CELL LINE MICROSCOPIC TECHNIQUES HYPERLINK TITLE ABSTRACT MIC CATEGORY PART NO JOURNAL VOLUME ISSUE MONTH YEAR Thickness SPECIES TISSUE ATCC DESC MORPHOLOGY MAIN AUTHOR CONTRIBUTING AUTHORS KEYWORDS MATERIALS & METHODS
3617 Deconvolution 494 Large-Scale Chromatin Decondensation And Recondensation Regulated By Transcription From A Natural Promoter We have examined the relationship between transcription
and chromatin structure using a tandem
array of the mouse mammary tumor virus
(MMTV) promoter driving a
ras
reporter. The array was visualized
as a distinctive fluorescent structure in live cel		     Journal of Cell Biology 154 1 July 2001           Muller, Waltraud G. Waltraud G. Müller, Dawn Walker, Gordon L. Hager, and James G. McNally chromatin decondensation and recondensation, transcription, mouse mammary tumor virus
(MMTV), dipteran flies, Chromosome puffs, lampbrush chromosomes		 To provide assurance that our observations were not affected by imaging
conditions, we used three different methods for time-lapse analysis of
MMTV arrays. First, cells were grown in a Petri dish with a gridded glass
bottom (MatTek Corporation). Partic
3T3 Fluorescence 605 Inhibition of Nuclear Import by the Proapoptotic Protein CC3 We report here that the normal cellular protein CC3/TIP30, when in excess, inhibits nuclear import in vitro and in vivo. CC3 binds directly to the karyopherins of the importin   family in a RanGTP-insensitive manner and associates with nucleoporins in viv     Molecular and Cellular Biology 24 16 August 2004           King, Frank W. Frank W. King and Emma Shtivelman cellular protein CC3/TIP30, nuclear import, transportin, HEAT (huntingtin-elongation A subunit-TOR) 3T3 cells grown in Dulbecco modified Eagle medium plus 10% fetal bovine serum on 4-cm glass bottom plates (MatTek Corp.)…
3T3 Confocal 582 Analysis of nuclear transport signals in the human apurinic/apyrimidinic endonuclease (APE1/Ref1) Themammalianabasic-endonuclease1/redox-factor1 (APE1/Ref1) is an essential protein whose subcellular distributiondependsonthe cellular physiological status. However, its nuclear localization signals have not been studied in detail. We examined nuclear tra     Nucleic Acids Research 33 10 June 2005   mouse     fibroblast-like Jackson, Elias B. Elias B. Jackson, Corey A. Theriot, Ranajoy Chattopadhyay, Sankar Mitra and Tadahide Izumi abasic-endonuclease1/redox-factor1 (APE1/Ref1), reactive oxygen species, gene regulation, nuclear transport, Karyopherin a, amino acid For live cell analysis, cells spread on glass-coated 35 mm dishes (Mattek) were transfected with DNA as described above, and then examined without fixation.
3T3 Inverted Microscope 313 Conventional Kinesin Kif5B Mediates Insulin-Stimulated Glut4 Movements On Microtubules Insulin stimulates glucose uptake in muscle and adipose cells by mobilizing intracellular membrane vesicles containing GLUT4 glucose transporter proteins to the plasma membrane. Here we show in live cultured adipocytes that intracellular membranes contain Inverted Microscope   European Molecular Biology Organization Journal 22 10 May 2003   MOUSE, SWISS ALBINO   IZSBS BS CL 100 Morphology: fibroblast-like
Species: mouse, Swiss albino; Tissue: embryo; Derived from: 3T3-Swiss albino; Validated by isoenzymes: confirmed as mouse with NP, G6PD, PEPB, LD
Depositor: obtained from ATCC
Properties: contact inhibition		 fibroblast-like Park, Semiz, S. Semiz, S., Park, J., Nicoloro, S., Furcinitti, P., Zhang, C., Chawla, A., Leszyk, J., and Czech, M., Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA, USA. EMBO Journal, , (0), 87-99, (00). adipocytes, GLUT4, insulin, kinesin, microtubules 3T3-L1 adipocytes, expressing appropriate cDNA constructs, were seeded in glass-bottomed dishes (MatTek Corporation).
3T3 Fluorescence 237 Effects Of Mitochondrial Uncoupling On Adipocyte Intracellular Ca2+ And Lipid Metabolism Previous data from this laboratory demonstrate that increased intracellular Ca2+ ([Ca2+]i) coordinately regulates human and murine adipocyte lipid metabolism by stimulating lipogenesis and inhibiting lipolysis. However, recent data demonstrate metabolic u     Journal of Nutritional Biochemistry 14 4 April 2003   MOUSE, SWISS ALBINO   IZSBS BS CL 100 Morphology: fibroblast-like
Species: mouse, Swiss albino; Tissue: embryo; Derived from: 3T3-Swiss albino; Validated by isoenzymes: confirmed as mouse with NP, G6PD, PEPB, LD
Depositor: obtained from ATCC
Properties: contact inhibition
Prop		 fibroblast-like Sun, X. Sun, X., and Zemel M.B., University of Tennessee, Knoxville, TN, USA. J. of Nutritional Biochemistry, 4, (4), 9-6 ( 00) y gland; Submandibular gland; Organ culture; Phosphatidylinositol 3-kinase; Phosphatidylinositol 3,4,5-trisphosphate; Akt; Inhibitors; Mouse; Time-lapse microscopy Preadipocytes were plated in 35-mm dishes (P35G-0-14-C, MatTek).
3T3-L1 preadipocytes or COS cells Confocal 877 Assessment of fat-specific protein 27 in the adipocyte lineage suggests a dual
role for FSP27 in adipocyte metabolism and cell death		 Assessment
of fat-specific protein 27 in the adipocyte lineage suggests a dual
role for FSP27 in adipocyte metabolism and cell death. Am J Physiol
Endocrinol Metab 294: E654–E667, 2008. First published January 15,
2008; doi:10.1152/ajpendo.00104.2007.		   glass bottom dishes American Journal of Physiology: Endocrinology and Metabolism 294 4 April 2008           Ji Young Kim Ji Young Kim, Kun Liu, Shengli Zhou, Kristin Tillison, Yu Wu, and Cynthia M. Smas insulin; tumor necrosis factor-; adipogenesis; cell death-inducing
DFF45-like effector; apoptosis; 3T3-L1		 Confocal imaging studies using MitoTracker
Red CMX Ros (Invitrogen) were conducted on live cells by plating
3T3-L1 preadipocytes or COS cells onto laminin-coated MatTek glass
bottom dishes (MatTek, Ashland, MA)
43-3B (CHO) Fluorescence 737 In vivo dynamics of chromatin-associated complex formation in mammalian nucleotide excision repair Chromatin is the substrate for many processes in the cell nucleus, including transcription, replication, and various DNA repair systems,
all of which require the formation of multiprotein machineries
on the chromatin fiber. We have analyzed the kinetics		 Immunofluorescence P35GC-1.5-10-C PNAS 101 45 November 2004   Chinese hamster Ovary     Mone, Martijn J. Martijn J. Mone´ *, Tytus Bernas, Christoffel Dinant*, Feliks A. Goedvree*, Erik M. M. Manders*, Marcel Volker‡,
Adriaan B. Houtsmuller§, Jan H. J. Hoeijmakers, Wim Vermeulen, and Roel van Driel		 DNA repair ERCC1-XPF in vivo kinetics TFIIH Cells were cultured in glass-bottomed Petri dishes (MatTek, Ashland, MA)
and were used at 60–80% confluency.
6-3 Confocal 529 Translocation Of An Endoproteolytically Cleaved Maxi-K Channel Isoform: Mechanisms To Induce Humanmyometrial Cell Repolarization Large conductance Ca2+- and voltage-activated K+ (maxi-K) channels modulate human
myometrial smooth muscle cell (hMSMC) excitability; however, the role of individual
alternatively spliced isoforms remains unclear. We have previously shown that the trans		     American Journal of Physiology- Renal Physiology 289 2 August 2005   RABBIT       Korovkina, Victoria P. Victoria P. Korovkina, Adam M. Brainard and Sarah K. England human
myometrial cell repolarization, endoproteolytic cleavage, N-myristoylation, C-termini, Endoproteolytic digest		 Mouse laminin (Sigma-Aldrich, St Louis, MO, USA) was
used to coat glass-bottom 35mm cell culture dishes
(MatTek Corporation, Ashland, MA, USA). Differentiated
hMSMCs were pre-loaded for 30 min with 2 µm of a
membrane potential-sensitive dye DiBAC4(3) 
6-3 Confocal 508 Superoxide Production At Phagosomal Cup/Phagosome Through I Protein Kinase C During Fc R-Mediated Phagocytosis In Microglia Protein kinase C (PKC) plays a prominent role in immune signaling. To elucidate the signal transduction in a respiratory burst
and isoform-specific function of PKC during Fc R-mediated phagocytosis, we used live, digital fluorescence imaging of mouse
mi		     Journal of Immunology 173 7 October 2004         ENDOTHELIAL Ueyama, Takehiko Takehiko Ueyama, Michelle R. Lennartz, Yukiko Noda, Toshihiro Kobayashi,
Yasuhito Shirai, Kyoko Rikitake, Tomoko Yamasaki, Shigeto Hayashi, Norio Sakai,
Harumichi Seguchi, Makoto Sawada, Hideki Sumimoto, and Naoaki Saito		 Superoxide Production, Phagocytosis, Protein kinase C, O2-  production, Alzheimer’s disease The 6-3 microglial cells are generated by spontaneous immortalization of
primary microglia from op/op mice which are deficient of M-CSF-derived
macrophages and severely monocytopenic (15, 16). The 6-3 cells closely
resemble primary microglia with respe
A431 Confocal 436 Real Time Fluorescence Imaging Of Plctranslocation And Its Interaction With The Epidermal Growth Factor Receptor The translocation of fluorescently tagged
PLC
g
and requirements for this process in cells stimulated
with EGF were analyzed using real time fluorescence
microscopy applied for the first time to monitor
growth factor receptor–effector interactions. 		     Journal of Cell Biology 153 3 April 2001   HUMAN skin Morphology: epithelial-like
Species: human female 85 years old; Tissue: skin; Tumor: carcinoma, squamous cell
Properties: EGF receptor expression; growth factors; cell cycle

Available in the following LABORATORY:		 epithelial-like Matsuda, Miho Miho Matsuda, Hugh F. Paterson, Rosie Rodriguez, Amanda C. Fensome, Moira V. Ellis,
Karl Swann, and Matilda Katan		 PLC,  EGF receptor, translocation, real time imaging,  SH2 and PH domains A431 and COS-7 cells were cultured in DME supplemented with 10%
FBS. The transfection of GFP or RFP fusion protein constructs were
made using cells in a 60-mm dish (Nunc), a glass-bottomed 50-mm dish
(MatTek Corporation), or on 22-mm-diameter coverslip
A431 Inverted Microscope 248 Antiproliferative Effect Of Ca2+ Channel Blockers On Human Epidermoid Carcinoma A431 Cells The effects of Ca2+ channel blockers on the proliferation of human epidermoid carcinoma A431 cells were investigated by microtiter tetrazolium (MTT) proliferation assay and bromodeoxyuridine (BrdU) incorporation assay. Dihydropyridine derivatives, such as Inverted Microscope   European Journal of Pharmacology 472 1-2 July 2003   HUMAN skin Morphology: epithelial-like
Species: human female 85 years old; Tissue: skin; Tumor: carcinoma, squamous cell
Properties: EGF receptor expression; growth factors; cell cycle

Available in the following LABORATORY:		 epithelial-like Yoshida, J. Yoshida, J., Ishibashi, T., and Nishio, M., Department of Pharmacology, Kanazawa Medical University, Uchinada, Ishikawa, Japan. European Journal of Pharmacology, 47, (-), - (00) Ca2+ channel blocker; Proliferation; Human epidermoid carcinoma A431 cell; Intracellular Ca2+; Fura-2; Fluo-3; Amlodipine For the microscopic fluorimetric measurement of intracellular free Ca2+ concentrations ([Ca2+]i), A431 cells were plated on poly-D-lysine coated glass bottom dishes (MatTek, Ashland, USA).
A549 cells and CHO-K1 Fluorescence 701 Role of Adipose Differentiation-Related Protein in Lung Surfactant Abstract Based on data developed with the use of isolated
lipid droplets from neonatal rat lung lipofibroblasts, we
speculated previously that the droplet coat protein, adipose
differentiation-related protein (ADFP), mediated the transfer
of lipids into t		 Immunofluorescence and Confocal  P35G-1.5-10-C Journal of Lipid Research 47 11 November 2006   Human lung carcinoma     Magra Amy L. Magra,,* Pamela S. Mertz,,* John S. Torday, and Constantine Londos lipid droplets . surfactant phospholipid .
PAT proteins		 Glass-bottom 35 mm (diameter) tissue culture dishes were purchased
from MatTek Corp. (Ashland, MA).
A549-s Fluorescence 230 Oxidative Stress Increases Internal Calcium Stores And Reduces A Key Mitochondrial Enzyme Cornell University Medical College at Burke Medical Research Institute, 785 Mamaroneck Avenue, White Plains, NY 10605, USA. ggibson@med.cornell.edu

Fibroblasts from patients with genetic and non-genetic forms of Alzheimer's disease (AD) show many abnor		     Biochimica et Biophysica Acta 1586 2 March 2002         Fibroblast Gibson, G.E. Gibson, G.E., Zhang, H., Xu, H., Park, L., and Jeitner T. M., Cornell University Medical College at Burke Medical Research Institute, White Plains, NY, USA. Biochimica et Biophysica Acta (BBA)/Molecular Basis of Disease, 586, (), 77-89, (00) Alzheimer’s disease; Calcium; Reactive oxygen species; Fibroblast; a-Ketoglutarate dehydrogenase complex; Antioxidant Cells were sub-cultured weekly by trypsinizing and reseeding at 104/cm2. For imaging experiments, fibroblasts were seeded at 500/cm2 on MatTek glass bottom Petri dishes (Ashland, MA, USA) 5–6 days before the experiments.
A7R5 Atomic Force Microscopy  (AFM) 489 Elasticity Of Native And Cross-Linked Polyelectrolyte Multilayer Films Mechanical properties of polyelectrolyte multilayer films were studied by nanoindentation using the atomic
force microscope (AFM). Force-distance measurements using colloidal probe tips were systematically
obtained for supported films of poly(L-lysine) 		     Biomacromolecules 5 5 September 2004   RAT aorta thoracic, embryonic Morphology: fibroblast-like
Species: rat, DB1X; Tissue: aorta thoracic, embryonic
Properties: biochemistry; biophysics

Available in the following LABORATORY:		 fibroblast-like Richert, Ludovic Ludovic Richert, Adam J. Engler, Dennis E. Discher, and Catherine Picart polyelectrolyte multilayer films, Biopolymers, cell
adhesion		 A7r5 smooth muscle cells (SMC) were
maintained in polystyrene flasks between passages 2 and 15,
and cultured in media containing DMEM, supplemented with
10% of fetal bovine serum and antibiotics. Cells were passed
every 3 days, when reaching 80% conf
A7R5 Atomic Force Microscopy  (AFM) 483 Surface Probe Measurements Of The Elasticity Of Sectioned Tissue, Thin Gels And Polyelectrolyte Multilayer Films:Correlations Between Substrate Stiffness And Cell Adhesion Surface probe measurements of the elasticity of thin film matrices as well as biological samples prove generally
important to understanding cell attachment across such systems. To illustrate this, sectioned arteries were probed by
atomic force microscop		     Surface Science 570 1-2 October 2004     MUSCLE     Engler, Adam J. Adam J. Engler, Ludovic Richert, Joyce Y. Wong,
Catherine Picart, Dennis E. Discher		 Surface structure, morphology, roughness, and topography; Adhesion; Atomic force microscopy A7r5 SMCs (aorta-derived cell line) were maintained
in polystyrene flasks between passages 2
and 15, and cultured in media containing DMEM,
supplemented with 10% of fetal bovine serum and
antibiotics. Cells were passed every 3 days, when
reaching 80
adipocyte Time-Lapse 435 Lfa-1/Icam-1 Interaction Lowers The Threshold Of B Cell Activation By Facilitating B Cell Adhesion And Synapse Formation The
integrin LFA-1 and its ligand ICAM-1 mediateB cell adhesion, but their role in membrane-bound antigen recognition
is still unknown. Here, using planar lipid bilayers
and cells expressing ICAM-1 fused to green fluorescence protein, we found that the		 Time-lapse video microscopy   Immunity 20 5 May 2004     LYMPHOCYTE     Carrasco, Yolanda R. Yolanda R. Carrasco, Sebastian J. Fleire,
Thomas Cameron, Michael L. Dustin,
and Facundo D. Batista		 Cell Adhesion, synapse, B cell receptor (BCR) Time-lapse video microscopy was performed using a Nikon Diaphot
200 inverted microscope equipped with a CCD camera (Hama-matsu Orca). ICAM-1-GFP APCs were split on 35 mm glass-bottom dishes (MatTek Corporation, Ashland, MA). The dish was placed on a 37 C
adipocyte Confocal 333 Human Lung Cancer Cells Express Functionally Active Toll-Like Receptor  9 Here we show that TLR9 is expressed in a selection of human lung cancer tissues and various tumor cell lines. The expression of functionally active TLR9 in human malignant tumors might affect treatment approaches using CpG-ODN and shows that malignant cel     Respiratory Research 6 1 January 2005   HUMAN LUNG CARNINOMA DSMZ ACC 107
Morphology: epithelial cells, growing adherently as monolayer
human lung carcinoma established from an explanted lung tumor which was removed from a 58-year-old Caucasian man in 1972; cells were described to induce tumors in athmyic mice and 		 EPITHELIAL Droemann, D. Droemann, D., Albrecht, , D., Gerdes, J., Ulmer, AJ., Branscheid, D., Vollmer4, E., Dalhoff5, K., Zabel,5 , P., Goldmann4, T.  Medical Clinic, Research Center Borstel, Borstel, Germany;  Department of Immunology and Cell Biology, Research Center   A549-cells were seeded in 35-mm glass bottom dishes (MatTek Corp.) overnight. Cells were transfected with GFP-huTLR9 using Polyfect (Qiagen) according to the manufacturer's instructions or incubated in medium.
ALVEOLAR TYPE II Atomic Force Microscopy  (AFM) 426 Novel Dynamic Rheological Behavior Of Individual Focal Adhesions Measured Within Single Cells Using Electromagnetic Pulling Cytometry The rheology of cells and sub-cellular structures, such as focal adhesions, are important for cell form and function. Here we
describe electromagnetic pulling cytometry (EPC), a technique to analyze cell rheology by applying dynamic tensional forces to
     Acta Biomaterialia 1 3 May 2005   BOVINE       Overby, Darryl R. Darryl R. Overby, Benjamin D. Matthews, Eben Alsberg, Donald E. Ingber Cell mechanics; Cytoskeleton; Focal adhesion; Creep; Magnetic Bovine capillary endothelial cells were cultured in
10% CO2 at 37  C on gelatin-coated tissue culture dishes
in Dulbeccos modified Eagles medium (DMEM,
Gibco-BRL) supplemented with 10% calf serum (CS;
Hyclone), 10 mM Hepes (JRH-Biosciences), 2 mM
L
ALVEOLAR TYPE II Inverted Microscope 308 Role Of Clathrin- And Actin-Dependent Endocytotic Pathways In Lung Phospholipid Uptake We evaluated the contribution of endocytotic pathways to pulmonary uptake of surfactant lipids from the alveolar space. Resting and stimulated 8-bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP) uptake of unilamellar liposomes labeled with either [3H] Inverted Microscope   American Journal of Physiololgy- Lung Cellular and Molecular Physiology 284 6 June 2003   RAT LUNG   EPITHELIAL Rückert, P. Rückert, P., Bates, S., and Fisher, A.,  Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania. Am J Physiol Lung Cell Mol Physiology 84, (6),  L98-L989, (00). liposomes; lamellar bodies; alveolar type II cells; perfused rat lung; 1-palmitoyl-2-[12-(7-nitro-2-1,3-benzoxadiazol-4-yl) amino] dodecanoyl-phosphatidylcholine The cells were cultured for 24 h on 35-mm glass-bottomed dishes (MatTek, Ashland, MA) in minimal essential medium containing 10% fetal calf serum in 5% CO2 in air at 37°C.
AM Confocal 587 Subunit composition and role of Na+,K+-ATPases in adrenal chromaffin cells Adrenal medullary (AM) cells are exposed to high concentrations of cortical hormones, one of which is a ouabain-like substance. Thus, the effects of ouabain on catecholamine secretion and distribution of Na+,K+-ATPase α and β subunits in rat and guinea-pi Laser Confocal   Journal of Physiology 564 1 April 2005   guinea-pig       Lin, Hai Hai Lin, Shoichiro Ozaki, Naoji Fujishiro, Kazuo Takeda, Issei Imanaga, Glenn D Prestwich, and Masumi Inoue cortical hormones, amperometry, ouabain After incubation, tissues were washed in Ca2+-deficient solution, and placed in a dish with non-fluorescent glass at the central area (MatTek), then dissociated using fine needles.
AMOEBA Fluorescence 340 Dictyostelium Macroautophagy Mutants Vary In The Severity Of Their Developmental Defects Macroautophagy is the major mechanism that eukaryotes use to recycle cellular components during stressful conditions. We have shown previously that the Atg12-Atg5 conjugation system, required for autophagosome formation in yeast, is necessary for Dictyost     Journal of Biological Chemistry 279 15 April 2004           Otto, G. P. Otto, G. P., Wu, M. Y., Kazgan, N.,  Anderson, O. R., and Kessin , R., Department of Anatomy and Cell Biology, Columbia University, New York, NY and the Department of Biology, Lamont-Doherty Earth Observatory, Columbia University, Palisades, NY. J.   To examine the localization of GFP fusion
proteins, axenic cells were incubated overnight in HL5 medium supplemented
with 10 microg/ml G418 in 35-mm glass bottom microwell dishes
(MatTek Corp.).
amyloid Fluorescence 443 Functional Inactivation Of Immature Dendritic Cells By The Intracellular Parasite Toxoplasma Gondii Despite its noted ability to induce strong cellular immunity, and its known susceptibility to IFN- -dependent immune effector
mechanisms, the protozoan Toxoplasma gondii is a highly successful parasite, able to replicate, disseminate, and either kill the		     Journal of Immunology 173 4 August 2004           Mckee Amy S. McKee, Florence Dzierszinski, Marianne Boes, David S. Roos, and
Edward J. Pearce		 Toxoplasma gondii, parasite, immune DC (2  106/ml) were cultured on sterile glass coverslips in 24-well plates
with or without live or HK tachyzoites, or soluble extracts, for 18 h. Cells
were fixed in 4% paraformaldehyde, permeabilized in saponin buffer, and
stained with biotin-conjuga
Aplysia californica Second Generation Harmonic 888 Optical Recording of Action Potentials with
Second-Harmonic Generation Microscopy		 Nonlinear microscopy has proven to be essential for neuroscience investigations of thick tissue preparations. However, the optical
recording of fast (1 msec) cellular electrical activity has never until now been successfully combined with this imaging m		   glass bottom dishes Journal of Neuroscience 24 4 January 2004           Daniel A. Dombeck Daniel A. Dombeck, Mireille Blanchard-Desce, and Watt W. Webb Imaging; action potential; membrane potential; second-harmonic generation; microscopy; nonlinear; optical; voltagesensitive
dye		 The cultures were grown in glassbottom
dishes (MatTek, Ashland, MA)
apoptotic Confocal 550 Reversal Of Neuronal Migration In A Mouse Model Of Fetal Alcohol Syndrome By Controlling Second-Messenger Signalings The brains of fetal alcohol syndrome patients exhibit impaired neuronal migration, but little is known about the mechanisms underlying
this abnormality. Here we show that Ca2  signaling and cyclic nucleotide signaling are the central targets of alcohol a		     Journal of Neuroscience 26 3 January 2006     esophageal     Kumada, Tatsuro Tatsuro Kumada, Madepalli K. Lakshmana, and Hitoshi Komuro cerebellar development; granule cell; neuronal cell migration; cAMP; cGMP; rate of cell movement Cerebella of
postnatal day 0–3 (P0 –P3) mice (CD-1) were placed in ice-chilled HBSS
and freed from meninges and choroid plexus (Komuro and Rakic, 1996,
1999; Yacubova and Komuro, 2002a; Kumada and Komuro, 2004). Cerebellar
slices were then made with a
Arabidopsis seed Fluorescence 455 Epidermal Growth Factor Protects Rat Epithelial Cells Against Acid-Induced Damage Through The Activation Of Na1/H1 Exchangers We examined the effect of epidermal growth factor (EGF) on
acid-induced cell damage in rat gastric epithelial cells (RGM1)
and investigated the mechanisms of this effect. Cells were
incubated with EGF for 5, 15, 30, and 60 min, and then immersed
in an		 Attofluoro Ratio Vision   Journal of Pharmacology and Experimental Therapeutics 288 2 February 1999   RAT       Furukawa, Osamu OSAMU FURUKAWA, HIROSHI MATSUI, NORIKO SUZUKI and SUSUMU OKABE epidermal growth factor, cell damage, acid Changes in the pHi of
RGM1 cells were detected with an image analyzer (Attofluor Ratio-
Vision, Karl Zeiss Co., Ltd., Obernkochen, Germany). RGM1 cells
cultured in a glass-bottomed culture dish (MatTek Co., Ashland, MA)
were incubated with 0.3 mM BCEC
ARPE-19 Confocal 710 In vitro assays for evaluating the ultraviolet B-induced damage in cultured human retinal pigment epithelial cells The present study demonstrates broadband UV-B-induced damage of cultured human retinal pigment epithelial cells as an effort to
develop an in vitro model that can be used, along with in vivo research and other in vitro efforts, to evaluate the need for re		   Collagen coated glass bottom dishes Journal of Photochemistry and Photobiology 88 1 July 2007   human  retinal   epithelial Youn, Hyun-Yi Hyun-Yi Youn, Vladimir Bantseev, Niels C. Bols, Anthony P. Cullen, Jacob G. Sivak - Not in ACT UVB-induced retinal damage; RPE; Alamar blue assay; Cellular viability; Confocal microscopy; Mitochondrial damage; DNA damage;
Phagocytotic activity		 In the present study,
1 · 105 cells in 1.5 ml of culture medium were seeded into
collagen coated glass bottom culture Petri dishes (MatTek,
MA, USA).
Astrocytics Fluorescence 571 Inhibition Of Map Kinase Activity Moderates Changes In Expression And Function Of Cx32 But Not Claudin-1 During Dna Synthesis In Primary Culturesof Rat Hepatocytes The signal transduction pathways and activation
of the MAP kinase or PI3 kinase signaling cascade regulate
a variety of cellular processes, including proliferation and
differentiation in hepatocytes. To elucidate the mechanisms
of signal transmission 		     Medical Electron Microscopy 37 2 June 2004   RAT       Kojima, Takashi Takashi Kojima, Toshinobu Yamamoto,  Mengdong Lan, Masaki Murata, Ken-ichi Takano,  Mitsuru Go, Shingo Ichimiya, Hideki Chiba, Norimasa Sawada Gap junctions, Tight junctions, Hepatocytes, MAPK, PI3K This procedure was performed according to Balda et al.
(30). Sphyngomyelin/bovine serum albumin (BSA) complexes
(5 nM/ml) were prepared in P buffer (10nM HEPES,
pH 7.4, 1mM sodium pyruvate, 10mM glucose, 3mM CaCl,
and 145mM NaCl) using Bodipy-FL-sphyn
AT3 Confocal 445 Real-Time Optical Monitoring Of Ligand-Mediated Internalization Of A1B-Adrenoceptor With Green Fluorescent Protein The study of G protein-coupled receptor signal
transduction and behavior in living cells is technically
difficult because of a lack of useful biological
reagents. We show here that a fully functional a1b-
adrenoceptor tagged with the green fluorescent		     Molecular Endocrinology 12 8 August 1998   MOUSE       Awaji, Takeo Takeo Awaji, Akira Hirasawa, Masakazu Kataoka,
Hitomi Shinoura, Yasuhisa Nakayama, Tatsuo Sugawara,
Shun-ichiro Izumi, and Gozoh Tsujimoto		 agonist-promoted
a1bAR/GFP redistribution, a1-Adrenoceptors, phospholipase C, Green fluorescent protein		 aT3 cells stably expressing wild-type
a1bAR and a1bAR/GFP were seeded at 1 3 105 per well of the
cover glass-bottom culture dish (MatTek Corp., Ashland, MA)
in 2.0 ml of medium and examined using LSM-GB200 within
30 min at room temperature.
Atb2 Fluorescence 354 The V260I Mutation In Fission Yeast -Tubulin Atb2 Affects Microtubule Dynamics And Eb1-Mal3 Localization And Activates The Bub1 Branch Of The Spindle Checkpoint We have identified a novel temperature-sensitive mutant of fission yeast α-tubulin Atb2 (atb2-983) that contains a single amino acid substitution (V260I). Atb2-983 is incorporated into the microtubules, and their overall structures are not altered noticea   P35G-1.5-10-C Molecular Biology of the Cell 17 3 March 2006 1.5 YEAST       Asakawa, Kazuhide Kazuhide Asakawa, Kazunori Kume, Muneyoshi Kanai, Tetsuya Goshima,
Kohji Miyahara, Susheela Dhut, Wee Wei Tee, Dai Hirata, and Takashi Toda		 amino acid, microtubule, kinetochore, Adenomatous Polyposis Coli (APC) A parental strain, which contains minichromosome Ch16
 and Bub1-GFP was mutagenized with nitrosoguanidine as
described previously, spread on rich YE5S plates and
incubated at 27°C. Colonies were replica-plated onto YE5S at 36°C and YE
plates (rich med
Au565, MCF7, MCF7/HER2, and HMCs Confocal 759 Activation of AMP-activated protein kinase by human EGF receptor 2/EGF receptor tyrosine kinase
inhibitor protects cardiac cells		 The human EGF receptor (HER) 2 receptor tyrosine kinase is a survival factor for human cardiomyocytes, and its inhibition may
explain the increased incidence of cardiomyopathy associated with
the anti-HER2 monoclonal antibody trastuzumab (Genentech,
So		   P35GC-1.0-14-C, P35G-1.0-14-C PNAS 104 25 June 2007   Human Breast cancer, Cardiomyocytes     Spector, Neil L. Neil L. Spector*, Yosef Yarden, Bradley Smith‡, Ljuba Lyass§, Patricia Trusk§, Karen Pry§, Jason E. Hill§, Wenle Xia*,
Rony Seger, and Sarah S. Bacus		 monoclonal antibodies Au565, MCF7, MCF7/HER2, and HMCs cells
were seeded on glass bottom dishes (MatTek, Ashland, MA) the
day before the experiment.
AX2 (wild-type) Fluorescence 831 Dictyostelium Aurora Kinase Has Properties of both Aurora A and
Aurora B Kinases		 Aurora kinases are highly conserved proteins with important roles in mitosis. Metazoans contain two
kinases, Aurora A and B, which contribute distinct functions at the spindle poles and the equatorial region
respectively. It is not currently known wheth		   Glass-Bottom Dishes Eukaryotic Cell 7 5 May 2008           Hui Li Hui Li, Qian Chen, Markus Kaller, Wolfgang Nellen, Ralph Graf, and Arturo De Lozanne Aurora kinase Cell suspension (0.5 ml) was plated on a small petri dish with a coverslip
mounted at the bottom (MatTek, Ashland, MA).
B104 Microinjection 719 The Microtubule-associated Protein Tumor Overexpressed Gene Binds to the RNA Trafficking Protein Heterogeneous
Nuclear Ribonucleoprotein A2		 In neural cells, such as oligodendrocytes and neurons, transport of certain RNAs along microtubules is mediated by the
cis-acting heterogeneous nuclear ribonucleoprotein A2 response element (A2RE) trafficking element and the cognate
trans-acting heterogen		 Microinjection glass bottom dishes Molecular Biology of the Cell 16 4 April 2005   Rat Oligodendrocytes     Kosturko, Linda D. Linda D. Kosturko,* Michael J. Maggipinto,* Chrystal D’Sa, John H. Carson, and Elisa Barbarese*   Microinjection of primary oligodendrocytes plated on glass bottom dishes
(MatTek, Ashland, MA) was performed using an electronic microinjection
system (Eppendorf, Hamburg, Germany).
B104 Microinjection 718 Heterogeneous Nuclear Ribonucleoprotein (hnRNP) E1 Binds to hnRNP A2 and Inhibits Translation of A2
Response Element mRNAs		 Heterogeneous nuclear ribonucleoprotein (hnRNP) A2 is a trans-acting RNA-binding protein that mediates trafficking of
RNAs containing the cis-acting A2 response element (A2RE). Previous work has shown that A2RE RNAs are transported
to myelin in oligodendr		 Microinjection glass bottom dishes Molecular Biology of the Cell 17 8 August 2006   Rats Oligodendrocytes     Kosturko, Linda D. Linda D. Kosturko,* Michael J. Maggipinto,* George Korza, Joo Won Lee, John H. Carson, and Elisa Barbarese*   Microinjection of cells plated on glass-bottomed dishes (MatTek, Ashland,
MA) was performed using an electronic microinjection system (Eppendorf,
Hamburg, Germany).
B104 Microinjection 717 The Microtubule-Associated Protein Tumor Overexpressed Gene/Cytoskeleton-Associated Protein 5 Is Necessary for
Myelin Basic Protein Expression in Oligodendrocytes		 Tumor overexpressed gene (TOG) protein, encoded by cytoskeleton-associated protein CKAP5, is a microtubule-associated protein that
binds to heterogeneous nuclear ribonucleoprotein (hnRNP) A2. hnRNP A2 is an RNA trafficking factor that associates with myel		 Microinjection P35G-1.5-10-C Journal of Neuroscience 27 29 July 2007   Rat Oligodendrocytes     Francone, Victor P. Victor P. Francone, Michael J. Maggipinto, Linda D. Kosturko, and Elisa Barbarese cell culture; differentiation; PCR-based short-hairpin RNA interference; RNA trafficking; translation; fluorescence
microscopy		 Microinjection of cells plated on glass bottom dishes (MatTek, Ashland,
MA)was performed using an electronic microinjection system (Eppendorf,
Hamburg, Germany).
B104 Microinjection 716 The microtubule-associated protein, TOG, binds to the RNA trafficking protein,
hnRNP A2.		 In neural cells, such as oligodendrocytes and neurons, transport of certain RNAs
along microtubules is mediated by the cis-acting A2RE trafficking element and the
cognate trans-acting hnRNP A2 trafficking factor. Using a yeast two-hybrid screen we
have id		 Microinjection glass bottom dishes Molecular Biology of the Cell 16 4 April 2005   Rat primary oligodendrocytes     Kosturko, Linda D. Linda D. Kosturko, Michael J. Maggipinto, Chrystal D’Sa, John H. Carson,, and
Elisa Barbarese,*		 cytoskeleton, heterogeneous nuclear ribonucleoprotein, RNA binding
protein, RNA granules, oligodendrocytes.		 Microinjection of primary oligodendrocytes plated on glass bottom dishes
(MatTek Corp., Ashland, MA) was performed using an electronic microinjection system
(Eppendorf, Hamburg, Germany).
BDCA2 Fluorescence 732 Differential expression of IFN- and TRAILDR5 in lymphoid tissue of progressor versus
nonprogressor HIV-1-infected patients		 Loss of CD4 T cells, the hallmark of HIV pathogenesis, was
suggested to be partly due to apoptosis. We recently reported that
IFN- produced by HIV-1-activated plasmacytoid dendritic cells
(pDCs) contributes to CD4 T cell apoptosis by the TNF-related
ap		 Immunofluorescence poly-L-lysine-coated chamber coverglass PNAS 103 18 May 2006   Human tonsil biopsies     Herbeuval, Jean-Philippe Jean-Philippe Herbeuval*, Jakob Nilsson, Adriano Boasso*, Andrew W. Hardy*, Michael J. Kruhlak*,
Stephanie A. Anderson‡, Matthew J. Dolan§, Michel Dy, Jan Andersson, and Gene M. Shearer		 pDC, IRF-7, MyD88, AT-2 HIV-1 Cells were washed, allowed to adhere to poly-L-lysine-coated chamber
coverglass (MatTek, Ashland, MA), and mounted in staining
buffer (Caltag).
Bergmann Inverted Microscope 493 Pten Deletion In Bergmann Glia Leads To Premature Differentiation And Affects Laminar Organization Development of the central nervous system is controlled by
both intrinsic and extrinsic signals that guide neuronal
migration to form laminae. Although defects in neuronal
mobility have been well documented as a mechanism for
abnormal laminar formatio		 Inverted Microscope glass bottom microwell dishes Development 132 14 July 2005         PRIMARY GRANULE NEURON Qing Yue Qing Yue, Matthias Groszer, Jose S. Gil, Arnold J. Berk, Albee Messing, Hong Wu and Xin Liu PTEN, Bergmann glia, Cerebellar development, Mouse Cerebellar cells were purified by using a modified procedure
described by Hatten (Hatten, 1985). Cerebella were dissected from P6
animals. After removal of meninges, cerebellar tissue was treated with
Trypsin-EDTA (Gibco-BRL) and triturated into single
BHK Inverted Microscope 312 Nfat Functions As A Working Memory Of Ca2+ Signals In Decoding Ca2+ Oscillation Transcription by the nuclear factor of activated T cells (NFAT) is regulated by the frequency of Ca2+ oscillation. However, why and how Ca2+ oscillation regulates NFAT activity remain elusive. NFAT is dephosphorylated by Ca2+-dependent phosphatase calcine inverted microscope   European Molecular Biology Organization Journal 22 22 November 2003   CHINESE HAMSTER, NEWBORN KIDNEY   fibroblastic Tomida, T. Tomida, T., Hirose, K., Takizawa, A., Shibasaki F., and Iino, M., Department of Pharmacology, Graduate School of Medicine, The University of Tokyo, Tokyo and  Department of Molecular Cell Physiology, The Tokyo Metropolitan Institute of Medical Science, calcineurin, Ca2+ oscillation, frequency dependence, NFAT, nuclear translocation "GFP–NFAT expressing BHK cells on poly-D-lysine-coated glass-bottomed dish (MatTek, Ashland, MA) were loaded at room temperature with 5 µM Fura-PE3AM (TefLabs, Austin, TX) in physiological salt solution (PSS) containing 150 mM NaCl, 4 mM KCl, 2 mM CaCl2, 
BICR-M1Rk Confocal 588 Mechanisms of Cx43 and Cx26 transport to the plasma membrane and gap junction regeneration Previous reports have suggested that Cx26 exhibits unique intracellular transport pathways en route to the cell surface compared with other members of the connexin family. To directly examine and compare nascent and steady-state delivery of Cx43 and Cx26  Inverted confocal microscope   Journal of Cell Science 118 19 October 2005