE | A7R5 | Engler, Adam J. | Adam J. Engler,
Ludovic Richert, Joyce Y. Wong, Catherine Picart, Dennis E. Discher |
University of Pennsylvania | ||||||||||||
| Axioscope Fluorescence Microscopy | ||||||||||||||||||||||||||
| Activation of erbB-1 Signaling in Tanycytes of the Median Eminence Stimulates Transforming Growth Factor 1 Release via Prostaglandin E2 Production and Induces Cell Plasticity | 496 | The activation
of transforming growth factor
(TGF)– erbB-1 and neuregulin– erbB-4 signaling pathways in
hypothalamic astrocytes has been shown to play a key role in the process by which the neuroendocrine brain controls luteinizing hormone-releasing ho |
neuroendocrine; TGF ; TGF; ependymoglial cells; LHRH; cell plasticity; hypothalamus | Tanycytes
were seeded in cloning cylinders positioned on 37 mm culture chambers (MatTek Corp., Ashland, MA) containing poly-L-ornithine- and laminin-coated glass coverslips, as described above. After removal of the cylinder, the cells were maintained |
Journal of Neuroscience | 23 | 2003 | November | 33 | Tanycytes | Prevot, Vincent | Vincent Prevot, Anda Cornea, Alison Mungenast, Gregory Smiley, and Sergio R. Ojeda | Oregon Health and Sciences University | |||||||||||||
| Calcium Imaging | ||||||||||||||||||||||||||
| Astragaloside
IV inhibits spontaneous synaptic transmission and synchronized Ca2+ oscillations on hippocampal neurons |
897 | Aim: To
investigate the changes in the spontaneous neuronal excitability
induced by astragaloside IV (AGS-IV) in the cultured hippocampal network. Methods: Hippocampal neurons in culture for 9–11 d were used for this study. The spontaneous synaptic activi |
astragaloside
IV; synchronized spontaneous Ca 2+ osci l l at ion; spont aneous synapt i c transmission; voltage-gated Na+ currents; voltage-gated K+ currents |
In brief,
hippocampal tissues from 18 d fetal rats...were then dissociated by trituration with a glass Pasteur pipette and plated in 35 mm culture dishes with a glass bottom (MatTek, Ashland, MA, USA) for culture and subsequent microscopy. |
Acta Pharmacologica Sinica | 29 | 2008 | January | 1 | 35-mm glass bottom dishes | Hippocampal
neurons from embryonic Sprague-Dawley rats (E18) |
Shao-qing ZHU | Shao-qing ZHU, Lei QI, Yan-fang RUI, Ru-xin LI, Xiang-ping HE, Zuo-ping XIE | |||||||||||||
| Hydroxylation
Increases the Neurotoxic Potential of BDE-47 to Affect Exocytosis and Calcium Homeostasis in PC12 Cells |
881 | BACKGROUND:
Oxidative metabolism, resulting in the formation of hydroxylated
polybrominated diphenyl ether (PBDE) metabolites, may enhance the neurotoxic potential of brominated flame retardants. OBJECTIVE: Our objective was to investigate the effects of |
Bioactivation,
brominated flame retardants, calcium, catecholamine, exocytosis,
intracellular calcium stores, neurotoxicity, neurotransmitter release, persistent organic pollutants, polybrominated diphenyl ether. |
For Ca2+
imaging experiments, we subcultured undifferentiated PC12 cells in poly-L-lysine–coated glass-bottom dishes (MatTek, Ashland, MA, |
Environmental Health Perspectives | 116 | 2008 | May | 5 | poly-L-lysine–coated glass bottom dishes |
PC12 | Milou M.L. Dingemans | Milou M.L.
Dingemans, Aart de Groot, Regina G.D.M. van Kleef, Åke Bergman, Martin van den Berg, Henk P.M. Vijverberg, and Remco H.S. Westerink |
|||||||||||||
| Neonatal
Exposure to Brominated Flame Retardant BDE-47 Reduces Long-Term Potentiation and Postsynaptic Protein Levels in Mouse Hippocampus |
882 | BACKGROUND:
Increasing environmental levels of brominated flame retardants raise concern
about possible adverse effects, particularly through early developmental exposure. OBJECTIVE: The objective of this research was to investigate neurodevelopmental mec |
αCaMKII,
brain growth spurt, developmental neurotoxicity, field-EPSP recording, hippocampal synaptic plasticity, postsynaptic density. |
PC12 cells were
subcultured in poly-L-lysine–coated glass-bottom cell culture dishes (MatTek, Ashland MA, USA) |
Environmental Health Perspectives | 115 | 2007 | June | 6 | poly-L-lysine–coated
glass bottom dishes |
PC12 | Milou M.L. Dingemans | Milou M.L.
Dingemans, Geert M.J. Ramakers, Fabrizio Gardoni, Regina G.D.M. van Kleef,
Åke Bergman, Monica Di Luca, Martin van den Berg, Remco H.S. Westerink, and Henk P.M. Vijverberg |
|||||||||||||
| Pregnancy-Specific
Changes in Uterine Artery Endothelial Cell Signaling in Vivo Are Both Programmed and Retained in Primary Culture |
887 | Ovine uterine
artery (UA) endothelial cells (UAEC) maintained in culture to passage 4 retain pregnancy-specific changes in vasodilator production, which in turn is associated with differences in Ca2 and ERK 1/2 signaling. The question remains whether thi |
Ovine uterine artery (UA) endothelial cells (UAEC), Pregnancy-specific changes | Glass-bottom
microwell dishes for Ca2 imaging studies were purchased from MatTek Corporation (Ashland, MA), |
Journal of Endocrinology | 144 | 2003 | 8 | glass bottom dishes | UAEC | Shannon M. Gifford | Shannon M. Gifford, Jackie M. Cale, Stephen Tsoi, Ronald R. Magness, and Ian M. Bird | ||||||||||||||
| Confocal Fluorescence | ||||||||||||||||||||||||||
| Molecular Perspective of Antigen-mediated Mast Cell Signaling | 907 | Agonist
activation regulates reciprocal interactions of spinophilin and arrestin with the 2A- and 2B-adrenergic receptor (AR) subtypes via their 3i loop. Because arrestin association with G protein-coupled receptor is preceded by redistribution of arres |
HEK 293 or MEFs
were plated the night before the assay on MatTek dishes (35 mm) coated with 2.5 g/cm2 poly-D-lysine |
Molecular Pharmacology | 67 | 2005 | May | 5 | 35-mm glass bottom dishes | HEK 293 or MEFs | Ashley E. Brady | Ashley E. Brady,
Qin Wang, Patrick B. Allen, Mark Rizzo, Paul Greengard, and Lee E. Limbird |
||||||||||||||
| Confocal Laser | ||||||||||||||||||||||||||
| Close Homolog of L1 is an Enhancer of Integrin-mediated Cell Migration | 879 | Cells actively
produce contractile forces for a variety of processes including cytokinesis
and motility. Contractility is known to rely on myosin II motors which convert chemical energy from ATP hydrolysis into forces on actin filaments. However, the basi |
Actin, Active cytoskeletal networks, Contractility, Myosin II, Xenopus, alpha-actinin | Deposited onto
an inert oil layer (Fluorinert FC-40, 3M, St. Paul, MN; cat. No. F9755, Sigma, St. Louis, MO) on the bottom of a MatTek dish (model No. P35G-1.5-14-C, MatTek, Ashland, MA) equipped with a lid to minimize evaporation (Fig. 1 A). |
Biophysical Journal | 94 | 2008 | April | 8 | P35G-1.5-14-C | M-phase extracts (Xenopus) | Poul M. Bendix | Poul M. Bendix,
Gijsje H. Koenderink, Damien Cuvelier, Zvonimir Dogic, Bernard N.
Koeleman, William M. Brieher, Christine M. Field, L. Mahadevan, and David A. Weitz |
|||||||||||||
| Confocal Laser Scanning | ||||||||||||||||||||||||||
| Phosphorylation-dependent
Localization of Microtubule-associated Protein MAP2c to the Actin Cytoskeleton |
750 | IB kinase 2
(IKK2 or IKK) is a component of the IKK complex that coordinates the
cellular response to a diverse set of extracellular stimuli, including cytokines, microbial infection, and stress. In response to an external stimulus, the complex is activa |
Aurora A mitosis NF-B spindle polarity | HeLa cells expressing GFP-a-tubulin where reverse-transfected with siRNA IKK2 no. 2. Briefly, 80 nmol of the siRNA was diluted in 500 ml of OptiMem (Invitrogen) and placed in a 35-mm glass bottom dish (MatTek). | PNAS | 104 | 2007 | October | 43 | 35-mm glass bottom dishes | Human | Cervix | Epithelial | HeLa | Irelan, Jeffrey T. | Jeffrey T.
Irelan*, Thomas J. Murphy*, Paul D. DeJesus*, Hsiangling Teo, DingYue Xu,
Maria A. Gomez-Ferreria‡, Yingyao Zhou*, Loren J. Miraglia*, Daniel R. Rines*, Inder M. Verma§, David J. Sharp‡, Vinay Tergaonkar§, and Sumit K. Chanda |
Genomics Institute of the Novartis Research Foundation, †Institute for Molecular and Cell Biology, Albert Einstein College of Medicine, §The Salk Institute for Biological Studies | |||||||||
| Confocal Microscopy | ||||||||||||||||||||||||||
| 2-Adrenergic
Agonist Enrichment of Spinophilin at the Cell Surface Involves Subunits of Gi Proteins and Is Preferentially Induced by the 2A-Subtype |
787 | The HIV-1 Rev
protein is an essential regulator of the HIV-1 mRNA expression that promotes
the export of unspliced and partially spliced mRNA. The export receptor for the leucine-rich nuclear export signal (NES) of Rev has recently been recognized as CRM1 |
HLtat cells were
seeded onto coated 50-mm glass-bottom microwell dishes (MatTek), cultured in phenol red-free DMEM [supplemented with 10% (volvol) FCS] |
50-mm glass-bottom microwell dishes | Hltat | Daelemans, Dirk | Dirk Daelemans*,
Elena Afonina‡, Jakob Nilsson§, Gudrun Werner, Jørgen Kjems§, Erik De
Clercq*, George N. Pavlakis‡, and Anne-Mieke Vandamme |
Human Retrovirus
Section, National Cancer Institute, University of Aarhus, Novartis Research Institute |
||||||||||||||||||
| A Quantitative
Analysis of Contractility in Active Cytoskeletal Protein Networks |
759 | The human EGF
receptor (HER) 2 receptor tyrosine kinase is a survival factor for human
cardiomyocytes, and its inhibition may explain the increased incidence of cardiomyopathy associated with the anti-HER2 monoclonal antibody trastuzumab (Genentech, South |
monoclonal antibodies | Au565, MCF7,
MCF7/HER2, and HMCs cells were seeded on glass bottom dishes (MatTek, Ashland, MA) the day before the experiment. |
PNAS | 104 | 2007 | June | 25 | P35GC-1.0-14-C, P35G-1.0-14-C | Human | Breast cancer, Cardiomyocytes | Au565, MCF7, MCF7/HER2, and HMCs | Spector, Neil L. | Neil L.
Spector*, Yosef Yarden, Bradley Smith‡, Ljuba Lyass§, Patricia Trusk§, Karen
Pry§, Jason E. Hill§, Wenle Xia*, Rony Seger, and Sarah S. Bacus |
Duke University
Medical Center, Weizmann Institute, Cell Signaling Technologies, §Targeted Molecular Diagnostics |
||||||||||
| A role for IB kinase 2 in bipolar spindle assembly | 747 | Visual and
auditory hallucinations accompany certain neuropsychiatric disorders, such as
schizophrenia, and they also can be induced by the use or abuse of certain drugs. The heptahelical serotonin 2A receptors (5-HT2ARs) are molecular targets for drugind |
5-HT2A
receptor G protein-coupled
receptor internalization MAP kinase schizophrenia |
Neurons were
grown on poly-L-lysine-coated glass coverslip culture plates (Mat- Tek) at 37°C and in 5% CO2. |
PNAS | 105 | 2008 | January | 3 | Glass coverslip culture plates | Mouse | cortical neuronal | MEF | Schmid, Cullen L. | Cullen L. Schmid, Kirsten M. Raehal, and Laura M. Bohn | Ohio State University College of Medicine | ||||||||||
| A synthetic HIV-1 Rev inhibitor interfering with the CRM1-mediated nuclear export | 771 | Vaccination
against amyloid -peptide (A) has been shown to be successful in reducing A
burden and neurotoxicity in mouse models of Alzheimer’s disease (AD). However, although A immunization did not show T cell infiltrates in the brain of these mice, an A |
amyloid -peptide encephalitis vaccination | Microglia were cultured on MatTek Petri dishes for 24 h in the presence and absence of IFN-g. | PNAS | 103 | 2006 | March | 13 | Mouse | Microglial | Monsonego | Alon Monsonego*,
Jaime Imitola‡, Sanja Petrovic‡, Victor Zota‡, Anna Nemirovsky*, Rona Baron*,
Yair Fisher*, Trevor Owens§, and Howard L. Weiner |
Ben-Gurion University, Brigham and Women’s Hospital, Harvard Medical School, University of Southern Denmark | ||||||||||||
| Activation of
AMP-activated protein kinase by human EGF receptor 2/EGF receptor tyrosine
kinase inhibitor protects cardiac cells |
746 | Vascular
dysfunction has been reported in human cases of anthrax, in mammalian models
of Bacillus anthracis, and in animals injected with anthrax toxin proteins. To examine anthrax lethal toxin effects on intact blood vessels, we developed a zebrafish mod |
endothelial vascular permeability VEGF | For confocal
imaging of the heart: 15 minutes after bead injection, embryos were fixed in 2% PFA for 5–10 min; then embedded in 2% low melt agarose (Bio-Rad) on 35-mm glass bottom microwell dishes (MatTek Corp.). |
PNAS | 105 | 2008 | February | 7 | 35-mm glass
bottom microwell dishes |
Zebrafish | Heart | Endothelial | Harvard Medical School | ||||||||||||
| Agonist-directed signaling of the serotonin 2A receptor depends on -arrestin-2 interactions in vivo | 845 | Altered
calcium homeostasis is implicated in the pathogenesis of Alzheimer’s disease
(AD). Much effort has been put into understanding the association between protein mutations causative of this devastating neurodegenerative disease and perturbed calcium |
Alzheimer’s disease, APPswe, Ca, Spontaneous calcium oscillation, Hippocampal neuron | Mechanically
dispersed cells were plated on poly-d-lysine-coated 3.5cm glass bottom Petri dishes (Mattek Corporation) at a density of 450,000 cells/dish and cultured for 12–14 days at 37 ◦C and 5% CO2. |
Journal of Neuroscience | 436 | 2008 | May | 2 | P35G-0-14-C | Ppswe | Ewa Kloskowska | Ewa Kloskowska,,
Katarzyna Malkiewicza, Bengt Winblada, Eirikur Benedikza, Joseph D. Bruton |
|||||||||||||
| A-induced
meningoencephalitis is IFN-dependent and is associated with T cell-dependent
clearance of A in a mouse model of Alzheimer’s disease |
877 | Assessment of fat-specific protein 27 in the adipocyte lineage suggests a dual role for FSP27 in adipocyte metabolism and cell death. Am J Physiol Endocrinol Metab 294: E654–E667, 2008. First published January 15, 2008; doi:10.1152/ajpendo.00104.2007.—Fat |
insulin; tumor
necrosis factor-; adipogenesis; cell death-inducing DFF45-like effector; apoptosis; 3T3-L1 |
Confocal imaging
studies using MitoTracker Red CMX Ros (Invitrogen) were conducted on live cells by plating 3T3-L1 preadipocytes or COS cells onto laminin-coated MatTek glass bottom dishes (MatTek, Ashland, MA) |
American Journal of Physiology: Endocrinology and Metabolism | 294 | 2008 | April | 4 | glass bottom dishes | 3T3-L1 preadipocytes or COS cells | Ji Young Kim | Ji Young Kim, Kun Liu, Shengli Zhou, Kristin Tillison, Yu Wu, and Cynthia M. Smas | |||||||||||||
| Anthrax lethal toxin induces cell death-independent permeability in zebrafish vasculature | 816 | Caveolin-1 is a
principal component of caveolae membranes that may function as a
transformation suppressor. For example, the human caveolin-1 gene is
localized to a suspected tumor suppressor locus (D7S522; 7q31.1) that is deleted in human cancers, includ |
MTLn3
cells were plated on collagen I-coated MATTEK tissue culture dishes at a density of 2,500 cells/cm2. |
Journal of Biological Chemistry | 275 | 2000 | 27 | Mouse | Caveolin | MTLn3 | Zhang, Wei | Wei Zhang, Babak
Razania, Yoram Altschuler, Boumediene Bouzahzah, Keith E. Mostov, Richard G. Pestell, and Michael P. Lisanti |
Albert Einstein College of Medicine, University of California, San Francisco | |||||||||||||
| APPswe mutation
increases the frequency of spontaneous Ca2+-oscillations in rat hippocampal neurons |
711 | Background: The
interaction between macrophages and Cryptococcus neoformans (Cn) is
critical for containing dissemination of this pathogenic yeast. However, Cn can either lyse macrophages or escape from within them through a process known as phagosomal ex |
vacuole formation, Cryptococcus neoformans, murine macrophages | Cells were then
plated on polylysine coverslip-bottom MatTek plates (MatTek Cultureware, Ashland MA) at a density of 5 × 104 per plate in feeding media, |
BioMedCentral Immunology | 8 | 2007 | August | 16 | P35GC-0-14-C | Mice | macrophage-like | J774.16 | Alvarez, Mauricio | Mauricio Alvarez, Arturo Casadevall | Albert Einstein College of Medicine | ||||||||||
| Assessment of
fat-specific protein 27 in the adipocyte lineage suggests a dual role for FSP27 in adipocyte metabolism and cell death |
853 | Lipoteichoic
acid (LTA) is a central inducer of inflammatory responses caused by Grampositive bacteria, such as Staphylococcus aureus, via activation of TLR2. Localization of TLR2 in relation to its coreceptors may be important for function. This study ex |
TLR, LTA, endocytosis | MDCK cells
stably expressing Eea-1GFP were transiently transfected with the TLR2 overnight in Microwell dishes for microscopy (MatTek Corp.) |
Journal of Leukocyte Biology | 84 | 2008 | July | 35-mm glass bottom dishes | HEK293, MDCK | Nadra J. Nilsen | Nadra J.
Nilsen,* Susanne Deininger,† Unni Nonstad,* Frode Skjeldal,‡ Harald
Husebye,* Dmitrii Rodionov,‡ Sonja von Aulock,† Thomas Hartung,†,§ Egil Lien,¶ Oddmund Bakke,‡ and Terje Espevik |
||||||||||||||
| Caveolin-1
Inhibits Epidermal Growth Factor-stimulated Lamellipod Extension and Cell
Migration in Metastatic Mammary Adenocarcinoma Cells (MTLn3) |
827 | The molecular
mechanisms by which the AP-1 transcription factor c-Jun exerts its biological
functions are not clearly understood. In addition to its well established role in transcriptional regulation of gene expression, several reports have suggested tha |
For photoswitch
and fluorescence recovery after photobleaching (FRAP) studies, cells were transfected with PS-CFP2- DDX21, EGFP-DDX21 (15), or EGFP-DDX21 mut and grown on glass-bottom dishes (MatTek). |
Journal of Biological Chemistry | 283 | 2008 | March | 11 | P50G-1.5-14-F | Mouse | Embryo | HeLa, MEF | Holmstro, Tim H. | Tim H.
Holmstro¨m‡§, Antoine Mialon‡, Marko Kallio‡§, Yvonne Nymalm, Leni
Mannermaa‡, Tina Holm, Henrik Johansson, Elizabeth Black**, David Gillespie**‡‡, Tiina A. Salminen, U¨ lo Langel, Benigno C. Valdez§§, and Jukka Westermarck |
University of
Turku and Åbo Akademi University, University of Tampere and the Tampere
University Hospital, State Technical Research Center of Finland, University of Stockholm, University of Glasgow, University of Texas,MDAnderson Cancer Center |
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| Cell-to-cell spread and massive vacuole formation after Cryptococcus neoformans infection of murine macrophages | It is increasingly recognized that the compartmental organization of signaling processes has a profound influence on cellular behavior. However, our inability to influence these compartmental events in a spatially restricted and acute manner limits our un | Transfected HeLa cells were grown on MatTeK dishes. | MatTeK dishes | Human | HeLa | Fili, N. | N. Fili, V. Calleja, R. Woscholski, P. J. Parker,, and B. Larijani | Cancer Research UK, Imperial College London | ||||||||||||||||||
| Cellular
trafficking of lipoteichoic acid and Toll-like receptor 2 in relation to signaling; role of CD14 and CD36 |
795 | Purpose: Clinical confocal scanning laser microscopes are used in vivo to examine the cornea. However, such instruments are unable to yield the precision and resolution provided by research level in vitro instruments. This study was carried out to evaluat | cornea: basic science • cornea: epithelium • mitochondria | Following the labeling with fluorescent dyes, the corneal buttons were rinsed twice in HBSS and mounted in 1% agarose, previously dissolved in HBSS, on glass–bottom plates (MatTek Corp., Ashland, MA). | Investigative Opthamology & Visual Science | 2006 | P06G-1.5-20-F | Bovine | Cornea | Epithelial | Bantseev, V. | V. Bantseev and J.G. Sivak | University of Waterloo | |||||||||||||
| c-Jun Supports Ribosomal RNA Processing and Nucleolar Localization of RNA Helicase DDX21 | 724 | In
principle, cell surface receptors that are overexpressed in tumor tissue could serve as targets for anticancer drugs attached to receptor ligands. The purpose of this paper is to identify the necessary elements for a successful receptor-targeted drug. |
Cells were grown
in coated 50-mm cover glass bottom dishes (MatTek, Ashland, MA) in medium without phenol red. |
PNAS | 95 | 1998 | September | 20 | 50-mm cover glass bottom dishes | Human | gastrin I | NIH/3T3 | Czerwinski, Grzegorz | GRZEGORZ CZERWINSKI, NADYA I. TARASOVA, AND CHRISTOPHER J. MICHEJDA* | National Cancer Institute, Frederick Cancer Research and Development Center | |||||||||||
| Compartmental signal modulation: Endosomal phosphatidylinositol 3-phosphate controls endosome morphology and selective cargo sorting | 830 | Acoustic
overstimulation and ototoxic drugs can cause permanent hearing loss as a
result of the damage and death of cochlear hair cells. Relatively little is known about the signaling pathways triggered by such trauma, although a significant role has been |
cochlea; ototoxicity; calcium signaling; purinergic receptors; cell– cell communication; ERK | The cochlear
turns were placed onto Cell-Tak cell and tissue adhesive (BD Biosciences, San Jose, CA)-coated MatTek (Ashland, MA) dishes. |
Journal of Neuroscience | 19 | 2008 | May | P50G-0-14-F | Cochlea | Manuela Lahne | Manuela Lahne and Jonathan E. Gale | ||||||||||||||
| Confocal Laser Scanning Microscopy Imaging of the Bovine Corneal Epithelium in vitro | 743 | Va14Ja18
natural T (NKT) cells play an immunoregulatory role, which is controlled by a
self glycolipid(s) presented by CD1d. Although the synthetic antigen -D-galactosylceramide (-D-Gal- Cer) stimulates all Va14Ja18 NKT cells, -anomeric D-glycosylceram |
Cells (5
106) were plated in glass-bottom microwells (MatTek) and incubated overnight at 37°C to allow the cells to adhere to the wells. |
PNAS | 100 | 2003 | February | 4 | glass bottom dishes | Mouse | Va14Ja18 NKT | Stanic, Aleksandar | Aleksandar K.
Stanic*, A. Dharshan De Silva*, Jang-June Park*, Venkataraman Sriram‡§,
Shinichi Ichikawa, Yoshio Hirabyashi, Kyoko Hayakawa, Luc Van Kaer*, Randy R. Brutkiewicz‡§, and Sebastian Joyce |
Vanderbilt University School of Medicine, Yeshiva University, Indiana University School of Medicine, Walther Cancer Institute, Brain Science Institute, Fox Chase Cancer Center | ||||||||||||
| Cytotoxic agents directed to peptide hormone receptors: Defining the requirements for a successful drug | 874 | Calcium influx
through long-lasting (“L-type”) Ca2 channels (CaV) drives excitation-contraction in the normal heart. Dysregulation of this process contributes to Ca2 overload, and interventions that reduce expression of the pore-forming 1 subunit may a |
Functional Ca2+ Channels, Mutant B2, Cardiac Cells | After
trypsinization, dissociated cells were plated in glass culture dishes (MatTek, Ashland, MA) for electrophysiology (60–80 103 cells/ml). |
Journal of Pharmacology and Experimental Therapeutics | 325 | 2008 | April | 1 | glass bottom dishes | HL-1 cells | Sabine Telemaque | Sabine
Telemaque, Swapnil Sonkusare, Terrie Grain, Sung W. Rhee, Joseph R.
Stimers, Nancy J. Rusch, and James D. Marsh |
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| Damage-Induced Activation of ERK1/2 in Cochlear Supporting Cells Is a Hair Cell Death-Promoting Signal That Depends on Extracellular ATP and Calcium | 903 | The spatial
distributions of zinc, a representative transition metal, and active biomass
in bacterial biofilms were determined using two-photon laser scanning microscopy (2P-LSM). Application of 2P-LSM permits analysis of thicker biofilms than are amenabl |
Thin biofilms
were grown in microwell dishes with 0.17-mm glass coverslips at the bottom
(MatTek, Ashland, MA). |
Applied and Environmental Microbiology | 71 | 2005 | July | 7 | glass bottom dishes, 1.7mm thickness | PHL628 | Zhiqiang Hu | Zhiqiang Hu,
Gabriela Hidalgo, Paul L. Houston, Anthony G. Hay, Michael L. Shuler, He´ctor D. Abrun˜a, William C. Ghiorse, and Leonard W. Lion |
||||||||||||||
| Defective
presentation of the CD1d1-restricted natural Va14Ja18 NKT lymphocyte antigen
caused by -D-glucosylceramide synthase deficiency |
837 | A mast
cell-based biosensor has been developed to enable the use of these cells in
numerous applications including pharmaceutical screening, environmental monitoring, clinical diagnosis and homeland security. Rat basophilic leukemia (RBL) mast cells offer |
IgE receptors; Immunosensor; Bacterial diagnostics; Calcium responses; CD14 chimeric protein | For
confocal fluorescence experiments, 2.0mL of this cell suspension was plated into glass bottom Petri dishes (MatTek Corp. |
Biosensors and Bioelectronics | 23 | 2008 | February | 7 | P35G-1.5-14-F | RBL-2H3 | Theresa Curtis | Theresa Curtis a,∗, Rose Mary Z.G. Naal b,1, Carl Batt c, Joel Tabb a, David Holowka | |||||||||||||
| Design of
Mutant 2 Subunits as Decoy Molecules to Reduce the Expression of Functional Ca2 Channels in Cardiac Cells |
871 | To apply siRNA as a therapeutic agent, appropriate attention should be paid to the optimization of the siRNA gene silencing effect, both in terms of magnitude and duration. Intracellular time-controlled siRNA delivery could aid in tailoring the kinetics o | Biodegradable materials • Controlled release • Microgels • siRNA • Drug delivery • Biomedical applications | HUH7 cells were
seeded in sterile glass-bottomed culture dishes (MatTek Corporation, Ashland, MA) at a density of 3104 cells per cm2 and allowed to attach overnight. |
Advanced Functional Materials | 18 | 2008 | April | 7 | glass bottom dishes | HUH7 | Koen Raemdonck | Koen Raemdonck , Tinneke G. Van Thienen , Roosmarijn E. Vandenbroucke , Niek N. Sanders, Joseph Demeester , Stefaan C. De Smedt | |||||||||||||
| Determination
of Spatial Distributions of Zinc and Active Biomass in Microbial Biofilms by Two-Photon Laser Scanning Microscopy |
712 | Stargazin is an
accessory protein of AMPA receptors that enhances surface expression and also affects the biophysical properties of the receptor. AMPA receptor domains necessary for either of these two processes have not yet been identified. Here, we used |
Collagen- or
poly-Dlysine- coated 14-mm glass bottom culture dishes (MatTek Corp., Ashland, MA) were incubated with ECL Attachment Matrix (Upstate Cell Signaling Solutions, Lake Placid, NY) for 1 h at 37 °C then washed with cMEM before plating cells. |
Journal of Biological Chemistry | 281 | 2006 | August | 33 | P35GC-0-14-C | Human | Embryonic Kidney Fibroblasts | HEK-293 | Bedoukian, Matthew A. | Matthew A. Bedoukian, Autumn M. Weeks, and Kathryn M. Partin | Colorado State University | |||||||||||
| Development of a mast cell-based biosensor | 825 | Lysophosphatidic
acid (LPA) stimulates cells by activation of five Gprotein coupled receptors,
termed LPA1-5. The LPA1 receptor is the most widely expressed and is a major
regulator of cell migration. In this study, we show that phorbol ester (PMA)-induce |
HeLa cells
were plated onto 35mm glass bottom microwell dishes (MatTek Corp., Ashland, MA). |
Journal of Biological Chemistry | 283 | 2008 | February | 9 | HeLa | Urs, Nikhil M. | Nikhil M. Urs, Andrew P. Kowalczyk and Harish Radhakrishna | Georgia Institute of Technology, Emory University | ||||||||||||||
| Dextran Microgels for Time-Controlled Delivery of siRNA | 793 | Purpose:The
purpose of this study was to investigate the feasibility of using confocal
laser scanning microscopy together with specific fluorescent markers to
assess the distribution of cellular organelles in corneal epithelium of rat
in vivo. Methods:F |
cornea: basic science • mitochondria • imaging/image analysis: non-clinical | Animals (n=5) were then placed on a custom-designed heated microscope stage (to maintain body temperature) with the cornea facing glass-bottom multi-well plates (MatTek Corp., Ashland, MA). | P06G-1.5-20-F | Rat | Cornea | Epithelial | Bantseev, V. | V. Bantseev, D. McCanna, J.-Y. Driot, K. W. Ward and J. G. Sivak | University of Waterloo, Bausch&Lomb, Inc. | |||||||||||||||
| Different Domains of the AMPA Receptor Direct Stargazin-mediated Trafficking and Stargazin-mediated Modulation of Kinetics | 841 | Many cell
types, including neurons and epithelial cells, express a variety of
annexins. Although the overall function has only been partially unravelled, a dominant feature is the formation of two-dimensional assemblies under the plasma membrane in a calc |
Annexin, Calcium binding, FRAP, FRET, Translocation | For imaging
experiments, cells were plated in 35 mm MatTek chambers (Ashland, MA) and transfected with FuGENE 6 reagent (Roche, Mannheim, Germany) at 50% confluency. |
Experimental Cell Research | 314 | 2008 | March | 5 | 35-mm glass bottom dishes | N1E-115 | Tatsiana Skrahina | Tatsiana Skrahina1, Alen Piljić1, Carsten Schultz | |||||||||||||
| Different mechanisms regulate lysophosphatidic acid-dependent versus phorbol ester-dependent internalization of the LPA1 receptor | 799 | Two types of
short double-stranded RNA molecules, namely microRNAs (miRNAs) and short
interfering RNAs (siRNAs), have emerged recently as important regulators of gene expression. Although these molecules show similar sizes and structural features, the mec |
For 6-well
plates and MatTek chambers (MatTek Corporation, USA) cells were sown one day before transfection at a density of 1.5–3 · 105 cells/well in antibiotic-free media. |
Nucleic Acids Research | 34 | 2006 | March | 5 | 6-well plates | HeLa SS6 | Ohrt, Thomas | Thomas Ohrt, Dennis Merkle, Karin Birkenfeld, Christophe J. Echeverri and Petra Schwille | Dresden University of Technology, Cenix BioScience GmbH | |||||||||||||
| Distribution of Organelles Identified in Epithelium of Rat Corneas in vivo Using Confocal Laser Scanning Microscopy | 741 | Stimulation of
a mutant angiotensin type 1A receptor (DRYAAY) with angiotensin II (Ang
II) or of a wild-type receptor with an Ang II analog ([sarcosine1,Ile4,Ile8]Ang II) fails to activate classical heterotrimeric G protein signaling but does lead to rec |
One day after transfection, cells were split onto collagen-coated 35-mm plastic dishes with glass bottoms (MatTek, Ashland, MA) and cultured overnight at 37°C. |
PNAS | 100 | 2003 | September | 19 | 35-mm
collagen-coated glass bottom dishes plastic dishes with glass bottoms |
Human | Embryonic Kidney | HEK-293 | Wei, Huijun | Huijun Wei*,
Seungkirl Ahn*, Sudha K. Shenoy*, Sadashiva S. Karnik‡, La´ szlo´ Hunyady§,
Louis M. Luttrell, and Robert J. Lefkowitz* |
Duke University
Medical Center, Cleveland Clinic Foundation, Semmelweis University,
Geriatrics Research, Education, and Clinical Center, Durham Veterans Affairs Medical Center |
|||||||||||
| Heterogeneity
and timing of translocation and membrane-mediated assembly of different annexins |
873 | Transcription-coupled
repair (TCR) plays a key role in the repair of DNA lesions induced by bulky adducts and is initiated when the elongating RNA polymerase II (Pol II) stalls at DNA lesions. This is accompanied by alterations in Pol II activity and stab |
Transcription-coupled
repair, RNA Polymerase II, DNA lesions, Recycling, Living mammalian cells |
Cells were
cultured at 39°C in glass-bottomed dishes (MatTek Cultureware, Ashland, MA). |
Journal of Cell Science | 121 | 2008 | April | 8 | P35G-1.0-14-C | HeLa | Alexandre E. Escarguei | Alexandre E.
Escargueil, Virginie Poindessou, Daniele Grazziotin Soares, Alain
Sarasin, Peter R. Cook and Annette K. Larsen |
|||||||||||||
| In situ fluorescence analysis demonstrates active siRNA exclusion from the nucleus by Exportin 5 | 736 | Vascular
endothelial growth factor (VEGF) and its receptors have been implicated as
key factors in tumor angiogenesis that are up-regulated by hypoxia.Weevaluated the effects of DNA-binding small molecules on hypoxia-inducible transcription of VEGF. A syn |
gene regulation hypoxia-inducible factor polyamide | Incubations were
performed by adding 150 l of cells into culture dishes equipped with glass bottoms for direct imaging (MatTek, Ashland, MA). |
PNAS | 101 | 2004 | November | 48 | glass bottom
dishes equipped with glass bottoms for direct imaging |
Human | cervical epithelial adenocarcinoma cell | HeLa | Bogdan Z. | Bogdan Z.
Olenyuk*, Guo-Jun Zhang, Jeffery M. Klco, Nicholas G. Nickols*, William G.
Kaelin, Jr.‡, and Peter B. Dervan |
California Institute of Technology, Dana–Farber Cancer Institute, Harvard Medical School | ||||||||||
| Independent
-arrestin 2 and G protein-mediated pathways for angiotensin II activation of
extracellular signal-regulated kinases 1 and 2 |
906 | Protein kinase
Cs (PKCs) are important effectors of synaptic plasticity. In Aplysia, there
are two major phorbol ester-activated PKCs, Ca2-activated PKC Apl I and Ca2-independent PKC Apl II. Functional Apl II, but not Apl I, in sensory neurons is requir |
Synapses; synaptic transmission; synaptic plasticity; protein kinase C; Aplysia; learning | For
transfection, cells were plated on Mat- Tek glass bottom culture dishes (MatTek Corporation, Ashland, MA) with a glass surface of 14 mm and a coverslip thickness of 1.5 mm at a density of 0.11 106 cells/35 mm dish. |
Journal of Neuroscience | 26 | 2006 | August | 34 | P35G-1.5-14-C | SF9 | Yali Zhao | Yali Zhao, Karina Leal, Carole Abi-Farah, Kelsey C. Martin, Wayne S. Sossin, and Marc Klein | |||||||||||||
| Influence of
irofulven, a transcription-coupled repairspecific antitumor agent, on RNA polymerase activity, stability and dynamics in living mammalian cells |
765 | Rho GTPases are
signaling molecules known to control cell motility. Several recent studies
have suggested a role for Rho proteins in mediating tumor metastasis independent of their a¡ects on cell proliferation. As Rho proteins require post-translational m |
MDCK cells were plated on to 3 cm dishes with built-in coverslips (MatTek, Ashland, MA) | p35g-1.5-10-C | Dog | Kidney | MDCK | Collisson, Eric A. | Eric A. Collisson, Dafnis C. Carranza, Ingrid Y. Chen, and Michael S. Kolodney | UCLA School of Medicine, | ||||||||||||||||
| Inhibition of
vascular endothelial growth factor with a sequence-specific hypoxia
response element antagonist |
767 | The combination
of fluorescent genetically encoded proteins with mouse engineering provides a
fascinating means to study dynamic biological processes in mammals. At present, green fluorescent protein (GFP) mice were mainly developed to study gene expressi |
behavioral desensitization | green fluorescent protein | neuroanatomy | real-time imaging | receptor internalization | Cells were
seeded in glass-bottom, 32-mm diameter plastic dishes (MatTek) coated with polyL-lysine (Sigma). |
32-mm diameter plastic dishes | Mouse | Neurons, Hippocampus | Scherrer, Grégory | Grégory
Scherrer*, , Petra Tryoen-Tóth*, , Dominique Filliol*, Audrey Matifas*, Delphine Laustriat*, Yu Q. Cao , Allan I. Basbaum , Andrée Dierich*, Jean-Luc Vonesh*, Claire Gavériaux-Ruff*, and Brigitte L. Kieffer*, |
Centre National
de la Recherche Scientifique/Institut National de la Santé et de la Recherche Médicale/Université Louis Pasteur, Stanford University School of Medicine, University of California San Francisco |
||||||||||||||||
| Isoform
Specificity of PKC Translocation in Living Aplysia Sensory Neurons and a Role for Ca2-Dependent PKC APL I in the Induction of Intermediate-Term Facilitation |
891 | Although
lysophosphatidic acid (LPA) is known to increase intracellularfree calcium
concentration ([Ca2+]i) in different cell types, the effect of LPA on the skeletal muscle cells is not known. The present study was therefore undertaken to examine the eff |
Lysophosphatidic
acid • skeletal muscle cell • intracellular free calcium • receptor operated Ca2+-channels • sarcolemmal Na+-Ca2+ exchanger |
The cells were
cultured on the Glass Bottom Culture Dishes (MatTek, Ashland, MA, USA) |
Journal of Cellular and Molecular Medicine | 12 | 2008 | June | 3 | glass bottom dishes | C2C12 | Yan-Jun Xu | Yan-Jun Xu, Paramjit S. Tappia, Ramesh K. Goyal, Naranjan S. Dhalla | |||||||||||||
| Isoprenylation is Necessary for the Full Invasive Potential of RhoA Overexpression in Human Melanoma Cells | 847 | Charcot–Marie–Tooth
Type 2A is a dominantly inherited peripheral neuropathy characterized by
axonal degeneration of sensory and motor nerves. The disease is caused by mutations in the mitochondrial fusion gene MFN2. Mfn2 is an integral outer mitochondrial |
CMT2A; Peripheral neuropathy; Mitochondria; Mitofusin; Mfn2; Mfn1; GTPase; Membrane fusion; Fibroblasts | Approximately
10,000 cells per population were plated on 35 mm poly-D-lysine coated glass-bottom culture dishes (MatTek Corp.) |
Experimental Neurology | 211 | 2008 | May | 1 | glass bottom dishes | CMT2A | Elizabeth A. Amiott | Elizabeth A.
Amiott a, Paul Lott b,1, Jamie Soto c, Peter B. Kang d, J. Michael McCaffery
e, Salvatore DiMauro f, E. Dale Abel c, Kevin M. Flanigan b, Victoria H. Lawson b,⁎,2, Janet M. Shaw |
|||||||||||||
| Knockin mice
expressing fluorescent -opioid receptors uncover G proteincoupled receptor dynamics in vivo |
817 | Persistent reovirus infections of murine L929 cells select cellular mutations that inhibit viral disassembly within the endocytic pathway. Mutant cells support reovirus growth when infection is initiated with infectious subvirion particles (ISVPs), which | L cells and LX cells (105) were grown on 12-mm glass coverslips (Fisher Scientific, Pittsburgh, Pa.) or in glass-bottomed MatTek dishes (MatTek Corp., Ashland, Mass.) for 2 days. | Journal of Virology | 73 | 1999 | November | 11 | Mouse | L cells and LX cells | Baer, Geoffrey S. | Geoffrey S. Baer,, Daniel H. Ebert,, Chia J. Chung, Ann H. Erickson, and Terence S. Dermody | Vanderbilt University School of Medicine, University of North Carolina at Chapel Hill | |||||||||||||
| Mechanisms of
the lysophosphatidic acid-induced increase in [Ca2+]i in skeletal muscle cells |
779 | We
identify a human mutation (E1053K) in the ankyrin-binding motif of Nav1.5
that is associated with Brugada syndrome, a fatal cardiac arrhythmia caused by altered function of Nav1.5. The E1053K mutation abolishes binding of Nav1.5 to ankyrin-G, and also |
arrhythmia SCN5A targeting trafficking sudden cardiac death | After
washes, buffer was removed and the pellet was resuspended in Vectashield and plated onto Mattek 35-mm plates. |
PNAS | 101 | 2004 | December | 50 | P35G-1.5-14-C | Rat | Cardiomyocytes | Mohler, Peter J. | Peter J.
Mohler*‡, Ilaria Rivolta§, Carlo Napolitano§, Guy LeMaillet, Stephen Lambert,
Silvia G. Priori§, and Vann Bennett |
Duke University Medical Center, University of Pavia, University of Massachusetts Medical School | |||||||||||
| Mitochondrial
fusion and function in Charcot–Marie–Tooth type 2A patient fibroblasts with mitofusin 2 mutations |
694 | The secretion
of luteinizing hormone (LH) is cued by the hypothalamic neuropeptide, gonadotropin-releasing hormone (GnRH). Following delivery to the anterior pituitary gland via the hypothalamic-pituitary portal vasculature, GnRH binds to specific high af |
GnRH Mediated, Anterior Pituitary, Neuroendocrine Plasticity | Slices intended
for use in video microscopy were individually placed on glass-bottomed culture dishes (MatTek Corporation) that had been previously coated in poly-D-lysine and 8 collagen (Vitrogen 100, Cohesion). |
Endocrinology | 148 | 2007 | April | 4 | P35GC-0-14-C culture dishes | Mice (murine), Ewe | Pituitary | T3-1 | Navratil | Amy M. Navratil, J. Gabriel Knoll, Jennifer D. Whitesell, Stuart A. Tobet, Colin M. Clay | University of California San Diego, Colorado State University | ||||||||||
| Mutant Cells Selected during Persistent Reovirus Infection Do Not Express Mature Cathepsin L and Do Not Support Reovirus Disassembly | 863 | Mutations in the neurofibromatosis type 1 (NF1) tumor suppressor gene predispose individuals to a variety of benign and malignant tumors. Many tumor suppressors ‘shuttle’ between the nucleus and the cytoplasm, thus regulating their function. By expressing | Neurofibromatosis type 1; Neurofibromin; Nuclear localization signal; Nuclear localization; Alternative splicing | For live cell imaging and photobleaching experiments, cells were plated, transfected and observed in glass bottom culture dishes (MatTek Corporation) | FEBS Letters | 560 | 2004 | February | 1 | glass bottom dishes | COS-7 cells and PLC/PRF/5 cells | Ina Vandenbroucke | Ina Vandenbroucke, Patrick Van Oostveldt, Elisabeth Coene , Anne De Paepe and Ludwine Messiaen | |||||||||||||
| Nav1.5 E1053K
mutation causing Brugada syndrome blocks binding to ankyrin-G and expression
of Nav1.5 on the surface of cardiomyocytes |
910 | NF-kB
transcription factors are involved in the cellular response to stress, and are regulated by inhibitor (IkB) proteins, which prevent NF-kB-mediated transcription by maintaining NF-kB in the cytoplasm. Proteins from other pathways are also known to re |
NF-kB, STAT6,
Glucocorticoid receptor, Signal transduction, Fluorescent protein fusions, Confocal microscopy |
For confocal
microscopy, cells were plated on 35 mm Mattek dishes (Mattek, USA) at 2´104 cells per plate in 2 ml medium. |
Journal of Cell Science | 116 | 2003 | June | 12 | 35-mm glass bottom dishes | HeLa | Glyn Nelson | Glyn Nelson,
Geraint J. C. Wilde, David G. Spiller, Stephnie M. Kennedy, David W. Ray,
Elaine Sullivan, John F. Unitt and Michael R. H. White |
|||||||||||||
| Neuroendocrine Plasticity in the Anterior Pituitary: GnRH Mediated Movement in vitro and in vivo | 695 | Autoreactivity
in lupus requires the delivery of autoantigens to APCs in a proinflammatory
context. It has been proposed that apoptotic cells are a source of lupus autoantigens and targets for autoantibodies. Using a histone H2B-GFP fusion protein as trac |
Dendritic Cell
Phagocytosis, Nuclear Autoantigen Translocation, Autoantibody Opsonization, Presentation of Nuclear Antigens, Lupus |
K/H2B-GFP cells
were cultured in glass-bottom culture dishes (MatTek). Cells |
Journal of Immunology | 175 | 2005 | August | 4 | P35GC-0-14-C | Mice | bone marrow-derived DCs | K/H2B-GFP | Frisoni, Lorenza | Lorenza
Frisoni,* Lenese Mcphie,* Lucrezia Colonna, Uma Sriram, Marc
Monestier,‡ Stefania Gallucci, and Roberto Caricchio,* |
University of Pennsylvania, The Children’s Hospital of Philadelphia, Temple University School of Medicine | ||||||||||
| Neurofibromin is actively transported to the nucleus | 785 | A series of
hairpin pyrrole–imidazole polyamide–fluorescein conjugates were synthesized
and assayed for cellular localization. Thirteen cell lines, representing 11 human cancers, one human transformed kidney cell line, and one murine leukemia cell line, w |
Incubations were
performed by adding 150 l of cells into culture dishes equipped with glass bottoms for direct imaging (MatTek, |
PNAS | 100 | 2003 | October | 21 | P35G-1.0-14-C | Polyamides | Best, Timothy P. | Timothy P. Best*, Benjamin S. Edelson*, Nicholas G. Nickols, and Peter B. Dervan | California Institute of Technology | |||||||||||||
| NF-kB
signalling is inhibited by glucocorticoid receptor and STAT6 via distinct mechanisms |
828 | Polyethyleneimines
(PEIs) are efficient non-viral vectors for gene transfer. Heparan sulfate
proteoglycans have been proposed to be the cell-surface receptors for PEIDNA complexes (polyplexes). Here, we investigated if syndecan-1 (SDC1) and syndecan-2 (S |
SDC1-expressing
(a) or SDC2-expressing (b) cells were plated for 1 h on MatTek 3.5-mm Petri dishes. RITC-labeled polyplexes were then added to cells. |
Journal of Biological Chemistry | 283 | 2008 | March | 12 | P35GC-0-14-C | HEK293-EBNA1 cells | Se´bastien Paris | Se´bastien Paris, Alina Burlacu, and Yves Durocher | Biotechnology Research Institute, National Research Council Canada | |||||||||||||
| Nuclear Autoantigen Translocation and Autoantibody Opsonization Lead to Increased Dendritic Cell Phagocytosis and Presentation of Nuclear Antigens | 786 | MrgA1
and MrgC11 belong to a recently identified family of orphan G-protein coupled
receptors, called mrgs (mas-related genes). They are only expressed in a specific subset of sensory neurons that are known to detect painful stimuli. However, the precise |
MrgA1-GFP or
MrgC11-GFP stably expressing HEK293 cells were grown in 35-mm glass-bottomed dishes (Mat- Tek, Ashland,MA) inDMEMwith 10% FBS. |
P35G-0-14-C | mouse | embryonic fibroblasts | HEK293 | Han, Sang-Kyou | Sang-Kyou Han*, Xinzhong Dong‡, Jong-Ik Hwang*, Mark J. Zylka‡, David J. Anderson‡, and Melvin I. Simon | Howard Hughes Medical Institute, California Institute of Technology, | ||||||||||||||||
| Nuclear localization of pyrrole–imidazole polyamide–fluorescein conjugates in cell culture | 811 | Genome activity
and nuclear metabolism clearly depend on accessibility, but it is not known
whether and to what extent nuclear structures limit the mobility and access of individual molecules. We used fluorescently labeled streptavidin with a nuclear loca |
Mouse C2C12
myoblasts were cultured as described previously (19). For live cell analysis, cells were seeded onto either one-well LabTek chambers (Nalge Nunc International, Rochester, NY) or glass-bottom dishes (MatTek, Ashland, MA). |
Biophysical Journal | 94 | 2008 | April | 7 | P50G-1.5-14-F | Mouse | Myoblasts | C2C12 | Grunwald, David | David Gru¨
nwald,* Robert M. Martin,y Volker Buschmann,yz David P. Bazett-Jones,§
Heinrich Leonhardt,z Ulrich Kubitscheck,* and M. Cristina Cardoso |
Rheinische
Friedrich-Wilhelms-University, yMax Delbru¨ck Center for Molecular Medicine, Ludwig Maximilians University Munich, The Hospital for Sick Children, Toronto |
|||||||||||
| Opposing Roles of Syndecan-1 and Syndecan-2 in Polyethyleneimine-mediated Gene Delivery | 698 | Distinct
changes in glycogen synthase kinase-3 (GSK-3) signalling can regulate neuronal morphogenesis including the determination and maintenance of axonal identity, and are required for neurotrophin-mediated axon elongation. In addition, we have previous |
PI3K, GSK-3,
PTEN, Axon guidance, Semaphorin 3A, Cytoskeleton, Signal transduction |
For
live-cell imaging, nucleofected cells were cultured on poly-L-lysine (50 g/ml)/laminin (20 g/ml)-coated glass-bottom dishes (MatTek Corporation) for 15 hours. |
Journal of Cell Science | 119 | 2006 | March | 5 | poly-L-lysine
(50 g/ml)/laminin (20 g/ml)-coated glass-bottom dishes |
E18 rat | embryos | DRGs | Chadborn, Neil H. | Neil H.
Chadborn,*, Aminul I. Ahmed,*, Mark R. Holt, Rabinder Prinjha, Graham A.
Dunn, Gareth E. Jones and Britta J. Eickholt,‡ |
King’s College London, Guy’s Campus; GlaxoSmithKline | ||||||||||
| Orphan G protein-coupled receptors MrgA1 and MrgC11 are distinctively activated by RF-amide-related peptides through the Gq/11 pathway | 889 | Astrocytes play
an essential role in the maintenance and protection of the brain, which we
reported was diminished with age. Here, we demonstrate that activation of a purinergic receptor (P2Y-R) signaling pathway, in astrocytes, significantly increases th |
Intracellular Ca2; mitochondria; IP3 ; P2Y-R; two-photon; metabolism | Mouse cortical
neurons (#M-CX-300; Cambrex) were then cultured onto six-well mat-tek glass bottom plates and maintained in Neurobasal Medium |
Journal of Neuroscience | 27 | 2007 | June | 24 | 6-well glass bottom plates | Mouse cortical neurons | Jun Wu | Jun Wu, J. Deborah Holstein, Geeta Upadhyay, Da-Ting Lin, Stuart Conway, Elizabeth Muller , and James D. Lechleiter | |||||||||||||
| Probing Intranuclear Environments at the Single-Molecule Level | 855 | Specific
assembly of ribonucleoprotein complexes is essential in controlling various cellular functions including gene regulation. Diverse scaffolds containing proteins or nucleic acids could play key roles in stabilizing specific ribonucleoprotein comple |
human CyclinT1,
positive transcription elongation factor complex b, RNA–protein interactions, trans-activation responsive RNA, Tat protein |
Cells were
cultured on 35 mm plates with coverslip glass bottoms (MatTek Corporation, Ashland, MA, USA) |
Chemical Biology and Drug Design | 69 | 2007 | April | 4 | 35-mm glass bottom dishes | HeLa | Ya-Lin Chiu | Ya-Lin Chiu, Hong Cao and Tariq M. Rana | |||||||||||||
| PTEN couples Sema3A signalling to growth cone collapse | 809 | Signaling in
mast cells and basophils is mediated through IgE and its high affinity cell
surface receptor, FcRI. Crosslinking of the receptors by a cognate
multivalent antigen leads to degranulation and release of mediators of the allergic immune respons |
RBL mast cells,
quantitative microscopy, confocal image processing, GFP-labeled signaling proteins, co-localization analysis, Fc-epsilon-RI. |
The cells were
plated overnight in 35mm MatTek dishes (MatTek Corporation, Ashland, MA) at a subconfluent density ( 4 × 105 cells per dish) prior to transfection. |
Cell Biophysics | 94 | 2008 | June | 12 | P35G-1.5-14-C | RBL-2H3 | Das, Raibatak | Raibatak Das, Stephanie Hammond, David Holowka and Barbara Baird | Cornell University | ||||||||||||
| Purinergic
Receptor-Stimulated IP3-Mediated Ca2 Release Enhances Neuroprotection by Increasing Astrocyte Mitochondrial Metabolism during Aging |
775 | Many pathogenic
and symbiotic Gram-negative bacteria employ type III secretion systems to
inject ‘‘effector’’ proteins into eukaryotic host cells. These effectors manipulate signaling pathways to initiate symbiosis or disease. By using time-lapse microsco |
effector protein time-lapse microscopy | pGFP–InvB-transfected
Cos7 cells on glass-bottom dishes (MatTek, Ashland, MA) were transferred into Hank’s balanced salt solution (GIBCO) |
PNAS | 102 | 2005 | August | 35 | P35G-0-14-C | Cos7 | Schlumberger, Markus C. | Markus C.
Schlumberger, Andreas J. Mu¨ ller, Kristin Ehrbar, Brit Winnen, Iwan Duss,
Ba¨ rbel Stecher, and Wolf-Dietrich Hardt |
Eidgeno¨ ssische Technische Hochschule | ||||||||||||
| Quantitative Analysis of RNA-mediated Protein–Protein Interactions in Living Cells by FRET | 769 | The rapid
growth and poor vascularization of solid tumors expose cancer cells to
hypoxia, which promotes the metastatic phenotype by reducing intercellular adhesion and increasing cell motility and invasiveness. In this study, we found that hypoxia increa |
NADH E-cadherin metastasis HIF1 adhesion | Cells were grown
for 2 days on glass 35-mm dishes (MatTek, Ashland, 9032 www.pnas.orgcgidoi10.1073pnas.0603269103 Zhang et al. MA) and maintained at 37°C and 5% CO2 in a humidified chamber on the microscope stage (Zeiss). |
PNAS | 103 | 2006 | June | 24 | P35G-0-14-C | Human | non-small-cell lung cancer, colon carcinoma | H1299, HT29 | Zhang, Qinghong | Qinghong Zhang*,
Su-Yan Wang*, Amanda C. Nottke*, Jonathan V. Rocheleau‡, David W.
Piston‡, and Richard H. Goodman |
Oregon Health & Sciences University, Vanderbilt University | ||||||||||
| Real-time
cross-correlation image analysis of early events in IgE receptor signaling. |
791 | The regulation
of intracellular localization of AFX, a human Forkhead transcription factor,
was studied. AFX was recovered as a phosphoprotein from transfected COS-7 cells growing in the presence of FBS, and the phosphorylation was eliminated by wortmanni |
CHO-K1 cells
transfected with AFX-GFP or its mutants were spread onto glass-bottom culture dishes (MatTek, Ashland, MA) and maintained for at least 16 h before observation. |
glass-bottom
culture dishes |
Hamster | Ovary | CHO-K1 | Takaishi, Hiroshi | Hiroshi
Takaishi*, Hiroaki Konishi*, Hidenori Matsuzaki*, Yoshitaka Ono, Yasuhito
Shirai*, Naoaki Saito*, Tadahiro Kitamura‡, Wataru Ogawa‡, Masato Kasuga‡, Ushio Kikkawa*§, and Yasutomi Nishizuka |
Kobe University, Kobe University School of Medicine | ||||||||||||||||
| Real-time imaging of type III secretion: Salmonella SipA injection into host cells | 792 | The G
protein-coupled m-opioid receptor (mOR) mediates the physiological effects of
endogenous opioid peptides as well as the structurally distinct opioid
alkaloids morphine and etorphine. An intriguing feature of mOR signaling is the differential recepto |
HEK 293 cells
were transfected to overexpress mOR and low levels of barr2yGFP with or without GRK2 or GRK2-K220M, and were plated on 35-mm glass-bottomed culture dishes (MatTek, Ashland, MA) |
35-mm glass bottom culture dishes | HEK 293 | Zhang, Jie | JIE ZHANG*,
STEPHEN S. G. FERGUSON*, LARRY S. BARAK*, SOBHA R. BODDULURI*, STE´PHANE A.
LAPORTE*, PING-YEE LAW‡, AND MARC G. CARON |
Duke University Medical Center, University of Minnesota Medical School | ||||||||||||||||||
| Redox sensor CtBP mediates hypoxia-induced tumor cell migration | 795 | Protein kinase
D2 (PKD2) belongs to the PKD family of serine/threonine kinases that is
activated by phorbol esters and G protein-coupled receptors (GPCRs). Its C-terminal regulatory domain comprises two cysteine-rich domains (C1a/C1b) followed by a plecks |
For live cell
imaging cells were grown and transfected in MatTek glass-bottomed
dishes (MatTek, Ashland, MA). |
Molecular Biology of the Cell | 16 | 2005 | September | 9 | glass bottom dishes | Human | Kidney | Embryonic | HEK-293 | Auer, Alexandra | Alexandra Auer,*
Julia von Blume,* Sabine Sturany,*‡ Go¨ tz von Wichert,* Johan Van Lint,§ Jackie Vandenheede,§ Guido Adler,* and Thomas Seufferlein |
Medical University of Ulm, Katholieke Universiteit Leuven | ||||||||||
| Regulation of nuclear translocation of Forkhead transcription factor AFX by protein kinase B | 703 | Experiments
with isolated mitochondria have established that these organelles are
pivotal intracellular sources of superoxide in a variety of pathophysiological conditions. Recently, a novel fluoroprobe MitoSOX Red was introduced for selective detection o |
Superoxide,
Mitochondria, Free radicals, MitoSOX, Antimycin A (AntA), Paraquat (PQ),
Doxorubicin (DOX), Cardiomyocytes, H9c2, Human coronary artery endothelial cells (HCAECs) |
Cells
were cultured in 12 well cell culture plates or glass bottom dishes (MatTek, Ashland, MA) with DMEM (Gibco, Invitrogen, Carlsbad, CA) containing 10% fetal bovine serum (Invitrogen, Carlsbad, CA), 100 U/ml penicillin and 100 μg/ml streptomycin at 37 |
Biochemical and Biophysical Research Communications | 358 | 2007 | June | 1 | P35GC-1.0-14-C, P DCF OS 30 | Rat | embryonic ventricular myocardial | H9c2 | Mukhopadhyaya, Partha | Partha Mukhopadhyay,a Mohanraj Rajesh,a Kashiwaya Yoshihiro,b György Haskó, and Pál Pachera* | National Institutes of Health/NIAAA, UMDNJ-New Jersey Medical School | ||||||||||
| Role for G
protein-coupled receptor kinase in agonist-specific regulation of m-opioid receptor responsiveness |
821 | Patients on a
statin regimen are at a decreased risk of death due to bacterial sepsis. We
have found that protection by simvastatin includes the inhibition of host
cell invasion by Staphylococcus aureus, the most common etiologic agent of sepsis. Inhibiti |
For confocal
imaging, cells were plated as above on 35 mm glass-bottom dishes (MatTek, Ashland, MA) coated as above. |
Journal of Pharmacology And Experimental Therapeutics | 326 | 2008 | July | 1 | P35GC-1.5-10-C | Human | Umbilical Cord | Endothelial | HUVEC | Horn, Mary P. | Mary P. Horn,
Sharmon M. Knecht, Frances L. Rushing, Julie Birdsong, C. Parker Siddall, Charron M. Johnson, Terri N. Abraham, Amy Brown, Catherine B. Volk, Kelly Gammon, Derron L. Bishop, John L. McKillip, and Susan A. McDowell |
Ball State University, Indiana School of Medicine | ||||||||||
| Role of the
Regulatory Domain of Protein Kinase D2 in Phorbol Ester Binding, Catalytic Activity, and Nucleocytoplasmic Shuttling |
745 | Exocytic
vesicle fusion requires both the SNARE family of fusion proteins and a
closely associated regulatory subunit of the Sec1/ Munc18 (SM) family. In principle, SM proteins could act at an early SNARE assembly step to promote vesicle–plasma membrane a |
giant liposomes Sec1/Munc18 | Samples were prepared in glass-bottom dishes (P35G-1.5-14-C; MatTek) coated with 10% (wt/vol)BSAfor1hatroomtemperature |
PNAS | 105 | 2008 | February | 7 | P35G-1.5-14-C | Rat | Munc18-1 | Tareste, David | David Tareste, Jingshi Shen, Thomas J. Melia, and James E. Rothman | Columbia University | |||||||||||
| Simple quantitative detection of mitochondrial superoxide production in live cells | 883 | The
pseudorabies virus (PRV) Us9 protein plays a central role in targeting viral
capsids and glycoproteins to axons of dissociated sympathetic neurons. As a result, Us9 null mutants are defective in anterograde transmission of infection in vivo. However, |
Pseudorabies Virus, Viral Us9 Protein, Lipid Rafts, PRV, NGF | PC12 cells were
cultured on MatTek Corp. glass-bottomed dishes. |
PLoS Pathogens | 4 | 2008 | May | 5 | glass bottom dishes | PC12 | Mathew G. Lyman | Mathew G. Lyman, Dusica Curanovic, Lynn W. Enquist | |||||||||||||
| Simvastatin inhibits Staphylococcus aureus host cell invasion through modulation of isoprenoid intermediates | 850 | Although
Candida containing biofilms contribute to the development of oral candidosis,
the characteristics of multi-species Candida biofilms and how oral bacteria modulate these biofilms is poorly understood. The aim of this study was to investigate inter |
Candida albicans, Candida glabrata, Dual-species biofilm, Streptococcus mutans, Confocal scanning laser microscopy | The discs were
carefully removed from the wells (after 24 h of biofilm formation), placed (face down) on a 35- mm-diameter glass-bottom Petri-dish (MatTek Corp., Ashland, MS, USA) |
Archives of Oral Biology | 2008 | P35GC-1.5-10-C | sHA, sPMMA and sSL discs |
Tatiana Pereira-Cenci | Tatiana
Pereira-Cenci a,b, Dong Mei Deng b, Eefje Anne Kraneveld b, Erik Martinus Marie Manders c, Altair Antoninha Del Bel Cury a, Jacob Martien ten Cate b, Wim Crielaard |
||||||||||||||||
| SNAREpin/Munc18 promotes adhesion and fusion of large vesicles to giant membranes | 878 | Spatial
organisation of nuclear compartments is an important regulator of chromatin function, yet the molecular principles that maintain nuclear architecture remain ill-defined. We have used RNA interference to deplete key structural nuclear proteins, the |
Nuclear lamin
proteins, Nucleoskeleton, Nuclear organisation, RNA synthesis, Chromosome territories |
For live imaging
of CTs, labelled cells were grown on 35 mm Petri dishes with 13 mm glass inserts (MatTek Corporation) in medium without Phenol Red. |
Journal of Cell Science | 121 | 2008 | April | 7 | 35-mm glass bottom dishes | HeLa | Chi W. Tang | Chi W. Tang,
Apolinar Maya-Mendoza, Catherine Martin, Kang Zeng, Songbi Chen, Dorota
Feret, Stuart A. Wilson and Dean A. Jackson |
|||||||||||||
| Targeting of
Pseudorabies Virus Structural Proteins to Axons Requires Association of the Viral Us9 Protein with Lipid Rafts |
834 | During
vertebrate cell division, chromosomes oscillate with periods of smooth motion interrupted by abrupt reversals in direction. These oscillations must be spatially constrained in order to align and segregate chromosomes with high fidelity, but the mol |
Kinesin-8 Motor
Kif18A, Kinetochore Movements, MitoticChromosomeAlignment |
HeLa cells were
cultured in MEMalpha (Life Technologies) medium with 10% FBS (Hyclone) at 37C and 5% CO2 on 35 mm2 glass coverslip dishes coated with poly-l-lysine (MatTek |
Developmental Cell | 14 | 2008 | February | glass bottom dishes | HeLa | Jason Stumpff | Jason Stumpff,1 George von Dassow,2 Michael Wagenbach,1 Charles Asbury,1 and Linda Wordeman | ||||||||||||||
| The effect of
Streptococcus mutans and Candida glabrata on Candida albicans biofilms formed on different surfaces |
776 | All auxiliary
2 subunits of voltage-gated Ca2 (CaV) channels contain an
extracellular Von Willebrand factor-A (VWA) domain that, in 2-1 and -2, has a perfect metal-ion-dependent adhesion site (MIDAS). Modeling of the 2-2 VWA domain shows it to be high |
integrin neuron motif expression | For imaging GFP in live cells, glass-bottomed dishes (MatTek, Ashland, MA) were mounted in a humidified chamber on the microscope and perfused with 5% CO2. | PNAS | 102 | 2005 | August | 32 | glass bottom dishes | Cos7 | Canti, M. | C. Cantı´*,
M. Nieto-Rostro*, I. Foucault*, F. Heblich*, J. Wratten, M. W. Richards, J.
Hendrich, L. Douglas, K. M. Page, A. Davies, and A. C. Dolphin |
University College London | ||||||||||||
| The integrity
of a lamin-B1-dependent nucleoskeleton is a fundamental determinant of RNA synthesis in human cells |
807 | We demonstrate
that physiologically relevant perturbations in the osmotic environment
rheostatically regulate a gatekeeping function for the nucleolus that controls nucleolin’s spatial dynamics and functions. HeLa cells and U2-OS osteosarcoma cells were o |
One day prior
to transfection with MINPP, cells were seeded onto 35mm dish with 14mm microwell (MatTek) at a density of 8 x 105 cells/dish |
P35G-1.5-14-C | HeLa and H1299 | Yang, Ling | Ling Yang , Jeff M. Reece , Jaiesoon Cho ,, Carl D. Bortner 4, and Stephen B. Shears | National
Institute of Environmental Health Sciences, NIH, Reecent Technologies, Konkuk University |
||||||||||||||||||
| The Kinesin-8
Motor Kif18A Suppresses Kinetochore Movements to ControlMitoticChromosomeAlignment |
729 | Animals use
their gustatory systems to evaluate the nutritious value, toxicity, sodium content, and acidity of food. Although characterization of molecular identities that receive taste chemicals is essential, molecular receptors underlying sour taste sen |
chemical senses polycystic kidney disease gustation ion channel acid | For calcium
imaging, cells were seeded on glass-bottom plates with poly-D-lysine-coated coverslips (Mat- Tek) and plasmidDNAwas transfected with Lipofectamine 2000 (Invitrogen) and incubated for 30–42 h before dye loading. |
glass-bottom plates with poly-D-lysine-coated coverslips | Rabbit | Tongue | PKD1L3 and PKD2L1 | Ishimaru, Yoshiro | Yoshiro Ishimaru*, Hitoshi Inada, Momoka Kubota*, Hanyi Zhuang*, Makoto Tominaga‡, and Hiroaki Matsunami | Duke University Medical Center, National Institutes of Natural Sciences, Graduate University for Advanced Studies, Okazaki, Japan | |||||||||||||||
| The
metal-ion-dependent adhesion site in the Von Willebrand factor-A domain of
2 subunits is key to trafficking voltage-gated Ca2 channels |
849 | Various
molecular mechanisms of unconventional secretion of fibroblast growth factor 2 and galectin-1 have been proposed. A non-vesicular pathway that is based on direct translocation across the plasma membrane has been described. In other studies, howeve |
Unconventional
protein secretion; Non-classical export; Fibroblast growth factor 2; Galectin-1 |
CHO cells were
grown on glass coverslips (MatTek Corp., Ashland, USA), processed for live cell imaging and viewed with a Zeiss LSM 510 confocal microscope. |
FEBS Letters | 582 | 2008 | March | 9 | glass coverslips | CHO | Claudia Seelenmeyer | Claudia Seelenmeyer1, Carolin Stegmayer1, Walter Nickel | |||||||||||||
| THE NUCLEOLUS EXHIBITS AN OSMOTICALLY-REGULATED GATEKEEPING ACTIVITY THAT CONTROLS NUCLEOLIN’S SPATIAL DYNAMICS AND FUNCTIONS. | 872 | The characteristic bipolar shape of the mitotic spindle is produced by the focusing of the minus ends of microtubules at the spindle poles. The focus is maintained by the centrosome, a microtubule-nucleating organelle, as well as by proteins that are capa | RNAi, Centrosome, Microtubule, Kinetochore, -tubulin | Cells were adhered on Con-A treated glass-bottom culture dishes (35-mm, MatTek) | Journal of Cell Science | 121 | 2008 | April | 8 | 35-mm glass bottom dishes | Drosophila Schneider cells (S2 | Matthew A. Trammell | Matthew A. Trammell, Nicole M. Mahoney, David A. Agard and Ronald D. Vale | |||||||||||||
| Transient receptor potential family members PKD1L3 and PKD2L1 form a candidate sour taste receptor | 826 | Antigen-mediated
cross-linking of the high affinity receptor for IgE (FcRI), in the plasma
membrane of mast cells, is the first step in the allergic immune response. This event triggers the phosphorylation of specific tyrosines in the cytoplasmic segments |
Adherent cells
(1.5 105 cells/ml) were plated onto glass-bottomed 35-mm Petri dishes (MatTek) and sensitized overnight |
Journal of Biological Chemistry | 283 | 2008 | March | 11 | P35G-1.5-14-C | Mast cell | RBL-2H3 | Davey | Angel M. Davey‡, Keith M. Krise‡, Erin D. Sheets‡§, and Ahmed A. Heikal | The Huck
Institutes of the Life Sciences, Pennsylvania State University |
||||||||||||
| Unconventional
secretion of fibroblast growth factor 2 and galectin-1 does not require shedding of plasma membrane-derived vesicles |
909 | Close homolog
of L1 (CHL1) is a member of the L1 family of cell adhesion molecules expressed by subpopulations of neurons and glia in the central and peripheral nervous system. It promotes neurite outgrowth and neuronal survival in vitro. This study descr |
After washing in
ice-cold HBSS, the cells were resuspended in 10% fetal bovine serum in DMEM, plated onto collagen-coated MatTek plates (MatTek Corp., Ashland, MA) |
Journal of Biological Chemistry | 278 | 2003 | July | 27 | collagen-coated plates | HEK293 | Mona Buhusi, | Mona Buhusi,
Bentley R. Midkiff, Amanda M. Gates, Melanie Richter, Melitta
Schachner, and Patricia F. Maness |
||||||||||||||
| vMob4 plays a role in spindle focusing in Drosophila S2 cells | 896 | Microtubule-associated
protein 2 (MAP2) is a neuronal phosphoprotein that promotes net microtubule growth and actin cross-linking and bundling in vitro. Little is known about MAP2 regulation or its interaction with the cytoskeleton in vivo. Here we invest |
Samples for
live-cell microscopy were seeded onto 35-mm culture dishes with a glass coverslip mounted over a hole in the bottom of the dish (Mattek Corp., Ashland, MA). |
Molecular Biology of the Cell | 11 | 2000 | October | 10 | 35-mm glass bottom dishes | HeLa | Rachel S. Ozer | Rachel S. Ozer, and Shelley Halpain | ||||||||||||||
| 4-D Single Particle Tracking Of Synthetic And Proteinaceous Microspheres Reveals Preferential Movement Of Nuclear Particles Along Chromatin – Poor Tracks | 306 | The dynamics of nuclear organization, nuclear bodies and RNPs in particular has been the focus of many studies. To understand their function, knowledge of their spatial nuclear position and temporal translocation is essential. Typically, such studies gene | SW13 cells were cultured in P35G-1.5-7-C-Grid cell locate culture dishes (MatTek Corporation, Ashland, MA) in complete DMEM medium for 1 day after plating. | BioMed Central- Cell Biology | 5 | 2004 | November | 45 | P35G-1.5-7-CGRID | HUMAN | ADRENAL CORTEX | IZSBS BS TCL 62 Morphology: epithelial-like Species: human, Caucasian female 55 years old; Tissue: adrenal cortex; Tumor: adenocarcinoma; Validated by isoenzymes: confirmed as human with NP, AST, LD Depositor: obtained from ATCC Properties: Susceptible to |
SW13 | Bacher, C. | Bacher, C., Reichenzeller, M., Athale, , C., Herrmann, H., and Eils, R. Division of Theoretical Bioinformatics, Deutsches Krebsforschungszentrum,Heidelberg, Germany. Division of Cell Biology, Deutsches Krebsforschungszentrum, Heidelberg, Germany. | Deutsches Krebsforschungszentrum | ||||||||||
| 4-Hydroxynonenal Induces Oxidative Stress And Death of Cultured Spinal Cord Neurons | 570 | Primary spinal
cord trauma can trigger a cascade of secondary processes leading to delayed
and amplified injury to spinal cord neurons. Release of fatty acids, in particular arachidonic acid, from cell membranes is believed to contribute significantly |
Arachidonic
acid, Lipid peroxidation, Oxidative stress, Spinal cord trauma, N-Acetylcysteine, Ebselen |
Immunocytochemistry
was performed to measure HNE formation in cultured spinal cord neurons exposed to arachidonic acid according to the procedure described earlier (Herbst et al., 1999). In brief, cultures of spinal cord neurons were prepared on glass |
spinal | MYOCYTES | Malecki | Andrzej Malecki,
Rosario Garrido, Mark P. Mattson, Bernhard Hennig, and Michal Toborek |
University of Kentucky | |||||||||||||||||
| A Carboxyl Leucine-Rich Region of Parathyroid Hormone- Related Protein is Critical for Nuclear Export | 579 | Parathyroid hormone-related protein is an oncofetal protein with distinct proliferative and anti-apoptotic roles that are affected by nucleocytoplasmic shuttling. The protein’s nuclear export is sensitive to leptomycin B, consistent with a CRM1 dependent | consensus sequence, confocal microscopy, exportin 1, fluorescence recovery after photobleaching, leptomycin, nucleocytoplasmic transport proteins, peptide hormones | To assess steady state cellular distributions of PTHrP, 293 cells were plated in 35-mm glass bottom culture dishes (MatTek, Ashland, MA) and transfected with GFP3-PTHrP chimeras. | Endocrinology | 147 | 2006 | February | 2 | HEK-293 | Pache, Jared C. | Jared C. Pache, Douglas W. Burton, Leonard J. Deftos, and Randolph H. Hastings | UCSD | |||||||||||||
| A Comparison Of The Sensitivity Of Photodamage Assays In Rat Basophilic Leukemia Cells | 358 | Many aspects of cellular function or physiology can be used to indicate the level of damage resulting from the application of potentially deleterious agents such as drugs, solvents or even light. The dose required to reach a specific biological endpoint w | photodamage assays, type I photooxidation reactions | RBL-2H3 cells
were grown in minimal essential medium (MEM-a) supplemented with 20%
fetal bovine serum and 100 units of penicillin-streptomycin. The 7mmgrid dishes were used for the experiments using the BrdU incorporation assay. The 14 mm dishes were |
Photochemistry and Photobiology | 81 | 2005 | May | 3 | P35G-1.5-7-CGRID | RAT | Egg chambers | Barth, Erin E. | Erin E. Barth, Richard Hallworth and Michael G. Nichols | Creighton University | |||||||||||
| A Novel Form Of Cellular Communication Among Thymic Epithelial Cells: Intercellular Calcium Wave Propagation | 309 | We here describe intercellular calcium waves as a novel form of cellular communication among thymic epithelial cells. We first characterized the mechanical induction of intercellular calcium waves in different thymic epithelial cell preparations: cortical | gap junctions, connexin43, P2 receptors • intercellular communication | "The typically heavier TNCs were allowed to sediment. TNCs obtained by this process were cultured for RNA extraction or, alternatively, plated in MatTek (Ashland, MA) imaging dishes for calcium wave experiments." | American Journal of Physiology- Cell Physiology | 285 | 2003 | November | 5 | THYMUS | TNC | Nihei, O. | Nihei,,, O., Campos de Carvalho, A., Spray, D., Savino,,4 W., and Alves L., Laboratory on Thymus Research, Department of Immunology, Institute Oswaldo Cruz, The Oswaldo Cruz Foundation, Rio de Janeiro; Institute of Biophysics, Federal University | The Oswaldo Cruz Foundation | ||||||||||||
| Activation Of Rac1 By A Crk Sh3-Binding Protein, Dock180 | 543 | DOCK180 is
involved in integrin signaling through CrkII-p130Cas complexes. We have studied the involvement of DOCK180 in Rac1 signaling cascades. DOCK180 activated JNK in a manner dependent on Rac1, Cdc42Hs, and SEK, and overexpression of DOCK180 inc |
DOCK180, actin cytoskeleton, Rho-family proteins, CrkII | NIH-3T3 cells
were plated subconfluently on fibronectin-coated glass dishes (Mat Tek) 16 hr before mincroinjection. Expression plasmids were injected into the nucleus by use of an Automated Microinjection System (Carl Zeiss). After 16 hr, cells were fi |
Genes & Development | 12 | 1998 | November | 21 | MOUSE, SWISS ALBINO | EMBRYO | ECACC 93061524 Morphology: Fibroblast Mouse Swiss NIH embryo contact inhibited Depositor: Obtained from ATCC, USA No restrictions. Patent: None Specified By Depositor Properties: Applications: DNA transfection studies |
FIBROBLAST | NIH-3T3 | Kiyokawa, Etsuko | Etsuko Kiyokawa,
Yuko Hashimoto, Shin Kobayashi, Haruhiko Sugimura, Takeshi Kurata, and Michiyuki Matsuda |
National Institute of Infectious Diseases | |||||||||
| Agonist- And Protein Kinase C-Induced Phosphorylation Have Similar Functional Consequences For Gastrin-Releasing Peptide Receptor Signaling Via Gq | 470 | Acute
desensitization of many guanine nucleotide-binding protein- coupled receptors (GPCRs) requires receptor phosphorylation and subsequent binding of an arrestin. GPCRs are substrates for phosphorylation by several classes of kinases. Gastrin-releas |
guanine
nucleotide-binding protein- coupled receptors, phosphorylation, carboxyl terminal domain, Acute desensitization, rhodopsin |
Stably
transfected Balb 3T3 fibroblasts (expressing high levels of GRPr constructs) were plated in MatTek glass-bottomed 35-mm dishes at 150 to 200,000 cells/dish. The next day, each dish was transfected with 0.1 to 1 g of EGFP-N1- - arrestin1 (arr2) |
Molecular Pharmacology | 64 | 2003 | October | 4 | MOUSE, SWISS ALBINO | NIH-3T3 | Ally, Roxanne A. | ROXANNE A. ALLY,
KIRK L. IVES, ELIE TRAUBE, IMAN ELTOUNSI, PEI-WEN CHEN, PATRICK J.
CAHILL, JAMES F. BATTEY, MARK R. HELLMICH, and GLENN S. KROOG |
Albert Einstein College of Medicine | ||||||||||||
| Alpha2-adrenergic agonist enrichment of spinophilin at the cell surface involves βγ subunits of Gi proteins and is preferentially induced by the α2A-subtype | 610 | Agonist activation regulates reciprocal interactions of spinophilin and arrestin with the α2A- and α2B-adrenergic receptor (AR) subtypes via their 3i loop. Since arrestin association with GPCR is preceded by redistribution of arrestin to the cell surface, | agonist activation, spinophilin, arrestin, βARK-C tail | MatTek dishes were purchased from MatTek Corporation (Ashland, MA). | Molecular Pharmacology | 67 | 2005 | May | 5 | HEK-293 | Brady, Ashley E. | Ashley E. Brady, Qin Wang, Patrick B. Allen, Mark Rizzo, Paul Greengard, Lee E. Limbird | Vanderbilt University | |||||||||||||
| Alterations In Receptor Expression Or Agonist Concentration Change The Pathways Gastrin-Releasing Peptide Receptoruses To Regulate Extracellular Signal-Regulated Kinase | 532 | G
protein-coupled receptors activate extracellular signalregulated kinases (ERKs) via different pathways in different cell types. In this study, we demonstrate that gastrin-releasing peptide receptor (GRPr) regulates ERK through multiple pathways in a |
Receptor
Expression, Agonist Concentration, Gastrin-Releasing Peptide Receptor,
extracellular signalregulated kinases, Mitogen-activated protein kinases, |
Myc-GRPr cells
were plated in glass bottom 35-mm dishes (Mat- Tek, Ashland, MA) at 150,000 cells/dish and cultured overnight. Cells were transfected with 0.3 g of pEGFP-N1-arrestin2 or arrestin3- GFP by LipofectAMINE method following the manufacturer’ |
Molecular Pharmacology | 66 | 2004 | December | 6 | Myc-GRPr | Chen, Pei-Wen | Pei-Wen Chen and Glenn S. Kroog | Albert Einstein College of Medicine | |||||||||||||
| Analysis Of Dna Fragmentation Of In Vitro Cultured Bovine Blastocysts Using Tunel | 233 | Programmed cell death (apoptosis) characteristically affects the single cells of blastocysts whereas necrosis affects cluster of cells in both the inner cell mass (ICM) and the trophectoderm (TE). This study uses the trophectodermrminal deoxynucleotidyl t | Apoptosis; Blastocysts; Bovine; Parthenogenotes; TUNEL | Prior to the live observations the parthenogenotes and the fertilized embryos were placed in a glass-bottom chamber (MatTek Corp., Ashland, MA) filled with culture medium and overlaid with pre-warmed mineral oil. | Theriogenology | 57 | 2002 | June | 9 | BOVINE | EMBRYO | Neuber, E. | Neuber,E., Luetjens, C.M., Chan, A. W. S. and Schatten, G. P. Oregon Regional Primate Research Center, Oregon Health Sciences University, Beaverton, USA. Theriogenology, 57, (9), 9-0, (00). | Oregon Health and Sciences University | ||||||||||||
| Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1) | 582 | Themammalianabasic-endonuclease1/redox-factor1 (APE1/Ref1) is an essential protein whose subcellular distributiondependsonthe cellular physiological status. However, its nuclear localization signals have not been studied in detail. We examined nuclear tra | abasic-endonuclease1/redox-factor1 (APE1/Ref1), reactive oxygen species, gene regulation, nuclear transport, Karyopherin a, amino acid | For live cell analysis, cells spread on glass-coated 35 mm dishes (Mattek) were transfected with DNA as described above, and then examined without fixation. | mouse | fibroblast-like | 3T3 | Jackson, Elias B. | Elias B. Jackson, Corey A. Theriot, Ranajoy Chattopadhyay, Sankar Mitra and Tadahide Izumi | University of Texas | ||||||||||||||||
| Analysis Of The Zebrafish Perplexed Mutation Reveals Tissue-Specific Roles For De Novo Pyrimidine Synthesis During Development | 355 | The zebrafish perplexed mutation disrupts cell proliferation and differentiation during retinal development. In addition, growth and morphogenesis of the tectum, jaw, and pectoral fins are also affected. Positional cloning was used to identify a mutation | cell proliferation, differentiation, retinal development, progenitor cells, mitosis, ethylnitrosourea, carbamoyl-phosphate synthetase2-aspartate transcarbamylase-dihydroorotase (CAD) | Stock solutions made in embryo medium and 50 nl of various concentrations were injected into the yolk sack of developing wild-type and perplexed embryos at 20 hr postfertilization (hpf). Partial rescue of eye, jaw, and fin development was observed at a mi | Genetics | 170 | 2005 | August | 4 | P35G-1.5-10-C | zebrafish | neuroepithelial | EMBRYO MEDIUM | Willer, G. B. | G. B. Willer, V. M. Lee, R. G. Gregg and B. A. Link | University of Louisville | ||||||||||
| Anesthetic-Induced Alteration Of Ca2+ Homeostasis In Neural Cells: A Temperature-Sensitive Process That Is Enhanced By Blockade Of Plasma Membrane Ca2+-Atpase Isoforms[Laboratory Investigations] | 450 | Many inhalation anesthetics at clinically relevant concentrations inhibit plasma membrane Ca2+-adenosine triphosphatase (PMCA) ion pumping in brain synaptic membranes and in cultured cells of neural origin. In this study, the authors investigated the effe | anesthetics, cytosolic calcium homeostasis, calcium, interneuronal signaling | Calcium imaging was done in the Vanderbilt University Cell Imaging Resource. A laser scanning confocal microscope (Zeiss LSM410, Seiles Instruments, St. Louis, MO), with diffraction-limited focusing of a laser beam, coupled with a special filter placed "c | Anesthesiology | 89 | 1998 | July | 1 | Rat | Pheochromocytoma | Franks, John J. | Franks, John J. MD; Wamil, Artur W. MD, PhD; Janicki, Piotr K. MD, PhD; Horn, Jean-Louis MD; Franks, William T. BA; Janson, Victoria E. BS, MBA; Vanaman, Thomas C. PhD; Brandt, Paul C. PhD | Vanderbilt University | ||||||||||||
| Apical And Basolateral Endocytic Pathways Of Mdck Cells Meet In Acidic Common Endosomes Distinct From A Nearly-Neutral Apical Recycling Endosome | 253 | Quantitative confocal microscopic analyses of living, polarized MDCK cells demonstrate different pH profiles for apical and basolateral endocytic pathways, despite a rapid and extensive intersection between the two. Three-dimensional characterizations of | acidification, endocytosis, endosome, epithelia, immunoglobulin A, MDCK, polarity, polymeric Ig receptor, transferring, transcytosis | After removing the legs of the filter units, living or fixed cells were observed by placing the entire filter unit on two 50µ m tape spacers attached to the coverslip of a coverslip-bottomed 35 mm dish (Mattek, Ashland, MA) mounted on the stage of an inve | Traffic | 1 | 2000 | June | 6 | DOG, COCKER SPANIEL FEMALE | KIDNEY | IZSBS BS CL 64 Morphology: epithelial-like Species: dog, cocker spaniel female; Tissue: kidney; Validated by isoenzymes: confirmed as dog with NP, G6D, MD, MPI, AST, PEPB Depositor: obtained from Flow Laboratories, Irvine (GB) Properties: expressing xenob |
epithelial-like | MDCK | Wanga, E. | Wanga, E., Browna, P.S., Aroetic, B., Chapinb, S.J., Mostovb, K.E., and Dunna, K.W., a Department of Medicine, Indiana University School of Medicine, Indianapolis, IN, USA b Department of Anatomy and Department of Biochemistry and Biophysics, University o | Indiana University | |||||||||
| Apical Localization Of A Functional Trpc3/Trpc6-Ca2-Signaling Complex In Polarized Epithelial Cells | 561 | Receptor-coupled
[Ca2 ]i increase is initiated in the apical region of epithelial cells and has been associated with apically localized Ca2 -signaling proteins. However, localization of Ca2 channels that are regulated by such Ca2-signaling events ha |
apically localized Ca2-signaling proteins, TRPC channels, plasma membrane receptors | Submandibular
glands were removed and placed in ice-cold external solution with 0.02% soybean trypsin inhibitor and 0.1% bovine serum albumin. Each gland was cleaned and finely minced. The minced tissue was transferred to 8 ml of external solution cont |
DOG, COCKER SPANIEL FEMALE | KIDNEY | IZSBS BS CL 64 Morphology: epithelial-like Species: dog, cocker spaniel female; Tissue: kidney; Validated by isoenzymes: confirmed as dog with NP, G6D, MD, MPI, AST, PEPB Depositor: obtained from Flow Laboratories, Irvine (GB) Properties: expressing xenob |
epithelial-like | MDCK | Bidhan C. | Bidhan C.
Bandyopadhyay, William D. Swaim, Xibao Liu, Robert S. Redman, Randen L. Patterson, and Indu S. Ambudkar |
National Institutes of Health | ||||||||||||||
| Aryl Phosphate Derivatives Of Bromo-Methoxy-Azidothymidine Are Dual-Function Spermicides With Potent Anti-Human Immunodeficiency Virus | 409 | Detergent-based
vaginal microbicides, in addition to their high contraceptive failure rates, cause mucosal erosion and local inflammation that might increase the risk of heterosexual human immunodeficiency virus (HIV) transmission. In a systematic effo |
vaginal
microbicides, mucosal erosion, inflammation, human immunodeficiency virus (HIV) transmission, aryl phosphate derivatives |
Frozen hamster
eggs (Charles River Laboratories, Wilmington, MA) were thawed and transferred to 35-mm glass-bottom microwells (Mat Tek Corporation, Ashland, MA) in BWW medium. To remove zona, the washed eggs were treated with 0.05% trypsin (Irvine Sci |
Biology of Reproduction | 59 | 1998 | September | 3 | BOVINE | capillary | ENDOTHELIAL | HAMSTER EGGS | D'Cruz, Osmond J. | Osmond J. D’Cruz, T.K. Venkatachalam, Zhaohai Zhu, Mei-Jue Shih, and Fatih M. Uckun | Wayne Hughes Institute | ||||||||||
| B Cells
Productively Engage Soluble Antigen-Pulsed Dendritic Cells: Visualization of Live-Cell Dynamics of B Cell-Dendritic Cell Interactions |
658 | Interactions between B lymphocytes and Ag-bearing dendritic cells (DC) likely occur at inflammatory sites and within lymphoid organs. To better understand these interactions we imaged B cells (TgB) from hen egg lysozyme (HEL) transgenic mice and DC pulsed | Three-dimensional
collagen matrix culture and cell collection Purified B cells and DC were labeled with Vybrant green CFDA SE (carboxy-fluorescein diacetate, succinimidyl ester) or Vybrant red DiI (Molecular Probes), respectively, following the manufactu |
mouse | lymph nodes | Dendritic Cell | Huang, Ning-Na | Ning-Na Huang, Sang-Bae Han, Il-Young Hwang, and John H. Kehrl | National Institute of Allergy and Infectious Diseases | |||||||||||||||||
| Biofilm Formation By The Fungal Pathogen Candida Albicans: Development, Architecture, And Drug Resistance | 433 | Biofilms are a
protected niche for microorganisms, where they are safe from antibiotic
treatment and can create a source of persistent infection. Using two clinically relevant Candida albicans biofilm models formed on bioprosthetic materials, we demonst |
Biofilms, antifungal | After incubation
with the dyes, polymethylmethacrylate strips or silicone elastomer disks were flipped and placed on a 35-mm-diameter glass-bottom petri dish (MatTek Corp., Ashland, Mass.). Stained biofilms were observed with a Zeiss LSM510 confocal sc |
Journal of Bacteriology | 183 | 2001 | September | 18 | HUMAN | GDH-2436 | Chandra, Jyotsna | JYOTSNA CHANDRA,
DUNCAN M. KUHN, PRANAB K. MUKHERJEE, LOIS L. HOYER, THOMAS MCCORMICK, AND MAHMOUD A. GHANNOUM |
University Hospitals of Cleveland | ||||||||||||
| Catalytic Activity Is Required For Calcium/Calmodulin-Dependent Protein Kinase Iv To Enter The Nucleus | 521 | Calcium/calmodulin-dependent
protein kinase IV (CaMKIV) is a nuclear protein kinase that responds to acute rises in intracellular calcium by phosphorylating and activating proteins involved in transcription. Consistent with these roles, CaMKIV is foun |
Catalytic Activity, Protein Kinase IV, calcium, nucleus, activation loop phosphorylation | HEK293A cells
were plated at a density of 0.1
106/35-mm glass-bottomed microwell dish (Mat Tek). The DNA:Opti-MEM ratio was 0.5 g of the indicated constructs (see figure legends) into 50 l of Opti-MEM (Invitrogen), and the LipofectAMINE: Opti-MEM r |
Journal of Biological Chemistry | 279 | 2004 | March | 12 | HUMAN | KIDNEY, EMBRYONIC | DSMZ ACC 305 Morphology: adherent fibroblastoid cells growing as monolayer human embryonal kidney established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Comm |
epithelial | HEK-293 | Lemrow, Shannon M. | Shannon M.
Lemrow, Kristin A. Anderson, James D. Joseph, Thomas J. Ribar, Pamela K. Noeldner, and Anthony R. Means |
Duke University | |||||||||
| Cellular Stresses Induce The Nuclear Accumulation Of Importin And Cause A Conventional Nuclear Import Block | 528 | We report here
that importin accumulates reversibly in the nucleus in response to cellular stresses including UV irradiation, oxidative stress, and heat shock. The nuclear accumulation of importin appears to be triggered by a collapse in the Ra |
nuclear import
block, cellular stresses, importin , Ran gradient |
Importin (mouse Rch1) and were constructed with pEGFP-C1
plasmid (CLONTECH Laboratories, Inc.). HeLa cells were plated onto a glass bottom dish (MatTek Corporation) cultured for 1–2 d before use. After the constructs were transfected using Effecten |
Journal of Cell Biology | 165 | 2004 | June June |
5 | HUMAN | cervix | IZSBS BS TCL20 Morphology: epithelial-like Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology |
epithelial-like | HeLa | Miyamoto, Yoichi | Yoichi
Miyamoto, Takuya Saiwaki, Junichi Yamashita, Yoshinari Yasuda, Ippei Kotera, Satoshi Shibata, Masaki Shigeta, Yasushi Hiraoka, Tokuko Haraguchi, and Yoshihiro Yoneda |
Osaka University | |||||||||
| Cellular Uptake Of N-Methylpyrrole/N-Methylimidazole Polyamide-Dye Conjugates | 335 | The cellular uptake and localization properties of DNA binding N-methylpyrrole/N-methylimidazole polyamide–dye conjugates in a variety of living cells have been examined by confocal laser scanning microscopy. With the exception of certain T-cell lines, po | Bioorganic and Medicinal Chemistry | 10 | 2002 | October | 10 | SKBR-3 | Belitsky, JM. | Belitsky, JM., Leslie, SJ., Arora, PS., Beerman, TA., Dervan, PB. Division of Chemistry and Chemical Engineering and Beckman Institute, California Institute of Technology, Pasadena, CA, USA; and Department of Pharmacology and Therapeutics, Roswel | California Institute of Technology | |||||||||||||||
| Ceramide Inhibition Of Nf-Kb Activation Involves Reverse Translocation Of Classical Protein Kinase C (Pkc) Isoenzymes: Requirement For Kinase Activityand Carboxyl-Terminal Phosphorylation Of Pkc For The Ceramide Response | 491 | Protein kinase
C (PKC) is known to activate NF-kB whereas the lipid mediator ceramide was recently shown to inhibit activation of this transcription factor (1, 2). In this study, the mechanisms by which ceramide interferes with this pathway were exami |
diacylglycerol, sphingolipid, glycerolipid, C6-ceramide | Jurkat T cells
and MCF7 adenocarcinoma cells were obtained from ATCC (Rockville, MD). TNF-a was purchased from PeproTech (Rocky Hill, NJ); C6-ceramide was purchased from Matreya (Pleasant Gap, PA); [g-32]ATP was purchased from NEN Life Science Product |
FASEB Journal | 15 | 2001 | November | 13 | HUMAN | BREAST | IZSBS
BS TCL 38 Morphology: epithelial-like Species: human, Caucasian female 69 years old; Tissue: breast; Tumor: adenocarcinoma; Validated by isoenzymes: confirmed as human with NP, G6PD, MD, AST Viruses: contains type C, type B Depositor: obtained from |
epithelial-like | MCF-7 | Signorelli, Paola | PAOLA SIGNORELLI, CHIARA LUBERTO, AND YUSUF A. HANNUN | Medical University of South Carolina | |||||||||
| Confocal Restricted-Height Imaging Of Suspension Cells (Crisc) In A Pdms Microdevice During Apoptosis | 451 | We have
monitored and imaged cell death induced in human leukemic U937 cells over
time using three-color confocal imaging. Three different apoptotic inducers, anti-Fas, TNF-a and Etoposide were used. Individual cascaded events such as loss of mitochondr |
apoptosis, CRISC method, caspases, apoptosome | Silicon
molds used for fabrication of the PDMS chips were realized using DRIE using the Bosch process. Molds with two different etch depths, 15 and 25 mm (Veeco Dektak 8 surface profiler), were fabricated. In Fig. 2 the three-dimensional structure of |
HUMAN | PLEURAL EFFUSION | Species: human, Caucasian male 37 years old; Tissue: pleural effusion; Tumor: lymphoma, histiocytic | histomonocytoid | U937 | Munoz-Pinedo, Cristina | Cristina Munoz-Pinedo, Douglas R. Green and Albert van den Berg | La Jolla Institute for Allergy and Immunology | ||||||||||||||
| Cytochrome C Is Released In A Single Step During Apoptosis | 517 | Release of
cytochrome c from mitochondria is a central event in apoptotic signaling. In this study, we utilized a cytochrome c fusion that binds fluorescent biarsenical ligands (cytochrome c-4CYS (cyt. c-4CYS)) as well as cytochrome c-green fluorescen |
apoptosis;
cytochrome c; mitochondria; mitochondrial membrane potential; video microscopy |
For time-lapse
analysis, adherent cells were grown in 35mm glassbottomed microwell dishes (MatTek), and treated in 3ml of phenol-red free DMEM (Gibco) supplemented with 10% FCS, 20mM HEPES (pH 7.3), 2mM L-glutamine, 200 mg/ml penicillin, 100 mg/ml stre |
Cell Death and Differentation | 12 | 2005 | May | 5 | NCI-H1299 | Goldstein, Jc | JC Goldstein, C Mun˜ oz-Pinedo, J-E Ricci, SR Adams, A Kelekar, M Schuler, RY Tsien and DR Green | La Jolla Institute for Allergy and Immunology | |||||||||||||
| Dendritic cell maturation triggers retrograde MHC class II transport from lysosomes to the plasma membrane | 615 | Central to the initiation of immune responses is recognition of peptide antigen by T lymphocytes. The cell biology of dendritic cells makes them ideally suited for the essential process of antigen presentation1. Their life cycle includes several stages ch | peptide antigen, T lymphocytes, antigen presentation, major histocompatibility complex class II molecules (MHC II), endocytosis, green fluorescent protein (GFP) | To label lysosomes, day 4–5 immature dendritic cells were…plated for 30min on poly-L-lysine pre-coated number 1.5 coverslips attached to 35-mm dishes (MatTek). | Nature | 2002 | July | P35GC-1.5 | Dendritic | Chow, Amy | Amy Chow, Derek Toomre, Wendy Garrett & Ira Mellman | Yale University | ||||||||||||||
| Development And Characterization Of An In Vivo Central Venous Catheter Candida Albicans Biofilm Model | 481 | Biofilms
represent a niche for microorganisms where they are protected from both the
host immune system and antimicrobial therapies. Biofilm growth serves as an increasing source of clinical infections. Candida infections are difficult to manage due to |
Candida
albicans, rat central venous catheter model, biofilm matrix |
Stained catheter
segments were transferred to a glass-bottom petri dish (coverslip 1.5, 35-mm disk P325G 1.5-14C; MatTek, Ashland, Mass.) in an on-end orientation. Biofilms were observed with a Nipkow disk-based confocal microscope (Axiovert 200; Zeiss |
Infection and Immunity | 72 | 2004 | October | 10 | P35G-1.5-14-C | YEAST | CATHETER SEGMENTS | Andes | D. Andes, J. Nett, P. Oschel, R. Albrecht, K. Marchillo, and A. Pitula | University of Wisconsin at Madison | |||||||||||
| Diacylglycerol And Protein Kinase D Localization During T Lymphocyte Activation | 419 | The serine
kinase protein kinase D (PKD) has a cysteine- rich domain (CRD) that binds diacylglycerol (DAG) with high affinity. PKD is cytosolic in unstimulated T cells, but it rapidly polarizes to the immunological synapse in response to antigen/antig |
kinase D (PKD), diacylglycerol (DAG) | Cells
were seeded onto glass coverslips in 24-well plates in conjugation medium (RPMI medium without phenol red/0.5 % FCS) and allowed to attach for 20 min at 37ºC. T cells were treated with either 200 nM phorbol-12,13-dibutyrate (PdBu, Calbiochem) or |
Immunity | 24 | 2006 | May | 5 | THYMUS | lymphocyte | T. | Spitaler, Martin | Martin Spitaler, Elisabeth Emslie, C. David Wood, and Doreen Cantrell | University of Dundee | |||||||||||
| Different Populations Of Rna Polymerase Ii In Living Mammalian Cells | 540 | RNA polymerase
II is responsible for transcription of most eukaryotic genes, but, despite
exhaustive analysis, little is known about how it transcribes natural templates in vivo. We studied polymerase dynamics in living Chinese hamster ovary cells using |
DRB, FLIP, heat shock, photobleaching, RNA polymerase II | Cells were grown
in glass-bottomed microwell dishes (Mat Tek, MA) for 40–48 h to 50% confluence. The standard FLIP experiment in Figure 1 was performed as described by Kimura et al. (2002) using a Radiance 2000 confocal microscope (488-nm-laser line; |
neuronal | MYOCYTES | Hieda, Miki | Miki Hieda, Henry Winstanley, Philip Maini, Francisco J. Iborra & Peter R. Cook | University of Oxford | |||||||||||||||||
| Differential Gel Electrophoresis And Transgenic Mitochondrial Calcium Reporters Demonstrate Spatiotemporal Filtering In Calcium Control Of Mitochondria | 396 | Mitochondria must adjust both their intracellular location and their metabolism in order to balance their output to the needs of the cell. Here we show by the proteomic technique of time series difference gel electrophoresis that a major result of neuroen | gel electrophoresis, neuropeptide signalling | Adult flies were
dissected in Schneider’s Drosophila medium (Invitrogen). For vital imaging of V-ATPase expression with the vhaSFD::GFP gene trap line (5), tubules were allowed to adhere lightly to a poly-Llysine treated coverslip glued to a window in the |
Journal of Biological Chemistry | 281 | 2006 | July | 27 | Mitochondria | Terhzaz, Selim | Selim Terhzaz,
Tony D. Southall, Kathryn S. Lilley, Laura Kean, Adrian K. Allan, Shireen A. Davies and Julian A. T. Dow |
University of Glasgow | |||||||||||||
| Differential Kinetic and Spatial Patterns of -Arrestin and G Protein-mediated ERK Activation by the Angiotensin II Receptor | 631 | The seven-membrane-spanning angiotensin II type 1A receptor activates the mitogen-activated protein kinases extracellular signal-regulated kinases 1 and 2 (ERK1/2) by distinct pathways dependent on either G protein (likely Gq/G11) or -arrestin2. Here we | angiotensin II type 1A receptor, G protein, B-arrestin2, nuclear translocation, cytoplasmic endosomal vesicles | One day after transfection, cells were divided into collagen-coated 35-mm glass bottom dishes (MatTek, Ashland, MA) for confocal microscopy. | Journal of Biological Chemistry | 279 | 2004 | August | 34 | P35GCOL | HEK-293 | Ahn, Seungkirl | Seungkirl Ahn, Sudha K. Shenoy, Huijun Wei, and Robert J. Lefkowitz | HHMI | ||||||||||||
| Direct Evidence Of Active And Rapid Nuclear Degradation Triggered By Vacuole Rupture During Programmed Cell Death In Zinnia | 255 | Differentiation into a tracheary element (TE) is a typical example of programmed cell death (PCD) in the developmental processes of vascular plants. In the PCD process the TE degrades its cellular contents and becomes a hollow corpse that serves as a wate | After stained cells were transferred onto poly-d-Lyscoated dishes (Glass Bottom Microwell Dishes, MatTek Corporation, Ashland, MA), they were placed on the inverted platform of a confocal laser scanning microscope (Meridian Instruments Far East, Tokyo). | Plant Physiol | 125 | 2001 | Mesophyll | Obara, K. | Obara, K., Kuriyama, H., and Fukuda, H., Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan. Plant Physiology, (5), 65-66, (00). | University of Tokyo | ||||||||||||||||
| Disruption of nucleocytoplasmic trafficking of cyclin D1 and topoisomerase II by sanguinarine | 594 | The quaternary isoquinoline alkaloid sanguinarine is receiving increasing attention as a potential chemotherapeutic agent in the treatment of cancer. Previous studies have shown that this DNA-binding phytochemical can arrest a number of different types of | breast cancer, sanguinarine, DNA synthesis | Cells were seeded into 35 mm glass-bottom Petri dishes (MatTek Inc., Amherst, MA) at a density of 2.5 × 104/ml, and allowed to recover for 2 days prior to use. | BioMed Central Cell Biology | 7 | 2006 | March | 13 | Breast | MCF-7 | Holy, Jon | Jon Holy, Genelle Lamont and Edward Perkins | University of Minnesota | ||||||||||||
| Distinct Sites In E-Cadherin Regulate Different Steps In Drosophila Tracheal Tube Fusion | 558 | We have
investigated how E-cadherin controls the elaboration of adherens junction associated cytoskeletal structures crucial for assembling tubular networks. During Drosophila development, tracheal branches are joined at branch tips through lumens tha |
E-cadherin, Drosophila, Cytoskeleton | Primary
antibodies were: rabbit anti-EB1 (Rogers et al., 2002) (1:500), rabbit anti-CLIP190 (Lantz and Miller, 1998) (1:1000), rabbit anti-b-galactosidase (Sigma), rat anti-tubulin (Serotec), rat mAb anticadherin (1:5) (Oda et al., 1994), mouse mAb ant |
Development | 130 | 2003 | December | 23 | Drosophila melanogaster | EMBRYO | DSMZ ACC 130 Morphology: small adherent cells growing in monolayers; a small number of cells is also in suspension insect - fruitfly (Drosophila melanogaster) established from the late embryo of a Drosophila melanogaster (fruitfly) in 1972; originally the |
small adherent cells growing
in monolayers; a small number of cells is also in suspension insect |
SCHNEIDER S2 | Lee, Mihye | Mihye Lee, Seungbok Lee, Alireza Dehghani Zadeh, and Peter A. Kolodziej | Vanderbilt University | |||||||||
| Dopaminergic Stimulation of Local Protein Synthesis Enhances Surface Expression f GluR1 and Synaptic Transmission in Hippocampal Neurons | 586 | The use-dependent modification of synapses is strongly influenced by dopamine, a transmitter that partici-pates in both the physiology and pathophysiology of animal behavior. In the hippocampus, dopaminergic signaling is thought to play a key role in prot | dopamine, synaptic plasticity, dendrites, AMPA receptors, green fluorescent protein (GFP) | Briefly, hippocampi from postnatal day 2 Sprague-Dawley rat pups were enzymatically and mechanically dissociated and plated into poly-lysine-coated glass-bottom petri dishes (Mattek). | poly-d-lysine coated | rat | neuronal | HIPPOCAMPAL | Smith, W. Bryan | W. Bryan Smith, Shelley R. Starck, Richard W. Roberts, and Erin M. Schuman | HHMI | |||||||||||||||
| Dynamic Analysis Of Stat6 Signalling In Living Cells | 224 | Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP–STAT6) fusion protein appeared to be more active | STAT6; NF-kappaB; Confocal microscopy; Green fluorescent protein | For confocal microscopy, cells were plated onto 35-mm Mattek dishes (Mattek, USA) at 1.6×105 cells in 2 ml of medium. HeLa cells (ECACC No. 93021013) were grown in MEM with Earle’s salts, plus 10% FCS, and 1% NEAA at 37°C, 5% CO2…. | HUMAN | KIDNEY, EMBRYONIC | DSMZ ACC 305 Morphology: adherent fibroblastoid cells growing as monolayer human embryonal kidney established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Comm |
epithelial | HEK-293 | Nelsona, G. | Nelsona, ,G.,
Wildea, , G., Spillera, D., Sullivanb, E., Unittb , J., and White, M.,
a School of Biological Sciences, University of Liverpool, Liverpool, United
Kingdom b Lead Generation, Molecular Biology, AstraZeneca R&D Leicestershire, United King |
University of Liverpool | ||||||||||||||
| Dynamin 2 Is Required For Phagocytosis In Macrophages | 417 | Cells
internalize soluble ligands through endocytosis and large particles through
actin-based phagocytosis. The dynamin family of GTPases mediates the scission of endocytic vesicles from the plasma membrane. We report here that dynamin 2, a ubiquitously |
phagocytosis, macrophages, dynamin, inflammation, phosphoinositide 3-kinase | RAW-TT10 cells
were transfected with dyn K44A -pTIGZ and plated on 35-mm glass-bottomed microwell dishes (MatTek Corp.). 18 h later, the dish was mounted on a Zeiss Axiophot microscope equipped with a cooled CCD camera (Princeton Instruments) and Me |
Journal of Experimental Medicine | 190 | 1999 | December | 12 | RAW-TT10 | Gold, Elizabeth S. | Elizabeth S.
Gold, David M. Underhill, Naomi S. Morrissette, Jian Guo, Mark A. McNiven, and Alan Aderem |
University of Washington | |||||||||||||
| Effects Of Human Antithymocyte Globulin On Acetylcholine Synthesis, Its Release And Choline Acetyltransferase Transcription In A Human Leukemic T-Cell Line | 229 | In human skin both resident and transiently residing cells are part of the extra- or non-neuronal cholinergic system, creating a highly complex and interconnected cosmos in which acetylcholine (ACh) and choline are the natural ligands of nicotinic and mus | Acetylcholine; Choline acetyltransferase; T-lymphocyte | Cover
glass-bottomed culture dishes coated with poly-D-lysine (#P35GC-0-14-C) were
from MatTek (Ashland, MA). AND Once loaded with fluo-3, the cells were diluted 1:9 with RPMI 1640, incubated for an additional 30 min, washed with Tyrode-HEPES buffer (20 |
Journal of Neuroimmunology | 128 | 2002 | July | 1 | P35GC-0-14-C | HUMAN | Tumor: leukemia | Morphology:
lymphoblast-like Species: human; Tumor: leukemia, acute lymphoblastic; Derived from: CCRF-CEM Properties: resistant to 8-azaguanine; pharmacodynamics; cloning |
lymphoblast-like | CEM | Fujii, T. | Fujii, T., Ushiyama, N., Hosonuma, K., Suenaga A., and Kawashima K., Department of Pharmacology, Kyoritsu College of Pharmacy, Tokyo, Japan. Journal of Neuroimmunology, 8, (-), -8, (00) | Kyoritsu College of Pharmacy | ||||||||
| Exocytotic Release of ATP from Cultured Astrocytes | 674 | Astrocytes
appear to communicate with each other as well as with neurons via ATP. However, the mechanisms of ATP release are controversial. To explore whether stimuli that increase [Ca2+]i also trigger vesicular ATP release from astrocytes, we labeled ATP |
The cells were
plated at a density of 1.5·105 cells/cm2 on MatTek dishes pre-treated with poly-L-lysine. |
Journal of Biological Chemistry | 282 | 2007 | September | 39 | MatTek dishes pretreated with poly-l-lysine | Hippocampi | Wistar Rat Embryo | E18 | Pangrsic, Tina | Tina Pangrsic, Maja Potokar, Matjaz Stenovec, Marko Kreft, Elsa Fabbretti, Andrea Nistri, Evgeny Pryazhnikov, Leonard Khiroug, Rashid Giniatullin, and Robert Zorec | Univ of Ljubljana, Celica Biomedical Center, University of Helsinki, International School for Advanced Studies | |||||||||||
| Experimental Study On Bone Remodeling Rate Equation: Effects Of Mechanical Stimulus On Osteoblastic Activities In Vitro | 556 | An experimental
study in vitro at the cellular level is fundamental to clarify the mechanism for adaptive bone remodeling. Many efforts have been made to study the cellular responses to mechanical stimuli in vitro, however, the mechanism for the mechano |
BONE REMODELING, osteoblastic activities, cultured osteoblasts, osteoblastic proliferation, | Osteoblasts were
plated on a glass-bottomed dish (Mat-Tech) and loaded by exposure to 10µM Fluo3/AM (Wako Chemicals), a fluorescence indicator of Ca2+, at 37 Co for 2 hours. After being rinsed with phosphate-buffered saline (PBS), the dish was mounted |
Comput Biomech | MC3T3-EC | Hoshiai, Sodai | Sodai HOSHIAI, Taiji ADACHI, and Yoshihiro TOMITA | Kobe University | |||||||||||||||||
| Fluorescent dyes alter intracellular targeting and function of cell-penetrating tetrapeptides | 639 | Fluorescent labels are commonly used to investigate the mechanisms of cellular uptake and intracellular distribution of cell-penetrating peptides. However, labels such as fluorescein and rhodamine are relatively large and very lipophilic and may significa | membrane transduction, mitochondria, permeability transition, cytoplasm, confocal microscopy | Caco-2 cells were plated on 35 mm glass bottom dishes (MatTek, Ashland, MA) for 2 days. | P35GC-0-10-C | epithelial | Caco-2 | Szeto, Hazel H. | Hazel H. Szeto, Peter W. Schiller, Kesheng Zhao, and Guoxiong Luo | Weill Medical College | ||||||||||||||||
| Fluorogenic Substrate [Ala-Pro] 2-Cresyl Violet But Not Ala-Pro-Rhodamine 110 Is Cleaved Specifically By Dppiv Activity: A Study In Living Jurkat Cells And Cd26/Dppiv-Transfected Jurkat Cells | 454 | Fluorogenic
substrates [Ala-Pro] 2 -cresyl violet and Ala-Pro-rhodamine 110 have been tested for microscopic detection of protease activity of dipeptidyl peptidase IV (DPPIV) in living cells. DPPIV activity is one of the many functions of the multifun |
living cell
cytochemistry, protease, metabolic mapping, biocomplexity, CD26/DPPIV, Jurkat cells, confocal scanning laser microscopy, fluorescence microscopy |
Confocal
analysis was performed to localize DPPIV-like activity in living Jurkat and transfected Jurkat cells on a Leica SP 2 AOBS confocal microscope (Leica Microsystems; Mannheim, Germany). In case of the use of [Ala-Pro] 2 -cresyl violet, fluor |
Journal of Histochemistry and Cytochemistry | 51 | 2003 | July | 7 | Jurkat | Boonacker, Emil | Emil Boonacker,
Sjoerd Elferink, Abdennasser Bardai, Bernard Fleischer, and Cornelis J.F. Van Noorden |
University of Amsterdam | |||||||||||||
| Four Naturally Occurring Mutations In The Human Gnrh Receptor Affect Ligand Binding And Receptor Function | 253 | In the present study, we performed functional analyses of four mutations in the human GnRH receptor (GnRHR) gene, identified in patients with idiopathic hypogonadotropic hypogonadism. These mutations result in amino acid substitutions in the extracellular | GnRH; GnRH receptor; Gonadotrope; Signal transduction | COS-7 cells were plated on glass bottom 35 mm tissue culture dishes (MatTek Corp., Ashland, MA), and transiently transfected using GenePorter transfection reagent (Gene Therapy System, San Diego, CA) with 2 µg of wild type or mutant hGnRHR cDNA constructs | Molecular and Cellular Endocrinology | 205 | 2003 | July | 1-2 | MONKEY, AFRICAN GREEN | KIDNEY | IZSBS BS CL103 Morphology: fibroblast-like Species: monkey, African green; Tissue: kidney; Transformed by: SV40; Original line: CV-1 Properties: virus replication |
fibroblast-like | COS-7 | Bédécarratsa,G.Y | Bédécarratsa,G.Y., Linhera,K.D., Janovickb, JA., Beranovac, M., Kadaa, F., Seminarac, S.B., Connb, P. M., and Kaisera, U.B., a Division of Endocrinology, Diabetes and Hypertension, Department of Medicine, Brigham and Women's Hospital and Harvard Medical | Brigham and Women's Hospital | |||||||||
| Four-Dimensional Imaging And Computer-Assisted Track Analysis Of Nuclear Migration In Root Hairs Of Arabidopsis Thaliana | 258 | Nuclear migration is a fundamental mechanism necessary for the proper growth and development of many eukaryotic organisms. In this study root hairs of Arabidopsis thaliana were used as a research model to gain insight into the dynamics of nuclear migratio | four-dimensional imaging, live cell imaging, nuclear migration, root hairs, track analysis | “The seedlings were transferred to single chambered cover glasses (Labtek II form Nunc plastic ware or Micro well dishes form Mattek. U.S.A.) for microscopic observation.” | Journal of Microscopy | 211 | 2003 | 2 | VASCULAR | endothelial s | SEEDLINGS | Van Bruaene, N. | Van Bruaene, N., Joss, G., O. Thas, O., and Van Oostveldt, P., Laboratory for Biochemistry and Molecular Cytology, Ghent University, Gent, Belgium, Department of Biological Science, Macquarie University, Sydney Australia, Department of Applied M | Ghent University | ||||||||||||
| Ganglioside/Calmodulin Kinase Ii Signal Inducing Cdc42-Mediated Neuronal Actin Reorganization | 249 | Cell surface glycoconjugates are thought to mediate cell–cell recognition and play roles in neuronal development and functions. We demonstrated here that exposure of neuronal cells to nanomolar levels of gangliosides Neu5Ac8Neu5Ac3Galβ4GlcCer, Galβ3GalNAc | CaM-kinase II; filopodia; oligosaccharide; NG108-15 cell; hippocampal neuron; Purkinje cell | Cells cultured on poly-lysine coated glass-bottom dish (35 mm, MatTek Co., Ashland, MA, USA). | Neuroscience | 120 | 2003 | 1 | HIPPOCAMPAL | NEURONAL | Chena | Chena, b, N., Furuyac, S., Doib, H., Hashimotob, Y., Kudod, Y., and Higashia,a, b, H., a Mitsubishi Kagaku Institute of Life Sciences, Tokyo, Japan b Glyco-Chain Functions Laboratory, RIKEN Frontier Research System, Saitama, Japan c Neuronal Circuit Mecha | Institute of Life Sciences | |||||||||||||
| GBF1, a cis-Golgi and VTCs-localized ARF-GEF, is implicated in ER-to-Golgi protein traffic | 614 | The formation and maturation of membrane carriers that transport cargo from the ER to the Golgi complex involves the sequential action of the coat protein complexes COPII and COPI. Recruitment of COPI to nascent carriers requires activation of ADP-ribosyl | GBF1, ERGIC, VTCs, ARF-GEF, Brefeldin A, Protein traffic | For Fig. 7, NRK-GFP-GBF1 cells were grown on Plastek Cultureware glass bottom microwell dishes (MatTek Corp) and imaged on the temperature-controlled stage of a Zeiss Axiovert 200M microscope equipped with an UltraVIEW ERS 3E spinning disk. | NRK | Zhao, Xinhua | Xinhua Zhao, Alejandro Claude, Justin Chun, David J. Shields, John F. Presley and Paul Melançon, | University of Alberta | ||||||||||||||||||
| Global Survey Of Organ And Organelle Protein Expression In Mouse: Combined Proteomic And Transcriptomic Profiling | 347 | A global protein survey is needed to gain systems-level insights into mammalian cell signaling and information flow. Human Jurkat T leukemic cells are one of the most important model systems for T cell signaling study, but no comprehensive proteomics surv | mouse, subcellular factionation, transcriptional profiling, proteomic profiles, putative transmembrane helices (TMH), | For these
experiments, we generated sequence-verified clones for ten representative
gene products as N-terminal fusions to a green fluorescent marker protein
(GFP) and examined their corresponding localization patterns in transient transfections of HEK |
Molecular and Cellular Proteomics | 6 | 2007 | August | 8 | P35G-0-10-C | HUMAN | KIDNEY, EMBRYONIC | DSMZ ACC 305 Morphology: adherent fibroblastoid cells growing as monolayer human embryonal kidney established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Comm |
epithelial | HEK-293 | Kislinger, Thomas | Thomas
Kislinger, Brian Cox, Anitha Kannan, Clement Chung, Pingzhao Hu, Alexandr
Ignatchenko, Michelle S. Scott, Anthony O. Gramolini, Quaid Morris, Michael T. Hallett, Janet Rossant, Timothy R. Hughes, Brendan Frey, and Andrew Emili |
University of Toronto | ||||||||
| Group I Metabotropic Glutamate Receptor Inhibition Selectively Blocks A Prolonged Ca2+ Elevation Associated With Age-Dependent Excitotoxicity | 227 | The aim of the present paper was to examine, in a comparative way, the occurrence and the mechanisms of the interactions between adenosine A2A receptors (A2ARs) and metabotropic glutamate 5 receptors (mGlu5Rs) in the hippocampus and the striatum. In rat h | hippocampus; cell culture; aging; NMDA; AIDA; neurotoxicity | For confocal imaging studies, the cell suspension was diluted with MEM to a final concentration of ~5×105 cells/ml and 1 ml added to poly-L-lysine-coated (1 mg/ml) glass-bottom culture dishes (35 mm; MatTek, Ashland, MA, USA) containing 1 ml MEM with 10% | Purinergic Signalling | 2 | 2006 | November | 4 | RAT | FETAL | neuronal | Cerebellar granule s | Attuccib, S. | Attuccib,, S., Clodfeltera, ,G. V., Thibaulta, O., Statona, J., Moronib, F., Landfielda , P. W. , and Porter a, N. M., a
Department of Molecular and Biomedical Pharmacology, University of Kentucky
College of Medicine, Lexington, KY, USA. b Departi |
University of Kentucky | ||||||||||
| Heterotrimeric G Proteins Precouple With G Protein-Coupled Receptors In Living Cells | 480 | Using
fluorescence resonance energy transfer (FRET) microscopy, we investigate how heterotrimeric G proteins interact with G protein-coupled receptors (GPCRs). In the absence of receptor activation, the 2A adrenergic and muscarinic M4 receptors are p |
heterotrimeric
G proteins, G protein-coupled receptors (GPCRs), G protein-gated K channel |
Cells for
imaging were subcultured onto 25-mm glass coverslips and then placed into a watertight cell imaging chamber at room temperature, or were subcultured onto 35-mm culture dishes with integral no. 0 glass coverslip bottoms (Mattek). |
PNAS | 102 | 2005 | December | 51 | HUMAN | KIDNEY, EMBRYONIC | DSMZ ACC 305 Morphology: adherent fibroblastoid cells growing as monolayer human embryonal kidney established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Comm |
epithelial | HEK-293 | Nobles, Muriel | Muriel Nobles, Amy Benians, and Andrew Tinker | British Heart Foundation Laboratories | |||||||||
| Hfis1, A Novel Component Of The Mammalian Mitochondrial Fission Machinery | 525 | The balance
between the fission and fusion mechanisms regulate the morphology of mitochondria. In this study we have identified a mammalian protein that we call hFis1, which is the orthologue of the yeast Fis1p known to participate in yeast mitochondr |
Mitochondrial Fission, hFis1, dominant negative mutant of Drp1, apoptosis, Mitochondria |
COS-7 or HeLa
cells were plated at 2.5 105 cells/3-cm glass bottomed dish (Mattek) 6 h prior to transfection. Cells were then transfected using FuGENE (Roche Applied Science) with a 1:1 ratio of target DNA to YFP-mito (Clontech) and a total of 1 g |
Journal of Biological Chemistry | 278 | 2003 | September | 38 | MONKEY, AFRICAN GREEN | KIDNEY | IZSBS BS CL103 Morphology: fibroblast-like Species: monkey, African green; Tissue: kidney; Transformed by: SV40; Original line: CV-1 Properties: virus replication |
fibroblast-like | COS-7 | James, Dominic I. | Dominic I. James, Philippe A. Parone, Yves Mattenberger, and Jean-Claude Martinou | University of Geneva | |||||||||
| Histone acetylation increases chromatin accessibility | 636 | In eukaryotes, the interaction of DNA with proteins and supramolecular complexes involved in gene expression is controlled by the dynamic organization of chromatin inasmuch as it defines the DNA accessibility. Here, the nuclear distribution of microinject | Heterochromatin, Image correlation spectroscopy, Trichostatin A, Microinjection | HeLa cells (ATCC: CCL-2) were cultured on glass coverslips for fixed cell analysis and on MatTek glass bottom dishes (MatTek, Ashland, MA) for in vivo imaging in DMEM for 1 day. | Journal of Cell Science | 118 | 2005 | December | 24 | HeLa | Gorisch, Sabine M. | Sabine M. Görisch, Malte Wachsmuth, Katalin Fejes Tóth, Peter Lichter and Karsten Rippe | Deutsches Krebsforschungszentrum | |||||||||||||
| HIV-1 Nef mediates post-translational down-regulation and redistribution of the mannose receptor | 601 | Human immunodeficiency virus (HIV) has derived a variety of means to evade the host immune response. HIV-derived proteins, including Tat, Nef, and Env, have all been reported to decrease expression of host molecules such as CD4 and major histocompatibilit | macrophages, trafficking, HIV-derived proteins | Transfection of cells for confocal experiments was performed in 35 mm glass-bottom dishes (MatTek, Ashland, MA). Briefly, 5 105 cells were seeded into each dish and incubated overnight. | HeLa | Vigerust, David J. | David J. Vigerust, Brian S. Egan, and Virginia L. Shepherd | Vanderbilt University | ||||||||||||||||||
| Human Lung Cancer Cells Express Functionally Active Toll-Like Receptor 9 | 333 | Here we show that TLR9 is expressed in a selection of human lung cancer tissues and various tumor cell lines. The expression of functionally active TLR9 in human malignant tumors might affect treatment approaches using CpG-ODN and shows that malignant cel | A549-cells were seeded in 35-mm glass bottom dishes (MatTek Corp.) overnight. Cells were transfected with GFP-huTLR9 using Polyfect (Qiagen) according to the manufacturer's instructions or incubated in medium. | HUMAN | LUNG CARNINOMA | DSMZ
ACC 107 Morphology: epithelial cells, growing adherently as monolayer human lung carcinoma established from an explanted lung tumor which was removed from a 58-year-old Caucasian man in 1972; cells were described to induce tumors in athmyic mice and |
EPITHELIAL | adipocyte | Droemann, D. | Droemann, D., Albrecht, , D., Gerdes, J., Ulmer, AJ., Branscheid, D., Vollmer4, E., Dalhoff5, K., Zabel,5 , P., Goldmann4, T. Medical Clinic, Research Center Borstel, Borstel, Germany; Department of Immunology and Cell Biology, Research Center | Research Center Borstel | |||||||||||||||
| Hydrogen Peroxide-Induced Cell Death In Cultured Aplysia Sensory Neurons | 381 | Widespread neuronal cell death occurs during normal development and as a result of pathological conditions in the nervous system of many organisms. In this study, we investigated the cytotoxicity induced by H2O2 in Aplysia mechanosensory neurons, which se | Aplysia; Neuronal cell death; Hydrogen peroxide; Membrane potential; Input resistance | Approximately
50–150 neurons were plated on poly-L-lysine (Sigma Chemical Co., St. Louis,
MO) coated culture dishes (P50G-0-14-F) containing L15 media with filtered hemolymph (1:1). Pleural ganglia were also organotypically cultured on the culture dishes |
Brain Research | 941 | 2002 | June | 1-2 | P50G-0-14-F | HUMAN | AIRWAY | neuronal | HASM | Chae-Seok Lim | Chae-Seok Lim, Jong-Cheol Lee, Su Dong Kim, Deok-Jin Chang, Bong-Kiun Kaang | Seoul National University | |||||||||
| In vitro assays for evaluating the ultraviolet B-induced damage in cultured human retinal pigment epithelial cells | 710 | The present
study demonstrates broadband UV-B-induced damage of cultured human retinal
pigment epithelial cells as an effort to develop an in vitro model that can be used, along with in vivo research and other in vitro efforts, to evaluate the need for re |
UVB-induced
retinal damage; RPE; Alamar blue assay; Cellular viability; Confocal
microscopy; Mitochondrial damage; DNA damage; Phagocytotic activity |
In the present
study, 1 · 105 cells in 1.5 ml of culture medium were seeded into collagen coated glass bottom culture Petri dishes (MatTek, MA, USA). |
Collagen coated glass bottom dishes | human | retinal | epithelial | ARPE-19 | Youn, Hyun-Yi | Hyun-Yi Youn, Vladimir Bantseev, Niels C. Bols, Anthony P. Cullen, Jacob G. Sivak - Not in ACT | University of Waterloo | ||||||||||||||
| In Vivo Activation Of Pro-Form Bombyx Cysteine Protease (Bcp) In Silkmoth Eggs: Localization Of Yolk Proteins And Bcp, And Acidification Of Yolk Granules | 234 | The present study was designed to investigate the process of acidification of yolk granules during embryogenesis. In oocytes of mature Bombyx mori silkmoth, yolk proteins and a cysteine protease (pro-form BCP) were found in yolk granules. BCP was localize | Bombyx acid cysteine protease; yolk protein; lysosomal acidification; embryonic development | Oocytes and eggs were broken with fine tungsten needles in buffer A containing 0.01% acridine orange. They were placed in poly-Lysine-coated glass-bottom dishes (MatTek Corp., Ashland, MA) and incubated for 5–10 min at room temperature in the dark. | Journal of Insect Physiology | 49 | 2003 | February | 2 | OOCYTES | Yamahama, Y. | Yamahama, Y., a,
Uto, N.,a, Tamotsu S.,b, T. Miyatac, Yamamoto, Y.d, Watabe, S.e and
Takahashi, S. Y. d, a Department of Biology, Hamamatsu University, Hamamatsu,
Japan, b Department of Biological Science, Nara Women's University, Nara,
Japan, c, d, Dep |
Hamamatsu University School of Medicine | |||||||||||||
| In Vivo Near-Infrared Fluorescence Imaging Of Integrin V 3 In Brain Tumor Xenografts | 376 | Noninvasive
visualization of cell adhesion molecule v3 integrin expression in vivo has been well studied by using the radionuclide imaging modalities in various preclinical tumor models. A literature survey indicated no previous use of cyanine dyes as co |
cell adhesion
molecule v 3, subcutaneous U87MG glioblastoma xenograft, Integrins v3 and
v5, tumor growth and metastasis, tumor angiogenesis,
arginine-glycine-aspartic acid (RGD) peptides |
For laser
confocal microscopy, cells grown on 35-mm MatTek imaging dishes (Cat no. P35G-0- 14-C, Ashland, MA) were washed with PBS and then were incubated at 37°C in the presence of 100 nmol/L RGD-Cy5.5 for 1 hour. Afterward, cells were washed in ice- |
Cancer Research | 64 | 2004 | November | 21 | P35G-0-14-C | Tumor | Chen, Xiaoyuan | Xiaoyuan Chen, Peter S. Conti, and Rex A. Moats | University of Southern California (USC) | ||||||||||||
| Increased Reactive Oxygen Species Contribute To High Nacl-Induced Activationof The Osmoregulatory Transcription Factor Tonebp/Orebp | 529 | activation of
the transcription factor tonicity-responsive enhancer binding protein/osmotic response element binding protein (TonEBP/OREBP) remain incompletely understood. High NaCl has been reported to produce oxidative stress. Reactive oxygen specie |
superoxides; nitric oxide; ouabain; transactivation; nuclear translocation | Dihydroethidium
(DHE) enters cells freely. Superoxides oxidize DHE into ethidium or a structurally similar product, which intercalates into DNA, producing fluorescence (5). One to 1.2 106 cells were grown overnight on the optical glass bottom of a d |
American Journal of Physiology- Renal Physiology | 289 | 2005 | August | 2 | HUMAN | KIDNEY, EMBRYONIC | DSMZ ACC 305 Morphology: adherent fibroblastoid cells growing as monolayer human embryonal kidney established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Comm |
epithelial | HEK-293 | Zhou, Xiaoming | Xiaoming Zhou, Joan D. Ferraris, Qi Cai, Anupam Agarwal, and Maurice B. Burg | Uniformed Services University of the Health Sciences | |||||||||
| Interaction Of A Farnesylated Protein With Renal Type Iia Na/Pi Co-Transporter In Response To Parathyroid Hormone And Dietary Phosphate | 405 | Treatment with PTH (parathyroid hormone) or a high-P(i) diet causes internalization of the type IIa sodium-dependent phosphate (Na/P(i) IIa) co-transporter from the apical membrane and its degradation in the lysosome. A dibasic amino acid motif (KR) in th | endocytosis,
internalization, peroxisomal protein, peroxisomal farnesylated protein (PEX19) |
OK cells were
obtained from the American Type Culture Collection and maintained in an appropriate medium [20]. For transfection,OKcells were plated in a 35 mmglass-bottomed dish (MattekCorp.,Ashland, MA,U.S.A.) at a density of 2×105 cells. Cultures at sub |
MONKEY, AFRICAN GREEN | KIDNEY | IZSBS BS CL103 Morphology: fibroblast-like Species: monkey, African green; Tissue: kidney; Transformed by: SV40; Original line: CV-1 Properties: virus replication |
fibroblast-like | COS-7 | Ito, Mikiko | Mikiko ITO,
Sachi IIDAWA, Michiyo IZUKA, Sakiko HAITO, Hiroko SEGAWA, Masashi KUWAHATA,
Ichiro OHKIDO, Hiroshi OHNO and Ken-ichi MIYAMOTO |
Tokushima University School of Medicine | ||||||||||||||
| Interaction of Polycationic Polymers with Supported Lipid Bilayers and Cells: Nanoscale Hole Formation and Enhanced Membrane Permeability | 598 | Interactions of polycationic polymers with supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) lipid bilayers and live cell membranes (KB and Rat2) have been investigated using atomic force microscopy (AFM), cytosolic enzyme assays, confocal lase | polycationic polymers, lipid bilayers, lactate dehydrogenase, luciferase, membrane permeabilit | A concentration of 2 104 cells/mL of Rat2 cells was seeded on MatTek glass bottom Petri dishes (35 mm) and incubated at 37 °C under 5% CO2 for 24 h. | Biconjugate Chemistry | 17 | 2006 | May | 3 | rat | Rat2 | Hong, Seungpyo | Seungpyo Hong, Pascale R. Leroueil, Elizabeth K. Janus, Jennifer L. Peters, Mary-Margaret Kober, Mohammad T. Islam, Bradford G. Orr, James R. Baker, Jr., and Mark M. Banaszak Holl | University of Michigan | ||||||||||||
| Interaction With Gm130 During Herg Ion Channel Trafficking | 362 | Many mutations in the Human Ether-à-go-go-Related Gene (HERG) cause type 2 congenital long QT syndrome (LQT2) by disrupting trafficking of the HERG-encoded potassium channel. Beyond observations that some mutations trap channels in the endoplasmic reticul | Human Ether-a`
-go-go-Related Gene (HERG), GM130, long QT syndrome, terminal glycosylation, oocytes |
The C terminus
of HERG was used to probe for interacting proteins expressed from a human heart library using the yeast two-hybrid technique. We screened 2.2 106 independent clones from a human heart cDNA library consisting of recombinant plasmids fus |
P35GC-0-14-C | HUMAN | KIDNEY, EMBRYONIC | DSMZ ACC 305 Morphology: adherent fibroblastoid cells growing as monolayer human embryonal kidney established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Comm |
epithelial | HEK-293 | Roti, Elon C. | Elon C. Roti
Roti, Cena D. Myers, Rebecca A. Ayers, Dorothy E. Boatman, Samantha A. Delfosse, Edward K. L. Chan, Michael J. Ackerman, Craig T. January, and Gail A. Robertson |
University of Wisconsin at Madison | |||||||||||||
| Interception Of Small Particles By Flocculent Structures, Sessile Ciliates, And The Basic Layer Of A Wastewater Biofilm | 560 | We investigated
attachment processes of hydrophobic and hydrophilic particles (diameter 5 1
mm) to mature biofilms grown on clay marbles in a sequencing batch biofilm reactor. During a treatment cycle with filtered wastewater containing different fluore |
Wastewater Biofilm, particle retention, ciliate bioturbation, ciliates, adsorption, sequencing batch technology | Particle density
in the basic biofilm layer and in food vacuoles of sessile ciliates was estimated by automated analysis of CLSM images. Image acquisition was performed with a Zeiss (Jena, Germany) 510 confocal laser scanning microscope. An argon laser |
HYDROPHOBIC | Eisenmann, Heinrich | HEINRICH
EISENMANN, IOANNA LETSIOU, ANETTE FEUCHTINGER, WOLFGANG BEISKER, ERNST MANNWEILER, PETER HUTZLER, AND PATRIK ARNZ |
National Research Center for Environment and Health | ||||||||||||||||||
| Intraislet Endothelial Cells Contribute To Revascularization Of Transplanted Pancreatic Islets | 372 | Pancreatic
islet transplantation is an emerging therapy for type 1 diabetes. To survive and function, transplanted islets must revascularize because islet isolation severs arterial and venous connections; the current paradigm is that islet revascularizati |
transplanted pancreatic islets, type 1 diabetes, revascularization | Islets were
isolated from Flk-1wt/wt mice by dissection of the splenic portion of the pancreas followed by collagenase P digestion (Roche Molecular Biochemicals, Indianapolis, IN) as previously described (20). To increase the yield of islets isolated f |
Diabetes | 53 | 2004 | May | 5 | P35G-0-14-C | INTRAISLET | Brissova, Marcela | Marcela Brissova, Michael Fowler, Peter Wiebe, Alena Shostak, Masakazu Shiota, Aramandla Radhika, P. Charles Lin, Maureen Gannon, and Alvin C. Powers | Vanderbilt University | ||||||||||||
| Inverted Colloidal Crystals As Three-Dimensional Microenvironments For Cellular Co-Cultures | 522 | Cellular scaffolds made on the basis of inverted colloidal crystals (ICC) provide a unique system for investigation of cell–cell interactions and their mathematical description due to highly controllable and ordered 3D geometry. Here, we describe three ne | colloidal crystals, cell–cell interactions, layer-by-layer assembly, cell adhesion | Scaffolds were carefully removed from the culture vessel together with the medium and moved to a glass bottom culture dish (MatTek Corporation). The scaffolds were kept immersed in the medium during the process. Co-cultured scaffolds were investigated uti | Journal of Materials Chemistry | 16 | 2006 | 35 | HUMAN | THYMUS | Fibroblast | HFK | Lee, Jungwoo | Jungwoo Lee, Sachin Shanbhag and Nicholas A. Kotov | University of Michigan | |||||||||||
| Isoform
Specificity of PKC Translocation in Living Aplysia Sensory Neurons and a Role for Ca2-Dependent PKC APL I in the Induction of Intermediate-Term Facilitation |
651 | Protein kinase
Cs (PKCs) are important effectors of synaptic plasticity. In Aplysia, there
are two major phorbol ester-activated PKCs, Ca2-activated PKC Apl I and Ca2-independent PKC Apl II. Functional Apl II, but not Apl I, in sensory neurons is requir |
synapses, synaptic transmission, synaptic plasticity, protein kinase C, Aplysia, learning | Plasmid
construction. The pNEX3 enhanced green fluorescent protein (eGFP) PKC Apl I and eGFP PKC Apl II as well as the dominant negatives have been described previously (Manseau et al., 2001). To construct monocistronic red fluorescent protein (mRFP) PKC |
Journal of Neuroscience | 26 | 2006 | August | 34 | 35 mm | Aplysia californica | motor neurons | Sf9 | Zhao, Yali | Yali Zhao,, Karina Leal,,* Carole Abi-Farah,4* Kelsey C. Martin,, Wayne S. Sossin,4 and Marc Klein, | University of
California at Los Angeles |
||||||||||
| Kinetics Of Core Histones In Living Human Cells: Little Exchange Of H3 And H4 And Some Rapid Exchange Of H2B | 577 | Histones H2A
and H2B form part of the same nucleosomal structure as H3 and H4. Stable HeLa cell lines expressing histones H2B, H3, and H4 tagged with green fluorescent protein (GFP) were established; the tagged molecules were assembled into nucleosome |
cell fusion, FRAP, histone actetylation, nucleosome, transcription | General
procedures for fixation, immunolabeling, and image collection using an MRC1000 confocal microscope have been described (Kimura et al., 1999; Manders et al., 1999). Cells grown on glass-bottomed dishes (Mat-Tek) in 100 ng/ml Hoechst 33342 (Harag |
HUMAN | cervix | IZSBS BS TCL20 Morphology: epithelial-like Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology |
epithelial-like | HeLa | Kimura, Hiroshi | Hiroshi Kimura and Peter R. Cook | University of Oxford | ||||||||||||||
| Lateral Membrane Biogenesis In Human Bronchial Epithelial Cells Requires 190-Kda Ankyrin-G | 564 | Ankyrin-G
polypeptides are required for restriction of voltage-gated sodium channels, L1 cell adhesion molecules, and IV spectrin to axon initial segments and are believed to couple the Na/K-ATPase to the spectrin-actin network at the lateral membra |
190-kDa Ankyrin-G, lateral plasma membrane, mitosis, ankyrin | Human
bronchial epithelial cells were a gift of Dr. Peter Mohler. The cells were grown in Dulbecco’s modified Eagle’s medium (Invitrogen) supplemented with 10% fetal bovine serum (Sigma). Cells were trypsinized, and 60,000 cells were plated on 1.4-mm |
HUMAN | BRONCHIAL | Epithelial | Kizhatil, Krishnakumar | Krishnakumar Kizhatil and Vann Bennett | HHMI | ||||||||||||||||
| Live Imaging Of Endogenous Rna Reveals A Diffusion And Entrapment Mechanism For Nanos Mrna Localization In Drosophila | 251 | Background: Localization of nanos mRNA to the posterior pole of the Drosophila embryo directs local synthesis of Nanos protein that is essential for patterning of the anterior-posterior body axis and germ cell function. While nanos RNA is synthesized by t | An individual egg chamber was then mounted on an uncoated #1.5 glass bottom culture dish (MatTek) in 200 µl Schneider's medium. Slight back pressure was applied by laying a 4 mm2 square #1 glass coverslip (Corning) on top of the egg chamber with Dumont #5 | Current Biology | 13 | 14 | EGG | EGG CHAMBERS | Forrest, K.M. | Forrest, K.M., and Gavis, E.R., Department of Molecular Biology, Princeton University, Princeton, NJ, USA. Current Biology, , (4-5), 59-68, (00) | Princeton University | |||||||||||||||
| Loss Of The Homotypic Fusion And Vacuole Protein Sorting Or Golgi-Associated Retrograde Protein Vesicle Tethering Complexes Results In Gentamicin Sensitivity In The Yeast Saccharomyces Cerevisiae | 441 | Gentamicin
continues to be a primary antibiotic against gram-negative infections.
Unfortunately, associated nephro- and ototoxicity limit its use. Our previous mammalian studies showed that gentamicin is trafficked to the endoplasmic reticulum in a retr |
Gentamicin,
toxicity, Golgi-associated retrograde protein (GARP), aminoglycoside, yeast Saccharomyces cerevisiae |
TR was
conjugated to gentamicin as previously described (32). Yeasts were incubated with TR-gentamicin or lucifer yellow (LY) for the specified period of time under the same growth conditions as those used for the growth assay. LY accumulates in the ye |
Antimicrobial Agents and Chemotherapy | 50 | 2006 | February | 2 | kidney | TR | Wagner, Mark C. | Mark C. Wagner, Elizabeth E. Molnar, Bruce A. Molitoris, and Mark G. Goebl | Indiana Center for Biological Microscopy | ||||||||||||
| Missense Mutations In The Globular Tail Of Myosin-Va In Dilute Mice Partially Impair Binding Of Slac2-A/Melanophilin | 432 | The well-known
coat-color mutant mouse dilute exhibits a defect in melanosome transport, and although various mutations in the myosin-Va gene, which encodes an actinbased motor protein, have been identified in dilute mice, why missense mutations in th |
Slac2-a/melanophilin,
Myosin-Va, Rab27A, Melanosome transport, Griscelli syndrome |
Cells
of a murine immortalized melanocyte cell line, melan-a cells (generous gift of Dorothy C. Bennett, St George’s Hospital Medical School, London, UK and Katsuhiko Tsukamoto, University of Yamanashi, Yamanashi, Japan), were cultured on glass-bottom |
Journal of Cell Science | 117 | 2004 | February | 4 | MONKEY, AFRICAN GREEN | KIDNEY | IZSBS BS CL103 Morphology: fibroblast-like Species: monkey, African green; Tissue: kidney; Transformed by: SV40; Original line: CV-1 Properties: virus replication |
fibroblast-like | COS-7 | Fukuda, Mitsunori | Mitsunori Fukuda and Taruho S. Kuroda | RIKEN | |||||||||
| Mitochondrial Swelling Impairs the Transport of Organelles in Cerebellar Granule Neurons | 664 | Organelle
transport in neuronal processes is central to the organization, developmental fate, and functions of neurons. Organelles must be transported through the slender, highly branched neuronal processes, making the axonal transport vulnerable to any p |
The cell
suspension was plated at a density of 1.0
106 on poly-L-lysinecoated MatTek glass bottom culture dishes (2 ml |
P35G-0-14-C | Wistar rat pups | cerebelli | cerebelli | Kaasik | Allen Kaasik, Dzhamilja Safiulina, Vinay Choubey, Malle Kuum, Alexander Zharkovsky, and Vladimir Veksler | University of Tartu, Universite´ Paris-Sud, | ||||||||||||||||
| Multi-parameter
analysis of the kinetics of NF-kB signalling and transcription in single living cells |
649 | Proteins of the
NF-kB transcription factor family normally reside in the cytoplasm of cells in a complex with IkB inhibitor proteins. Stimulation with TNFa leads to proteosomal degradation of the IkB proteins and nuclear translocation of the NF-kB protei |
NF-kB, Signal
transduction, Fluorescent protein fusions, Firefly luciferase, Luminescence imaging, Confocal microscopy |
For
confocal microscopy and fluorescence microscopy, cells were plated on 35 mm Mattek dishes (Mattek, USA) at 2.4´104 cells per plate in 2 ml medium. |
Journal of Cell Science | 115 | 2002 | March | 6 | 35 mm | Hela | Glyn Nelson | Glyn Nelson,
Luminita Paraoan, David G. Spiller, Geraint J. C. Wilde, Mark A. Browne,
Peter K. Djali,, John F. Unitt, Elaine Sullivan, Eike Floettmann and Michael R. H. White,* |
University of Liverpool, Crown Street, Liverpool, L69 7ZB, UK | ||||||||||||
| Mutant Protein Kinase C Gamma Found In Spinocerebellar Ataxia Type 14 Is Susceptible To Aggregate And Cause Cell Death | 630 | Spinocerebellar ataxia type 14 (SCA14) is an autosomal dominant neurodegenerative disease characterized by various symptoms including cerebellar ataxia. Recently, several missense mutations in protein kinase C ( PKC) gene have been found in different SCA | Spinocerebellar ataxia type 14 (SCA14), protein kinase C ( PKC) gene, aggregate, cytoplasm, cell death, cytotoxicity | CHO cells (1×105 cells) were spread onto poly-D-lysine-coated glass bottom culture dishes (MatTek Corp., Ashland, MA) and were transfected with 2.5 μg of plasmid by lipofection. | Journal of Biological Chemistry | 280 | 2005 | August | 32 | chinese hamster | ovary | CHO | Seki, Takahiro | Takahiro Seki, Naoko Adachi, Yoshitaka Ono, Hideki Mochizuki, Keiko Hiramoto, Taku Amano, Hiroaki Matsubayashi, Masayasu Matsumoto, Hideshi Kawakami, Naoaki Saito and Norio Sakai | Hiroshima University | |||||||||||
| Myosin Xva Localizes To The Tips Of Inner Ear Sensory Cell Stereocilia And Is Essential For Staircase Formation Of The Hair Bundle | 408 | Mutations of the gene encoding unconventional myosin XVa are associated with sensorineural deafness in humans (DFNB3) and shaker (Myo15sh2) mice. In deaf Myo15sh2/sh2 mice, stereocilia are short, nearly equal in length, and lack myosin XVa immunoreactivit | stereocilia, hair bundle, myosin Xva, deafness | Inner ear sensory epithelium cultures were prepared from OC, saccule, utricle, and ampule of P3–P5 C57BL 6 mice and from OC of P3 rats. The OC spiral was dissected away from the modiolus in Leibowitz cell culture medium (Invitrogen). The apical and middle |
PNAS | 100 | 2003 | November | 24 | MONKEY, AFRICAN GREEN | KIDNEY | IZSBS BS CL103 | fibroblast-like | COS-7 | Belyantseva, Inna A. | Inna A. Belyantseva, Erich T. Boger, and Thomas B. Friedman | National Institutes of Health | |||||||||
| Nitric Oxide And Calcium Ions In Apoptotic Esophageal Carcinoma Cells Induced By Arsenite | 472 | To
Quantitatively analyze the nitri oxide (NO) and Ca2+ in apoptosis of esophageal carcinoma cells induced by arsenic trioxide (As2O3). The cell line SHEEC1, a malignant esophageal epithelial cell induced by HPV in synergy with TPA in our laboratory, |
Nitric oxide, calcium ions, arsenite, carcinogenic agent, apoptosis | The cells were
cultured on the coverslips within the glass bottom of a small cultured dish (No. 0, uncoated, and irradiated. MatTek Co., USA). At the exponential growth period, the cells were stained with 10µmol·L-1 fluo-3/AM (Molecular Probe) for 30mi |
World Journal of Gastroenterology | 8 | 2002 | February | 1 | HUMAN | ESOPHAGEAL | EPITHELIAL | SHEEC1 | Shen, Zhong-Ying | Zhong-Ying Shen, Wen-Ying Shen, Ming-Hua Chen, Jian Shen, Wei-Jie Cai, Zeng Yi | Shantou University | ||||||||||
| Novel Fluorescence-Based Approaches For The Study Of Biogenic Amine Transporter Localization, Activity, And Regulation | 431 | Pre-synaptic
norepinephrine (NE) and dopamine (DA) transporters (NET and DAT) terminate
catecholamine synaptic transmission through reuptake of released neurotransmitter. Recent studies reveal that NET and DAT are tightly regulated by receptor and second |
Catecholamine; Dopamine; Norepinephrine; ASP+; Quantum dots | To assess
specific labeling of SA-conjugated nanocrystals, we use HEK293 cells that stably express the cloned hNET transporter (Galli et al., 1995). The cell culture is performed as described in Section 2.2.1. To assess the specific labeling of the An |
Journal of Neuroscience Methods | 143 | 2005 | April | 1 | HUMAN | KIDNEY, EMBRYONIC | DSMZ ACC 305 Morphology: adherent fibroblastoid cells growing as monolayer human embryonal kidney established from a human primary embryonal kidney transformed by adenovirus type 5 (Ad 5); classified as risk category 1 according to the German Central Comm |
epithelial | HEK-293 | Mason, J. N. | J.N. Mason, H.
Farmer, I.D. Tomlinson, J.W. Schwartz, V. Savchenko, L.J. DeFelice, S.J. Rosenthal, R.D. Blakely |
Vanderbilt University | |||||||||
| Nuclear import of HIV-1 intracellular reverse transcription complexes is mediated by importin 7 | 565 | Human
immunode®ciency virus type 1 (HIV-1), like other lentiviruses, can infect non-dividing cells. This property depends on the active nuclear import of its intracellular reverse transcription complex (RTC). We have studied nuclear import of puri®ed |
HIV-1,
importins, nuclear import, reverse transcription complex, small interfering RNA |
HeLa and 293T
cells were grown in Dulbecco's modi®ed Eagle's medium (Gibco Laboratories, Paisley, UK) supplemented with 10% fetal calf serum (Helena Bioscience, Newcastle, UK) and 2 mM glutamine at 37°C in a humidi®ed atmosphere containing 5% CO2. Huma |
HUMAN | cervix | IZSBS BS TCL20 Morphology: epithelial-like Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology |
epithelial-like | HeLa | Fassati, Ariberto | Ariberto
Fassati, Dirk GoÈ rlich, Ian Harrison, Lyubov Zaytseva and JoseÂ-Manuel Mingot |
University College | ||||||||||||||
| Nuclear Localization of the 82-kDa Form of Human Choline Acetyltransferase | 626 | Choline acetyltransferase is the enzyme catalyzing synthesis of the neurotransmitter acetylcholine in cholinergic neurons. In human, transcripts encoding two forms of the enzyme with apparent molecular masses of 69 and 82 kDa are found in brain and spinal | Choline acetyltransferase, enzyme catalyzing synthesis, brain, spinal cord, amino acid, cell nucleus | For confocal microscopy, cells were replated at 24 h after transfection onto 35-mm dishes containing a 1-cm glass-bottomed center well (Plastek Cultureware, Ashland, MA) in growth medium supplemented with 20 mM HEPES buffer, pH 7.4. | Journal of Biological Chemistry | 274 | 1999 | July | 27 | P35, 1cm | HEK-293 | Resendes, Manuel C. | Manuel C. Resendes, Tomas Dobransky, Stephen S. G. Ferguson, R. Jane Rylett | University of Western Ontario | ||||||||||||
| Overexpression Of Mitochondrial Manganese Superoxide Dismutase Protects Against Radiation-Induced Cell Death In The Human Hepatocellular Carcinoma Cell Line Hle | 328 | We investigated the potential role of mitochondrial manganese superoxide dismutase (Mn-SOD) in protective activity against irradiation by analyzing cell viability by a colony formation assay and by detecting apoptosis in stably human Mn-SOD gene-transfect | "Cells grown on glass-bottomed (35-mm) dishes (MatTek Corp., Ashland, MA) were stained with Hoechst 33342 fluorescent dye (Molecular Probes, Eugene, OR). … Glass-bottomed (35-mm) dishes (MatTek Corp.) with monolayers were prepared for staining with DAF-FM | Cancer Research | 61 | 2001 | July | 14 | human | LIVER | HEP | Motoori6, S. | Motoori6, S., Majima, H., Ebara4, M., Kato, H., Hirai, 4, F., Kakinuma, S., Yamaguchi, C., Ozawa, T., Nagano7, T., Tsujii, H., and Saisho4, H., Research Center of Charged Particle Therapy, International Space Radiation Laboratory, Redox Resear | Chiba University School of Medicine | ||||||||||||
| Overexpression Of The Human Mnb/Dyrk1A Gene Induces Formation Of Multinucleate Cells Through Overduplication Of The Centrosome | 396 | Previously we cloned the human MNB/DYRK1A gene from the "Down syndrome critical region" on chromosome 21. This gene encodes a dual specificity protein kinase that catalyzes its autophosphorylation on serine/threonine and tyrosine residues. But, the functi | overduplication, chromosome 21, autophosphorylation, Down syndrome | The
procedure for preparation of fluorescently-stained living cells for microscopic observation was described previously [34,35]. Briefly, HeLa cells were plated on a 35-mm glass-bottom culture dish (MatTek Corp., Ashland, MA) and cultured for 1 day in a |
BioMed Central- Cell Biology | 4 | 2003 | HUMAN | cervix | IZSBS BS TCL20 Morphology: epithelial-like Species: human, Black female 31 years old; Tissue: cervix; Tumor: carcinoma, epitheloid Properties: antitumour testing; transformation; tumorigenicity; cytotoxicity; cell biology; bacterial invasiveness; virology |
epithelial-like | HeLa | Funakoshi, Eishi | Eishi Funakoshi, Takamitsu Hori, Tokuko Haraguchi, Yasushi Hiraoka, Jun Kudoh, Nobuyoshi Shimizu and Fumiaki Ito | Setsunan University | |||||||||||
| Planktonic Replication Is Essential For Biofilm Formation By Legionella Pneumophila In A Complex Medium Under Static And Dynamic Flow Conditions | 505 | Legionella
pneumophila persists for a long time in aquatic habitats, where the bacteria
associate with biofilms and replicate within protozoan predators. While L. pneumophila serves as a paradigm for intracellular growth within protozoa, it is less clea |
Planktonic Replication, Legionella pneumophila, protozoa, axenic replication, planktonic bacteria | The architecture
of GFP- and DsRedlabeled L. pneumophila biofilms was analyzed in glass-bottom microwell dishes (35-mm petri dishes; MatTek) under static conditions (no medium replacement) or quasi-static conditions (medium replaced twice a day). An in |
MOUSE | fibroblast; methylcholanthrene | Morphology: fibroblast Species: mouse; Tissue: fibroblast; methylcholanthrene Properties: chromatin conformation; nuclear matrix |
FIBROBLAST | L | Mampel | Jo¨rg Mampel,
Thomas Spirig, Stefan S. Weber, Janus A. J. Haagensen, Søren Molin, and Hubert Hilbi |
Swiss Federal Institute of Technology | ||||||||||||||
| Polarized Expression Of Members Of The Solute Carrier Slc19A Gene Family Of Water-Soluble Multivitamin Transporters: Implications For Physiological Function | 332 | Humans lack biochemical pathways for the synthesis of the micro-nutrients thiamine and folate. Cellular requirements are met through membrane transport activity, which is mediated by proteins of the SLC19A gene family. By using live-cell confocal imaging | folate, polarity, thiamine, transport, vitamin | For transient transfections on cover-glasses, cells were grown to 90% confluency on sterile glass-bottomed Petri dishes (MatTek, MA, U.S.A.) and incubated with 1 mg of plasmid DNA and LIPOFECTAMINETM 2000 in OPTIMEM (Invitrogen). | Biochemical Journal | 376 | 2003 | November | 1 | DOG, COCKER SPANIEL FEMALE | KIDNEY | IZSBS BS CL 64 Morphology: epithelial-like Species: dog, cocker spaniel female; Tissue: kidney; Validated by isoenzymes: confirmed as dog with NP, G6D, MD, MPI, AST, PEPB Depositor: obtained from Flow Laboratories, Irvine (GB) Properties: expressing xenob |
epithelial-like | MDCK | Boulware, Mj. | Boulware, MJ., Subramanian, VS., Said, HM, Marchant, JS. Department of Pharmacology, University of Minnesota Medical School, MN, U.S.A., Department of Medicine and Physiology, University of California, Irvine, CA, U.S.A. and VA Medical Center, Long | University of Minnesota | |||||||||
| Protein Kinase C D And E Mediate Positive Inotropy In Adult Ventricular Myocytes | 530 | To examine
cardiac contractile regulation and protein kinase C (PKC) translocation in
parallel, the d and e isoforms of PKC were fused to green fluorescent protein (GFP) and expressed in adult rat ventricular myocytes maintained in short term culture. PK |
PKC-d; PKC-e; Golgi; Sarcolemma; Adenovirus; Fluorescent proteins | HEK 293 cells
and cultured myocytes expressing fluorescent PKCs were plated onto glass-bottom culture dishes from MatTek Corp. (Ashland, MA) and cultured for 30–40 h before observation. Before imaging cells were washed twice with serum-free culture med |
American Journal of Physiology- Endocrinology and Metabolism | 287 | 2004 | December | 6 | MONKEY, AFRICAN GREEN | KIDNEY | IZSBS BS CL103 Morphology: fibroblast-like Species: monkey, African green; Tissue: kidney; Transformed by: SV40; Original line: CV-1 Properties: virus replication |
fibroblast-like | COS-7 | Kang, Misuk | Misuk Kang, JefferyW.Walker | University of Wisconsin at Madison | |||||||||
| Pseudorabies Virus Us9 Directs Axonal Sorting of Viral Capsids | 911 | Pseudorabies
virus (PRV) mutants lacking the Us9 gene cannot spread from presynaptic to
postsynaptic neurons in the rat visual system, although retrograde spread remains unaffected. We sought to recapitulate these findings in vitro using the isolator cham |
Neurons were
cultured on MatTek Corp. glass-bottom dishes. The dish was warmed to 37°C by using a DH40i Micro-Incubation System (Warner Instrument Corp.) run at constant voltage (5.5 V). |
Journal of Virology | 81 | 2007 | October | 20 | glass bottom dishes | PK15 | M. G. Lyman | M. G. Lyman, B. Feierbach, D. Curanovic, M. Bisher, and L. W. Enquist | ||||||||||||||
| Rab11A And Myosin Vb Are Required For Bile Canalicular Formation In Wif-B9 Cells | 420 | Hepatocytes
polarize by forming functionally distinct sinusoidal (basolateral) and canalicular (apical) plasma membrane domains. Two distinct routes are used for delivery of membrane proteins to the canaliculus. Proteins having glycosylphosphatidylinos |
hepatocyte polarization, plasma membrane segregation | WIF-B9 cells
were cultured in a humidified 7% CO2 incubator at 37°C (14). Unless otherwise specified, cells were plated on a 35-mm glass bottom dish (MatTek) at 1.4 105 cells per dish in complete medium. Infections with recombinant adenoviruses enco |
PNAS | 102 | 2005 | October | 42 | WIF-B9 | Wakabayashi, Yoshiyuki | Yoshiyuki Wakabayashi*‡, Parmesh Dutt, Jennifer Lippincott-Schwartz*, and Irwin M. Arias | National Institutes of Health | |||||||||||||
| Rap1
up-regulation and activation on plasma membrane regulates T cell adhesion |
648 | ap1 and Ras are
closely related GTPases that share some effectors but have distinct functions. We studied the subcellular localization of Rap1 and its sites of activation in living cells. Both GFP-tagged Rap1 and endogenous Rap1 were localized to the plas |
Cells to be
examined by fluorescence microscopy were plated at 2 105 per plate into 35-mm dishes containing a glass coverslipcovered 15-mm cutout (MatTek) and transfected the next day. |
Journal of Cell Biology | 164 | 2004 | February | 3 | 35-mm dishes
containing a glass coverslip 15-mm cutout (MatTek |
COS-1 and MDCK | Bivona, Trever G. | Trever G.
Bivona, Heidi H. Wiener, Ian M. Ahearn, Joseph Silletti, Vi K. Chiu, and Mark R. Philips ,, |
New York University School of Medicine, | |||||||||||||
| Rapid Suppression Of Free Radical Formation By Nerve Growth Factor Involves The Mitogen-Activated Protein Kinase Pathway | 574 | Neurotrophins
such as nerve growth factor (NGF) regulate neuronal survival during development and are neuroprotective in certain models of injury to both the peripheral and the central nervous system. Although many effects of neurotrophins involve lon |
nerve growth factor, protein kinase pathway, neurotrophins, neuronal death | For imaging,
suspensions of GT1-1 or GT1-1 trk cells were diluted and plated onto 35-mm culture dishes (Mat-Tek, Ashland, MA) possessing an oval cut-out sealed by a glass coverslip coated with poly-D-lysine: laminin (12). Cultures were analyzed when , |
GT1-1 | Dugan, Laura L. | LAURA L. DUGAN, DOUGLAS J. CREEDON, EUGENE M. JOHNSON, JR., AND DAVID M. HOLTZMAN | Washington University | ||||||||||||||||||
| Real Time Fluorescence Imaging Of Plctranslocation And Its Interaction With The Epidermal Growth Factor Receptor | 436 | The
translocation of fluorescently tagged PLC g and requirements for this process in cells stimulated with EGF were analyzed using real time fluorescence microscopy applied for the first time to monitor growth factor receptor–effector interactions. |
PLC, EGF receptor, translocation, real time imaging, SH2 and PH domains | A431 and COS-7
cells were cultured in DME supplemented with 10% FBS. The transfection of GFP or RFP fusion protein constructs were made using cells in a 60-mm dish (Nunc), a glass-bottomed 50-mm dish (MatTek Corporation), or on 22-mm-diameter coverslip |
HUMAN | skin | Morphology:
epithelial-like Species: human female 85 years old; Tissue: skin; Tumor: carcinoma, squamous cell Properties: EGF receptor expression; growth factors; cell cycle Available in the following LABORATORY: |
epithelial-like | A431 | Matsuda, Miho | Miho Matsuda,
Hugh F. Paterson, Rosie Rodriguez, Amanda C. Fensome, Moira V. Ellis, Karl Swann, and Matilda Katan |
The Institute of Cancer Research | ||||||||||||||
| Real-Time Optical Monitoring Of Ligand-Mediated Internalization Of A1B-Adrenoceptor With Green Fluorescent Protein | 445 | The study of G
protein-coupled receptor signal transduction and behavior in living cells is technically difficult because of a lack of useful biological reagents. We show here that a fully functional a1b- adrenoceptor tagged with the green fluorescent |
agonist-promoted a1bAR/GFP redistribution, a1-Adrenoceptors, phospholipase C, Green fluorescent protein |
aT3 cells stably
expressing wild-type a1bAR and a1bAR/GFP were seeded at 1 3 105 per well of the cover glass-bottom culture dish (MatTek Corp., Ashland, MA) in 2.0 ml of medium and examined using LSM-GB200 within 30 min at room temperature. |
Molecular Endocrinology | 12 | 1998 | August | 8 | MOUSE | AT3 | Awaji, Takeo | Takeo Awaji,
Akira Hirasawa, Masakazu Kataoka, Hitomi Shinoura, Yasuhisa Nakayama, Tatsuo Sugawara, Shun-ichiro Izumi, and Gozoh Tsujimoto |
National Children's Medical Research Center | ||||||||||||
| Recycling Cd1D1 Molecules Present Endogenous Antigens Processed In An Endocytic Compartment To Nkt Cells | 452 | Mouse CD1d1
molecules present endogenous glycolipids to NKT cells. Although glycolipid
presentation requires CD1d1 transport through the endocytic pathway, the processing requirements for such endogenous Ag presentation by CD1d1 molecules are undefined. |
Endogenous Antigens, CD1d1 molecules, pH, glycolipid | P13.9 cells were
plated in sterile glass-bottom 35-mm dishes coated with poly(D-lysine) (MatTek, Ashland, MA) at a density of 1 107 cells/dish. After overnight adherence, the cells were infected with VV-CD1d1WT, VV-CD1d1TD, or VV-CD1d1Y322A at an MOI |
P13.9 | Roberts, Tonya J. | Tonya J.
Roberts, Venkataraman Sriram, Philip M. Spence, Ming Gui, Kyoko
Hayakawa, Igor Bacik, Jack R. Bennink, Jonathan W. Yewdell, and Randy R. Brutkiewicz |
Indiana University | ||||||||||||||||||
| Recycling Endosomes Can Serve As Intermediates During Transport From The Golgi To The Plasma Membrane Of Mdck Cells | 526 | The AP-1B
clathrin adaptor complex is responsible for the polarized transport of many basolateral membrane proteins in epithelial cells. Localization of AP-1B to recycling endosomes (REs) along with other components (exocyst subunits and Rab8) involve |
Recycling
endosomes, transport, AP-1B clathrin adaptor complex, vesicular stomatitis
virus glycoprotein G |
Coverslip-grown
MDCKT cells were infected with ts045 VSV-G-YFP and induced with 5 mM butyrate for 16 h at 40C. Tfn uptake was performed as described above. Cells were incubated 2 h in media at 20C (last hour in media plus 0.1 mg/ml CHX). Coverslips w |
Journal of Cell Biology | 167 | 2004 | November | 3 | DOG, COCKER SPANIEL FEMALE | KIDNEY | MDCk | Ang, Agnes Lee | Agnes Lee Ang,
Tomohiko Taguchi, Stephen Francis, Heike Fölsch, Lindsay J. Murrells, Marc
Pypaert, Graham Warren, and Ira Mellman |
Yale University | |||||||||||
| Regulation Of SP2 DNA-Binding Activity And trans-Activation | 618 | Regulated transcription requires the collaboration of a variety of transcription factors, including sequence-specific DNA-binding proteins. The Sp-family of DNA-binding proteins governs the expression of a wide variety of mammalian genes, including housek | transcription, Sp-family of DNA-binding proteins, trans-activation | For confocal microscopy, 2-4 x 104 COS-1 cells were plated in 35 mm glass-bottom dishes (MatTek Corp., Ashland, MA) and cultured overnight at 37 °C in DMEM supplemented with heat-inactivated FBS, and 50 μg/ml Pipracil at 37 °C under 5% CO2. | Electronic Thesis- NCSU | COS-1 | Moorefield, K. Scott | K. Scott Moorefield | North Carolina State University | |||||||||||||||||
| Relating Microstructure To Rheology Of A Bundled And Cross-Linked F-Actin Network In Vitro | 369 | The organization of individual actin filaments into higher-order structures is controlled by actin-binding proteins (ABPs). Although the biological significance of the ABPs is well documented, little is known about how bundling and cross-linking quantitat | in vitro, scruin, actin, actin-binding protein, multiparticle tracking | G-actin
solutions were prepared by dissolving lyophilized G-actin in deionized water and dialyzing the solutions against fresh G-buffer (2 mM TrisHCl0.2 mM ATP0.2mMCaCl20.2mMDTT0.005% NaN3, pH8.0) at 4°C for 24 hr; the buffer was replaced with fr |
PNAS | 101 | 2004 | June | 26 | P35G-1.5-14-C | eukaryotic | Shin, J. H. | J. H. Shin, M. L. Gardel, L. Mahadevan, P. Matsudaira, and D. A. Weitz | Massachusettes Institute of Technology | ||||||||||||
| Reversal Of Neuronal Migration In A Mouse Model Of Fetal Alcohol Syndrome By Controlling Second-Messenger Signalings | 550 | The brains of
fetal alcohol syndrome patients exhibit impaired neuronal migration, but
little is known about the mechanisms underlying this abnormality. Here we show that Ca2 signaling and cyclic nucleotide signaling are the central targets of alcohol a |
cerebellar development; granule cell; neuronal cell migration; cAMP; cGMP; rate of cell movement | Cerebella
of postnatal day 0–3 (P0 –P3) mice (CD-1) were placed in ice-chilled HBSS and freed from meninges and choroid plexus (Komuro and Rakic, 1996, 1999; Yacubova and Komuro, 2002a; Kumada and Komuro, 2004). Cerebellar slices were then made with a |
Journal of Neuroscience | 26 | 2006 | January | 3 | esophageal | apoptotic | Kumada, Tatsuro | Tatsuro Kumada, Madepalli K. Lakshmana, and Hitoshi Komuro | The Cleveland Clinic Foundation | ||||||||||||
| Roundabout 2 Regulates Migration Of Sensory Neurons By Signaling In Trans | 575 | Roundabout
(Robo) receptors and their ligand Slit are important regulators of axon
guidance and cell migration. The development of Drosophila em-bryonic sense
organs provides a neuronal migration par-adigm where the in vivo roles of Slit and Robo can be |
Roundabout 2, Migration, Slit, axon guidance, Homothorax | Time-lapse imaging of live embryos was done on an inverted Zeiss LSM5 Pascal with a 20/0.7 n.a. air lens. Live embryos were collected at 29 C to maximize UAS-transgene expression, dechorio-nated in 50% bleach, washed in 0.01% TritonX-100 and water, and s | CHINESE HAMSTER | OVARY | Morphology:
Epithelial Hamster Chinese ovary Depositor: Dr R Downing, PHLS CAMR, Porton Down, Salisbury, UK No restrictions. Patent: None Specified By Depositor Properties: Applications: Nutritional and gene expression studies Available in the following |
epithelial-like | CHO | Kraut, Rachel | Rachel Kraut and Kai Zinn | California Institute of Technology | ||||||||||||||
| Sensitivity Of Planktonic And Biofilm-Associated Salmonella Spp To Ionizing Radiation | 366 | Salmonella
enterica forms biofilms that are relatively resistant to chemical sanitizing
treatments. Ionizing radiation has been used to inactivate Salmonella on a variety of foods and contact surfaces, but the relative efficacy of the process against biof |
ionizing radiation, biofilm-associated bacteria, biofilm | Planktonic and
biofilm-associated cells were prepared in sterile 50-ml centrifuge tubes as follows. For each individual isolate, sterile TSB (5 ml) was inoculated with 200 l of a stock culture and incubated overnight at 37°C to make a fresh culture (a |
Applied and Environmental Microbiology | 71 | 2005 | May | 5 | P35G-1.5-14-C | Salmonella | Niemira, Brendan A. | Brendan A. Niemira and Ethan B. Solomon | US Department of Agriculture | ||||||||||||
| Sonic hedgehog, secreted by amacrine cells, acts as a short-range signal to direct differentiation and lamination in the zebrafish retina | 596 | Neurogenesis in the zebrafish retina occurs in several waves of differentiation. The first neurogenic wave generates ganglion cells and depends on hedgehog (hh) signaling activity. Using transgenic zebrafish embryos that express GFP under the control of t | Sonic hedgehog, Atonal homolog 5, Retina, Differentiation, Neurogenesis, Zebrafish | Embryos were then imbedded in a coverslip-bottomed petri dish (MatTek Corporation), in 0.5% low melting point agarose dissolved in embryo medium with 0.02% tricaine. | Development | 131 | 2004 | August | 16 | |||||||||||||||||